AC T00625
XX
ID T00625
XX
DT 09.11.1992 (created); ewi.
DT 10.10.2012 (updated); mkl.
CO Copyright (C), QIAGEN.
XX
FA ZEB (1124 AA)
XX
SY AREB6; Atp1a1 Regulatory Element Binding protein 6; deltaEF1; negative regulator of IL2; Nil-2-a; TCF8 (gene name); ZEB; zinc finger E-box-binding protein.
XX
OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
XX
GE G004664 ZEB1; HGNC: ZEB1.
XX
HO zfh-1 (Dros.) T00919; deltaEF1 (chick) T01467.
XX
CL C0021; CH+homeo.
XX
SZ 1124 AA; 124.1 kDa (cDNA) (calc.).
XX
SQ MADGPRCKRRKQANPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDD
SQ LPTDQTVLPGRSSEREGNAKNCWEDDRKEGQEILGPEAQADEAGCTVKDDECESDAENEQ
SQ NHDPNVEEFLQQQDTAVIFPEAPEEDQRQGTPEASGHDENGTPDAFSQLLTCPYCDRGYK
SQ RFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGRDQRHVTQSGCNRKF
SQ KCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLIPVNG
SQ RPRTGLKTSQCSSPSLSASPGSPTRPQIRQKIENKPLQEQLSVNQIKTEPVDYEFKPIVV
SQ ASGINCSTPLQNGVFTGGGPLQATSSPQGMVQAVVLPTVGLVSPISINLSDIQNVLKVAV
SQ DGNVIRQVLENNQANLASKEQETINASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLE
SQ QPSQLQVVPQNLKKENPVATNSCKSEKLPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGD
SQ INALPELKHYDLKQPTQPPPLPAAEAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYY
SQ ALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNND
SQ QPQSANANEPQDSTVNLQSPLKMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNTQGYL
SQ YTAEGAQEEPQVEPLDLSLPKQQGELLERSTITSVYQNSVYSVQEEPLNLSCAKKEPQKD
SQ SCVTDSEPVVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQ
SQ VAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTE
SQ NGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPY
SQ QCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEAGPEILSNEHVGARASPSQG
SQ DSDERESLTREEDEDSEKEEEEEDKEMEELQEEKECEKPQGDEEEEEEEEEVEEEEVEEA
SQ ENEGEEAKTEGLMKDDRAESQASSLGQKVGESSEQVSEEKTNEA
XX
SC Swiss-Prot#P37275
XX
FT 170 193 
PF00096; zf-C2H2.
FT 170 193 
SM00355; c2h2final6.
FT 170 198 
PS50157; ZINC_FINGER_C2H2_2.
FT 200 222 
PF00096; zf-C2H2.
FT 200 222 
SM00355; c2h2final6.
FT 200 227 
PS50157; ZINC_FINGER_C2H2_2.
FT 240 262 
PF00096; zf-C2H2.
FT 240 262 
SM00355; c2h2final6.
FT 240 267 
PS50157; ZINC_FINGER_C2H2_2.
FT 268 288 
SM00355; c2h2final6.
FT 268 292 PF00096; zf-C2H2.
FT 427 1053 PF00478; IMP dehydrogenase / GMP reductase domain.
FT 581 643 SM00389; HOX_1.
FT 754 901 hydrophobic repression domain [6].
FT 904 926 PF00096; zf-C2H2.
FT 904 926 SM00355; c2h2final6.
FT 904 931 PS50157; ZINC_FINGER_C2H2_2.
FT 932 954 PF00096; zf-C2H2.
FT 932 954 SM00355; c2h2final6.
FT 932 959 PS50157; ZINC_FINGER_C2H2_2.
FT 960 981 SM00355; c2h2final6.
FT 960 986 PF00096; zf-C2H2.
FT 960 988 PS50157; ZINC_FINGER_C2H2_2.
FT 1011 1124 acidic activation domain [6].
XX
SF ZEB (1124 AA) is the extended form of Nil-2-a [4];
SF Nil-2-a was originally reported to be a protein of 735 AA with an expected molecular weight of 80.5 kDa containing 3 zinc finger motifs;
SF zinc finger 4 is of C2HC-type, while the others are of C2H2-type [4];
SF S/T-rich region between the zinc finger domains [4];
SF the homeo domain is not directly involved in DNA binding but interacts with the N-terminal zinc finger cluster [5];
SF essential fingers for DNA binding are fingers 3 and 4 in the N-terminal zinc finger cluster and fingers 6 and 7 in the C-terminal zinc finger cluster [5];
SF multiple conformations with respect to DNA binding, C-terminal zinc finger cluster works as a positive transcriptional regulator whereas the N-terminal zinc finger cluster works as a negative transcriptional regulator [5];
SF interaction between the ZEB (1124 AA) repression domain and the general cofactor NC2 T00197 [6];
XX
CP T cells; heart [4]; skeletal muscle [4] [4].
