AC T02533
XX
ID T02533
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DT 10.12.1998 (created); ewi.
DT 08.03.2005 (updated); mkl.
CO Copyright (C), QIAGEN.
XX
FA AREA
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OS Aspergillus nidulans, Emericella nidulans
OC Eukaryota; Fungi; Ascomycota; Euascomycetes; Plectomycetes; Eurotiales; Trichocomaceae; Emericella.
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HO NIT2 (Neurospora crassa) [5].
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CL C0003; CC (g).
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SZ 876 AA; 94.2 kDa (gene) (calc.).
XX
SQ MSGLTLGGGSGRVRPTQAAAAFPTSHDFADPDRSSVARNANRPSRRFAAPPQLSDDFSLE
SQ SPTDSAQVHDNLLQDALFPEWKANSPRGVDSPDEMQKKDPLATQIWKLYSRTKAQLPNQE
SQ RMENLTWRMMALSLRRQERERAQQQARASSQKSPVPGMSGIAQLRLSDRVSNTPTTTADT
SQ VSDAMNLDDFIIPFSPSDHPSPSTTKASEATTGAIPIKARRDQSASEATPVPASFPHPAQ
SQ DQRRESEFGYVPRRVRKTSIDERQFFNLQIPSRKRPAESSPHVPPVSTSMLAHDPDFSHA
SQ VPEYTLDTSHGLSLQNQMNAQQLANAQNHTSPNMAFALDTFNLGDDPILPSAGPYQQQFT
SQ FSPSESPMTSGNPFANLYAQTPIASSLNSTDFFSPPPSGYQSTASTPQPAYDGEHSKYFD
SQ MPVDARSQRRVVPAYITQRSSNLSASLQPRYMYNQGGSSQDITQQNAHMGAQSSSMQSPG
SQ FSIPQHVDPTQVLNPNEFNGNHAAMFSFGADSDVEDDDGNQFSAGGLAMPAEFGDDSISD
SQ MNSNMAWETSYPNSFQSLPAFAAQHRKHVTIGSADMMDTPSEWNQGGSLGRTHESAASVS
SQ EVRNRDQDPRRQKIARTSSTPNTAQLLRQSMQNQSSHTSPNTPPESGLNSAAPSRPASPG
SQ GTKNGEQNGPTTCTNCFTQTTPLWRRNPEGQPLCNACGLFLKLHGVVRPLSLKTDVIKKR
SQ NRNSANSLAVGSSRVSKKSARKNSVQQVTPTAPTSSRAQSNTTSESPPAMPGSSGRGSGV
SQ VPIAAAPPKSSSAATTSPGTNNGCGAVQVAPKRQRRLEKASDVDMAESPSSTSSGGRSKV
SQ VPLAPAMPPAAVNPANHSIAAGQGASQEWEWLTMSL
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SC translated from EMBL #X52491
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FT 1 94 
PF07573; Nitrogen regulatory protein AreA N terminus.
FT 90 628 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 667 717 
SM00401; ZnF_GATA.
FT 667 720 
PS50114; GATA_ZN_FINGER_2.
FT 673 707 
PF00320; GATA zinc finger.
FT 865 876 
required for trans-activation [1].
XX
SF conflict: different sequence in [2];
SF replacement of the two zinc fingers of murine GATA-1 (202-305) by the single zinc finger of areA (658-724) creates a chimeric protein which is functional in induction of globin genes in cultured embryonal stem cells [3];
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FF activator [1];
FF mediating nitrogen metabolite repression in response to intracellular glutamine level [1];
FF under nitrogen derepression conditions, mRNA stability is five times enhanced due to some elements in the 3'-UTR [1];
FF Lack of AREA can be partially complemented by NIT2 from Neurospora cr. [5];
FF AREA also regulates Avr9 from Cladosporium fulvum [7];
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DR EMBL: X52491;
DR UniProtKB: P17429;
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RN [1]; RE0007094.
RX PUBMED: 8654376.
RA Platt A., Langdon T., Arst jr H. N., Kirk D., Tollervey D., Sanchez J. M. M., Caddick M. X.
RT Nitrogen metabolite signalling involves the C-terminus and the GATA domain of the Aspergillus transcription factor AREA and the 3' untranslated region of its mRNA
RL EMBO J. 15:2791-2801 (1996).
RN [2]; RE0007095.
RX PUBMED: 1970293.
RA Kudla B., Caddick M.X., Langdon T., Martinez-Rossi N.M., Bennett C. F., Sibley S., Davies R. W., Arst jr H. N.
RT The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger
RL EMBO J. 9:1355-1364 (1990).
RN [3]; RE0013446.
RX PUBMED: 7823931.
RA Blobel G. A., Simon M. C., Orkin S. H.
RT Rescue of GATA-1-deficient embryonic stem cells by heterologous GATA-binding proteins.
RL Mol. Cell. Biol. 15:626-633 (1995).
RN [4]; RE0013544.
RX PUBMED: 8596437.
RA Langdon T., Sheerins A., Ravagnani A., Gielkens M., Caddick M. X., Arst jr H. N.
RT Mutational analysis reveals dispensability of the N-terminal region of the Aspergillus transcription factor mediating nitrogen metabolite repression.
RL Mol. Microbiol. 17:877-888 (1995).
RN [5]; RE0013884.
RX PUBMED: 2954160.
RA Davis M. A., Hynes M. J.
RT Complementation of areA- regulatory gene mutations of Aspergillus nidulans by the heterologous regulatory gene nit-2 of Neurospora crassa.
RL Proc. Natl. Acad. Sci. USA 84:3753-3757 (1987).
RN [6]; RE0013910.
RX PUBMED: 10075929.
RA Muro-Pastor M. I., Gonzalez R., Strauss J., Narendja F., Scazzocchio C.
RT The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter
RL EMBO J. 18:1584-1597 (1999).
RN [7]; RE0015607.
RX PUBMED: 10394902.
RA Snoeijers S. S., Vossen P., Goosen T., Van den Broek H. W., De Wit P. J.
RT Transcription of the avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum is regulated by a GATA-type transcription factor in Aspergillus nidulans
RL Mol. Gen. Genet. 261:653-659 (1999).
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//