AC T00699
XX
ID T00699
XX
DT 15.10.1992 (created); ewi.
DT 08.02.2006 (updated); vma.
CO Copyright (C), QIAGEN.
XX
FA Prd
XX
SY Paired; Prd.
XX
OS fruit fly, Drosophila melanogaster
OC eukaryota; animalia; metazoa; arthropoda; insecta; diptera; drosophiloidea; drosophilidae
XX
GE G021787 prd.
XX
CL C0018; paired-homeo.
XX
SZ 613 AA; 65.5 kDa (calc.), 65.5 kDa (DNA)
XX
SQ MTVTAFAAAMHRPFFNGYSTMQDMNSGQGRVNQLGGVFINGRPLPNNIRLKIVEMAADGI
SQ RPCVISRQLRVSHGCVSKILNRYQETGSIRPGVIGGSKPRIATPEIENRIEEYKRSSPGM
SQ FSWEIREKLIREGVCDRSTAPSVSAISRLVRGRDAPLDNDMSSASGSPAGDGTKASSSCG
SQ SDVSGGHHNNGKPSDEDISDCESEPGIALKRKQRRCRTTFSASQLDELERAFERTQYPDI
SQ YTREELAQRTNLTEARIQVWFSNRRARLRKQHTSVSGGAPGGAAASVSHVAASSSLPSVV
SQ SSVPSMAPLAMMPGSLDPATVYQQQYDFYGSHANISVSAAAPMASSNLSPGITTTPPHHH
SQ QFYNPSANTASYIMPGENGNTTPTGNIIVSSYETQLGSVYGTETETHQTMPRNESPNESV
SQ SSAFGQLPPTPNSLSAVVSGAGVTSSSGANSGADPSQSLANASAGSEELSAALKVESVDL
SQ IAASQSQLYGGWSSMQALRPNAPLSPEDSLNSTSSTSQALDVTAHQMFHPYQHTPQYASY
SQ PAPGHAHSHHGHPHAPHPHAHPHPQYAGAHPHYPPPSSSAHFMPQNFNAAAFPSPSKVNY
SQ TTMPPQPFYPSWY
XX
SC Swiss-Prot#P06601
XX
FT 27 151 
PF00292; 'Paired box' domain.
FT 27 151 
SM00351; pax3.
FT 27 153 
PS51057; PAIRED_2.
FT 211 271 
PS50071; HOMEOBOX_2.
FT 213 269 
PS50552; PAX.
FT 213 275 
SM00389; HOX_1.
FT 214 270 
PF00046; Homeobox domain.
XX
SF pair rule gene class [8];
SF Prd(Q->K in position 9 of helix 3): gain of Ftz-like binding specificity [1];
SF distinct binding specificity for paired box and homeodomain [1] [4];
SF paired domain has two subdomains of three alpha-helices each [5];
SF DNA-contacts by helix 3, helices 1 and 2 antiparallel against each other and nearly perpendicular to helix 3 [5];
SF additional minor groove contacts by type II beta turn (39-42) and by the C-terminal tail of the N-terminal subdomain (91-98) [5];
SF no DNA-contacts observed by the similarly structured C-terminal subdomain [5];
SF typical fold of the homeo domain, direct contacts of the recognition helix plus extensive network of water molecules mediating additional major groove contacts of the recognition helix [6];
SF distortions of DNA conformation by the first bound molecule cause cooperative binding of a second one, both contacting each other through their homeo domains [6];
SF Gly-41->Ser mutation (analogous to undulated mutant in mice) abolishes DNA-binding by paired domain [4];
XX
CP (embryo 2-4h old:) blastoderm and early gastrula [8].
XX
FF regulation of anterior-posterior segmentation [8];
FF activator [3];
FF pair-rule gene product [3];
XX
IN T00295 Ftz; fruit fly, Drosophila melanogaster.
XX
MX M07874 I$PRD_02.
MX M01091 I$PRD_Q6.
XX
BS R01669.
BS R02485.
BS R02810.
BS R02811.
BS R00411.
BS R02801.
BS R02809.
BS R17414.
BS R17415.
BS R17418.
BS R17419.
BS R17420.
BS R17423.
BS R17426.
BS R17429.
BS R17430.
BS R02484.
XX
DR TRANSPATH: MO000045954.
DR EMBL: M14548; DMPRD.
DR UniProtKB: P06601; HMPR_DROME.
DR FLYBASE: FBgn0003145.
XX
RN [1]; RE0000066.
RX PUBMED: 2572327.
RA Treisman J., Goenczy P., Vashishta M., Harris E., Desplan C.
RT A single amino acid can determine the DNA binding specificity of homeodomain proteins
RL Cell 59:553-562 (1989).
RN [2]; RE0001777.
RX PUBMED: 2895896.
RA Hoey T., Levine M.
RT Divergent homeo box proteins recognize similar DNA sequences in Drosophila
RL Nature 332:858-861 (1988).
RN [3]; RE0002802.
RX PUBMED: 1679407.
RA Morrissey D., Askew D., Raj L., Weir M.
RT Functional dissection of the paired segmentation gene in Drosophila embryos
RL Genes Dev. 5:1684-1696 (1991).
RN [4]; RE0002805.
RX PUBMED: 1672661.
RA Treisman J., Harris E., Desplan C.
RT The paired box encodes a second DNA-binding domain in the Paired homeo domain protein
RL Genes Dev. 5:594-604 (1991).
RN [5]; RE0004177.
RX PUBMED: 7867071.
RA Xu W., Rould M. A., Jun S., Desplan C., Pabo C. O.
RT Crystal structure of a paired domain-DNA complex at 2.5 A resolution reveals structural basis for Pax developmental mutations
RL Cell 80:639-650 (1995).
RN [6]; RE0004179.
RX PUBMED: 7671301.
RA Wilson D. S., Guenther B., Desplan C., Kuriyan J.
RT High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA
RL Cell 82:709-719 (1995).
RN [7]; RE0004181.
RX PUBMED: 2877746.
RA Frigerio G., Burri M., Bopp D., Baumgartner S., Noll M.
RT Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network
RL Cell 47:735-746 (1986).
RN [8]; RE0014029.
RA Kilchherr F., Baumgartner S., Bopp D., Frei E., Noll M.
RT Isolation of the paired gene of Drosophila and its spatial expression during early embryogenesis
RL Nature 321:493-499 (1986).
XX
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