AC T05132
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ID T05132
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DT 27.05.2002 (created); mas.
DT 03.12.2010 (updated); mkl.
CO Copyright (C), QIAGEN.
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FA GLIS2
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SY GLI-Kruppel family member GLI5; Gli5; Glis 2; Glis-2; Glis2; Klf16; Krueppel-like Zinc Finger Protein Glis2; MGC36775; Nkl.
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OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
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GE G004146 Glis2.
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CL C0001; CH.
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SZ 521 AA; 55.9 kDa (calc.).
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SQ MHSLDEPLDLKLSITKLRAAREKRERTLGVVRHHALHRELGLVDDSPAPGSPGSPPPGFL
SQ LNPKFPEKVDGRFSAAPLVDLSLSPPSGLDSPNGSSSLSPECQGNGDLPPLPTAVDFQPL
SQ RYLDGVPSSFQFFLPLGSGGALHLPASSFLPPPKDKCLSPELPLAKQLVCRWAKCNQLFE
SQ LLQDLVDHVNDHHVKPEQDARYCCHWEDCARHGRGFNARYKMLIHIRTHTNEKPHRCPTC
SQ NKSFSRLENLKIHNRSHTGEKPYVCPYEGCNKRYSNSSDRFKHTRTHYVDKPYYCKMPGC
SQ HKRYTDPSSLRKHIKAHGHFVSHEQQELLQLRPPPKPPLPTPDSGSYVSGAQIIIPNPAA
SQ LFGGPSLPGLPLPLPPGPLDLSALACGNGGGGGGGIGPGLPGSVLPLNLAKNPLLPSPFG
SQ AGGLGLPVVSLLGGSAGSKAEGEKGRGSVPARVLGLENHKTPLERTERSRSRPSPDGLPL
SQ LPGTVLDLSTGNSAASSPEVLTPGWVVIPPGSVLLKPAVVN
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SC translated from EMBL #AF325913
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FT 21 26 
basic region (4/6) [2].
FT 30 148 
contains activation domain, functional [2].
FT 148 171 
contains repression domain, functional [2].
FT 168 193 
SM00355; c2h2final6.
FT 170 193 
zinc finger1, C2H2-type (C-X2-4-C-X12-15-H-X3-4-H) [4].
FT 171 193 
contains repression domain, functional [2].
FT 202 229 
SM00355; c2h2final6.
FT 204 229 
zinc finger2, C2H2-type (C-X2-4-C-X12-15-H-X3-4-H) [4].
FT 207 234 
PS50157; ZINC_FINGER_C2H2_2.
FT 235 257 
PF00096; zf-C2H2.
FT 235 257 SM00355; c2h2final6.
FT 235 262 PS50157; ZINC_FINGER_C2H2_2.
FT 237 257 zinc finger3, C2H2-type (C-X2-4-C-X12-15-H-X3-4-H) [4].
FT 263 287 PF00096; zf-C2H2.
FT 263 287 SM00355; c2h2final6.
FT 263 292 PS50157; ZINC_FINGER_C2H2_2.
FT 265 286 zinc finger4, C2H2-type (C-X2-4-C-X12-15-H-X3-4-H) [4].
FT 293 317 PF00096; zf-C2H2.
FT 293 317 PS50157; ZINC_FINGER_C2H2_2.
FT 293 317 SM00355; c2h2final6.
FT 295 318 zinc finger5, C2H2-type (C-X2-4-C-X12-15-H-X3-4-H) [4].
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SF contains 5 zinc finger domains [2];
SF closely related to GLI and ZIC subfamily of Krueppel-like zinc finger transcription factors [2];
SF high amino acid identity to GLI and ZIC family members in zinc finger domain (pos. 170-317) (49-57%) with highest identities in zinc finger 3 and 5 [2];
SF contains functional repression domain within zinc finger 1 [2];
SF 93% amino acid identity between human T05131 and mouse ortholog T05132 [1];
SF the zinc finger domains are highly homologous with chick NKL T06400 94% and frog NKL T06401 67% [3];
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CP (adult): high levels in kidney and lower levels in heart, lung [2]; weak expression in prostate, colon, brain [2]; E9,5: cranial ganglia, dorsal root ganglia, neural tube, E10,5: broadly intermediate zone of the hindbrain, spinal cord, dorsal root ganglia, E12,5: ventricular zone [3] [2] [3].