XX
FF repressor of interleukin-2 expression [2];
FF repressor of the immunoglobulin heavy-chain enhancer [3];
FF represses promoter P1 of the p73 gene in proliferating cells [7];
FF transcriptional repression might be mediated by the negative cofactor NC2 T00197 [6];
XX
IN T00197 NC2; human, Homo sapiens.
XX
MX M00412 V$AREB6_01.
MX M00413 V$AREB6_02.
MX M00414 V$AREB6_03.
MX M00415 V$AREB6_04.
MX M00073 V$DELTAEF1_01.
MX M07371 V$ZEB_Q3.
XX
BS R09389.
BS R09390.
BS R09391.
BS R09392.
BS R09393.
BS R09394.
BS R09395.
BS R09396.
BS R09397.
BS R09398.
BS R09399.
BS R09400.
BS R09401.
BS R09402.
BS R09403.
BS R09404.
BS R09405.
BS R09406.
BS R09407.
BS R09408.
BS R09409.
BS R09410.
BS R09411.
BS R09412.
BS R09413.
BS R09414.
BS R09415.
BS R09416.
BS R09417.
BS R09418.
BS R09419.
BS R09420.
BS R09421.
BS R09422.
BS R09423.
BS R09424.
BS R09425.
BS R09426.
BS R09427.
BS R09428.
BS R09429.
BS R09430.
BS R09431.
BS R09432.
BS R09433.
BS R09434.
BS R09435.
BS R09436.
BS R09437.
BS R09438.
BS R09439.
BS R09440.
BS R09441.
BS R02304.
BS R25488.
BS R25489.
BS R25483.
BS R25487.
BS R15630.
BS R15631.
BS R15632.
BS R15633.
BS R15634.
BS R29065.
BS R04632.
XX
DR TRANSPATH: MO000025063.
DR EMBL: M81699;
DR EMBL: U19969;
DR UniProtKB: P37275;
XX
RN [1]; RE0000807.
RX PUBMED: 1427828.
RA Williams T. M., Montoya G., Wu Y., Eddy R. L., Byers M. G., Shows T. B.
RT The TCF8 gene encoding a zinc finger protein (Nil-2-a) resides on human chromosome 10p11.2
RL Genomics 14:194-196 (1992).
RN [2]; RE0002692.
RX PUBMED: 1840704.
RA Williams T. M., Moolten D., Burlein J., Romano J., Bhaerman R., Godillot A., Mellon M., Rauscher III F. J., Kant J. A.
RT Identification of a zinc finger protein that inhibits IL-2 gene expression
RL Science 254:1791-1794 (1991).
RN [3]; RE0003743.
RX PUBMED: 8065348.
RA Genetta T., Ruezinsky D., Kadesch T.
RT Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer
RL Mol. Cell. Biol. 14:6153-6163 (1994).
RN [4]; RE0006474.
RX PUBMED: 8138542.
RA Watanabe Y., Kawakami K., Hrayama Y., Nagano K.
RT Transcription factors positively and negatively regulating the Na,K-ATPase alpha1 subunit gene
RL J. Biochem. 114:849-855 (1993).
RN [5]; RE0011035.
RX PUBMED: 7588776.
RA Ikeda K., Kawakami K.
RT DNA binding through distinct domains of zinc-finger-homeodomain protein AREB6 has different effects on gene transcription
RL Eur. J. Biochem. 233:73-82 (1995).
RN [6]; RE0015154.
RX PUBMED: 9418848.
RA Ikeda K., Halle J. P., Stelzer G., Meisterernst M., Kawakami K.
RT Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6
RL Mol. Cell. Biol. 18:10-18 (1998).
RN [7]; RE0024757.
RX PUBMED: 11713281.
RA Fontemaggi G., Gurtner A., Strano S., Higashi Y., Sacchi A., Piaggio G., Blandino G.
RT The transcriptional repressor ZEB regulates p73 expression at the crossroad between proliferation and differentiation.
RL Mol. Cell. Biol. 21:8461-8470 (2001).
XX
//