AC T02227
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ID T02227
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DT 24.10.1997 (created); ewi.
DT 29.11.2012 (updated); mkl.
CO Copyright (C), QIAGEN.
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FA Toa1p
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SY TFIIA (32 kDa subunit); TOA1; TOA1p; YOR194C.
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OS yeast, Saccharomyces cerevisiae
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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GE G004249 TOA1.
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HO TFIIA-alpha/beta (human), TFII-L (Drosophila).
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SZ 286 AA; 32.2 kDa (gene) (calc.), 32 kDa (SDS) [2], 43 kDa (SDS) [1] [1] [2]
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SQ MSNAEASRVYEIIVESVVNEVREDFENAGIDEQTLQDLKNIWQKKLTETKVTTFSWDNQF
SQ NEGNINGVQNDLNFNLATPGVNSSEFNIKEENTGNEGLILPNINSNNNIPHSGETNINTN
SQ TVEATNNSGATLNTNTSGNTNADVTSQPKIEVKPEIELTINNANITTVENIDDESEKKDD
SQ EEKEEDVEKTRKEKEQIEQVKLQAKKEKRSALLDTDEVGSELDDSDDDYLISEGEEDGPD
SQ ENLMLCLYDKVTRTKARWKCSLKDGVVTINRNDYTFQKAQVEAEWV
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SC Swiss-Prot#P32773
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FT 1 54 
required for subunit association and TBP-TATA-binding [3].
FT 1 285 
PF03153; Transcription factor IIA, alpha/beta subunit.
FT 217 286 
required for TBP-TATA-binding [3].
FT 241 286 
required for subunit association [3].
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SF pI(calc.) = 4.1 [1];
SF outmost N- and C-terminal parts exhibit significant homology with corresponding parts of human TFIIA-alpha/beta (39% and 44% identity, resp.) and are important for TFIIA function [3];
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IN T02228 Toa2p; yeast, Saccharomyces cerevisiae.
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BS R01015.
BS R01016.
BS R00970.
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DR EMBL: M85248; SCTFIIAA.
DR UniProtKB: P32773; TOA1_YEAST.
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RN [1]; RE0005668.
RX PUBMED: 1546313.
RA Ranish J. A., Lane W. S., Hahn S.
RT Isolation of two genes that encode subunits of the yeast transcription factor IIA
RL Science 255:1127-1129 (1992).
RN [2]; RE0005669.
RX PUBMED: 1918049.
RA Ranish J. A., Hahn S.
RT The yeast general transcription factor TFIIA is composed of two polypeptide subunits
RL J. Biol. Chem. 266:19320-19327 (1991).
RN [3]; RE0005672.
RX PUBMED: 7862117.
RA Kang J. J., Auble D. T., Ranish J. A., Hahn S.
RT Analysis of the yeast transcription factor TFIIA: Distinct functional regions and a polymerase II-specific role in basal and activated transcription
RL Mol. Cell. Biol. 15:1234-1243 (1995).
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