EX brain,,,adult; low; Northern blot; total RNA; [2].
EX brain,,,adult; low; RT-PCR; RNA (undefined); [2].
EX colon,,,adult; low; Northern blot; total RNA; [2].
EX colon,,,adult; low; RT-PCR; RNA (undefined); [2].
EX heart,,,adult; low; Northern blot; total RNA; [2].
EX heart,,,adult; medium; RT-PCR; RNA (undefined); [2].
EX intestine,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX intestine,,,adult; low; Northern blot; total RNA; [2].
EX kidney (right and left),,,adult; high; RT-PCR; RNA (undefined); [2].
EX kidney (right and left),,,adult; very high; Northern blot; total RNA; [2].
EX liver,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX liver,,,adult; low; Northern blot; total RNA; [2].
EX lung,,,adult; medium; Northern blot; total RNA; [2].
EX lung,,,adult; medium; RT-PCR; RNA (undefined); [2].
EX mammary gland,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX mammary gland,,,adult; low; Northern blot; total RNA; [2].
EX muscles,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX muscles,,,adult; low; Northern blot; total RNA; [2].
EX oesophagus,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX oesophagus,,,adult; low; Northern blot; total RNA; [2].
EX pancreas,,,adult; low; Northern blot; total RNA; [2].
EX prostate gland,,,adult; low; Northern blot; total RNA; [2].
EX prostate gland,,,adult; low; RT-PCR; RNA (undefined); [2].
EX spleen,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX spleen,,,adult; low; Northern blot; total RNA; [2].
EX testis (right and left),,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX testis (right and left),,,adult; low; Northern blot; total RNA; [2].
EX thymus,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX thymus,,,adult; low; Northern blot; total RNA; [2].
EX thyroid gland,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX thyroid gland,,,adult; low; Northern blot; total RNA; [2].
EX trachea,,,adult; detectable; RT-PCR; RNA (undefined); [2].
EX trachea,,,adult; low; Northern blot; total RNA; [2].
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FF can function as repressor of basal transcription but contains both functional repression domains and functional autonomous transactivation domain [2];
FF transactivation and repressional activity cell-type dependent (e.g. activation domain increases transcriptional activity 200-fold in 293 cells but only 7-fold in MDCK cells) which may be due different expression levels of co-activators and co-repressors [2];
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MX M02759 V$GLIS2_03.
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BS R16214.
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DR TRANSPATH: MO000032976.
DR UniProtKB: Q8VDL9;
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RN [1]; RE0017825.
RX PUBMED: 11738817.
RA Zhang F., Jetten A. M.
RT Genomic structure of the gene encoding the human GLI-related, Kruppel-like zinc finger protein GLIS2
RL Gene 280:49-57 (2001).
RN [2]; RE0017826.
RX PUBMED: 11741991.
RA Zhang F., Nakanishi G., Kurebayashi S., Yoshino K., Perantoni A., Kim Y. S., Jetten A. M.
RT Characterization of Glis2, a novel gene encoding a Gli-related, Kruppel-like transcription factor with transactivation and repressor functions. Roles in kidney development and neurogenesis
RL J. Biol. Chem. 277:10139-10149 (2002).
RN [3]; RE0025320.
RX PUBMED: 11262234.
RA Lamar E., Kintner C., Goulding M.
RT Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation.
RL Development 128:1335-1346 (2001).
RN [4]; RE0025419.
RX PUBMED: 12042312.
RA Kim Y. S., Lewandoski M., Perantoni A. O., Kurebayashi S., Nakanishi G., Jetten A. M.
RT Identification of Glis1, a novel Gli-related, Kruppel-like zinc finger protein containing transactivation and repressor functions.
RL J. Biol. Chem. 277:30901-30913 (2002).
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