AC T02162
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ID T02162
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DT 26.05.1997 (created); hiwi.
DT 05.05.2008 (updated); grs.
CO Copyright (C), QIAGEN.
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FA TFIIE-alpha
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SY general transcription factor IIE 56 kDa subunit; GTF2E1; p56; TF2E1; TFIIE-alpha; transcription factor IIE 56 kDa subunit; transcription initiation factor IIE, alpha subunit.
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OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
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GE G006185 GTF2E1; HGNC: GTF2E1.
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SF acidic subunit of TFIIE, two chains of which bind to a homodimer of the beta subunit [4];
SF experimentally confirmed zinc-binding motif [6];
SF zinc-binding motif resembles the one found in the UvrA and UvrB components of the bacterial excision repair endonuclease complex [1] [4];
SF the zinc finger motif is essential to support basal transcription and to assemble into the transcription complex, though not for interaction with the beta-subunit [6];
SF TFIIE-alpha interacts with the C-terminal domain (CTD) of the largest RNA pol II subunit [7];
SF the N-terminal region of TFIIE-alpha including a small leucine repeat, the zinc finger structure, and the predicted helix-turn-helix motif, is required and sufficient for most biological functions (transcription activation, TFIIE-beta interaction, phosphorylation of the CTD);
SF additional strong stimulatory influence and interaction with TFIIH, however, is exerted by an acidic region within the C-terminal part of the molecule [8];
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FF transcription activation [2] [3];
FF required for phosphorylation of the CTD of pol II [5];
FF phosphorylated by TFIIH [5];
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IN T00783 TAFII70-alpha; human, Homo sapiens.
IN T02162 TFIIE-alpha; human, Homo sapiens.
IN T02163 TFIIE-beta; human, Homo sapiens.
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DR TRANSPATH: MO000013292.
DR UniProtKB: P29083;
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RN [1]; RE0005714.
RX PUBMED: 1956403.
RA Ohkuma Y., Sumimoto H., Hoffmann A., Shimasaki S., Horikoshi M., Roeder R. G.
RT Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE
RL Nature 398-401:354 (1991).
RN [2]; RE0005716.
RX PUBMED: 2251258.
RA Ohkuma Y., Sumimoto H., Horikoshi M., Roeder R. G.
RT Factors involved in specific transcription by mammalian RNA polymerase II: purification and characterization of general transcription factor TFIIE
RL Proc. Natl. Acad. Sci. USA 87:9163-9167 (1990).
RN [3]; RE0005717.
RX PUBMED: 2026628.
RA Inostroza J., Flores O., Reinberg D.
RT Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE
RL J. Biol. Chem. 266:9304-9308 (1991).
RN [4]; RE0005718.
RX PUBMED: 1956398.
RA Peterson M. G., Inostroza J., Maxon M. E., Flores O., Admon A., Reinberg D., Tjian R.
RT Structure and functional properties of human general transcription factor IIE
RL Nature 354:369-373 (1991).
RN [5]; RE0005726.
RX PUBMED: 8166891.
RA Ohkuma Y., Roeder R. G.
RT Regulation of TFIIH ATPase and kinase activities by TFIIE during active initiation complex formation
RL Nature 368:160-163 (1994).
RN [6]; RE0005728.
RX PUBMED: 7937800.
RA Maxon M. E., Tjian R.
RT Transcriptional activity of transcription factor IIE is dependent on zinc binding
RL Proc. Natl. Acad. Sci. USA 91:9529-9533 (1994).
RN [7]; RE0005730.
RX PUBMED: 7559497.
RA Kang M. E., Dahmus M. E.
RT The photochemical cross-linking of recombinant C-terminal domain to proteins in a HeLa cell transcription extract that comigrate with transcription factor IIE and IIF
RL J. Biol. Chem. 270:23390-23397 (1995).
RN [8]; RE0005731.
RX PUBMED: 7651404.
RA Ohkuma Y., Hashimoto S., Wang K., Horikoshi M., Roeder R. G.
RT Analysis of the role of TFIIE in basal transcription and TFIIH-mediated carboxy-terminal domain phosphorylation through structure-function studies of TFIIE-alpha
RL Mol. Cell. Biol. 15:4856-4866 (1995).
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