AC T14127
XX
ID T14127
XX
DT 01.01.1999 (created); ili.
DT 11.08.2014 (updated); yre.
CO Copyright (C), QIAGEN.
XX
FA SATB1-isoform1
XX
SY special AT-rich sequence binding protein 1.
XX
OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
XX
GE G002308 SATB1; HGNC: SATB1.
XX
HO murine SATB1-isoform1.
XX
CL C0006; homeo; 3.1.9.3.1.1.
XX
SZ 763 AA; 86.0 kDa (cDNA) (calc.), ca. 90 kDa (SDS) [2], 96-97 kDa (SDS) [5], 103 kDa (SDS) [1] [5] [1] [2]
XX
SQ MDHLNEATQGKEHSEMSNNVSDPKGPPAKIARLEQNGSPLGRGRLGSTGAKMQGVPLKHS
SQ GHLMKTNLRKGTMLPVFCVVEHYENAIEYDCKEEHAEFVLVRKDMLFNQLIEMALLSLGY
SQ SHSSAAQAKGLIQVGKWNPVPLSYVTDAPDATVADMLQDVYHVVTLKIQLHSCPKLEDLP
SQ PEQWSHTTVRNALKDLLKDMNQSSLAKECPLSQSMISSIVNSTYYANVSAAKCQEFGRWY
SQ KHFKKTKDMMVEMDSLSELSQQGANHVNFGQQPVPGNTAEQPPSPAQLSHGSQPSVRTPL
SQ PNLHPGLVSTPISPQLVNQQLVMAQLLNQQYAVNRLLAQQSLNQQYLNHPPPVSRSMNKP
SQ LEQQVSTNTEVSSEIYQWVRDELKRAGISQAVFARVAFNRTQGLLSEILRKEEDPKTASQ
SQ SLLVNLRAMQNFLQLPEAERDRIYQDERERSLNAASAMGPAPLISTPPSRPPQVKTATIA
SQ TERNGKPENNTMNINASIYDEIQQEMKRAKVSQALFAKVAATKSQGWLCELLRWKEDPSP
SQ ENRTLWENLSMIRRFLSLPQPERDAIYEQESNAVHHHGDRPPHIIHVPAEQIQQQQQQQQ
SQ QQQQQQQAPPPPQPQQQPQTGPRLPPRQPTVASPAESDEENRQKTRPRTKISVEALGILQ
SQ SFIQDVGLYPDEEAIQTLSAQLDLPKYTIIKFFQNQRYYLKHHGKLKDNSGLEVDVAEYK
SQ EEELLKDLEESVQDKNTNTLFSVKLEEELSVEGNTDINTDLKD
XX
SC Swiss-Prot#Q01826-1
XX
FT 182 701 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 346 495 
DNA-binding domain [1].
FT 361 448 
PS51042; CUT.
FT 362 448 
PF02376; CUT domain.
FT 370 445 
similarity to Cut- and Clox-homeo-proteins of Drosophila and mammals [3].
FT 484 571 
PF02376; CUT domain.
FT 484 571 
PS51042; CUT.
FT 493 568 
similarity to Cut- and Clox-homeo-proteins of Drosophila and mammals [3].
FT 593 607 
Q-stretch [2].
FT 641 702 
homeodomain-like domain [3].
FT 642 703 PS50071; HOMEOBOX_2.
FT 642 704 PS50560; CUT_HOMEODOMAIN.
FT 644 707 SM00389; HOX_1.
FT 645 702 PF00046; Homeobox domain.
XX
SF phosphorylated [1];
SF binds as a monomer [1];
SF appears to be directed by its homeodomain-like domain toward a preferential recognition of the core unwinding element [3];
XX
CP predominantly in thymus [2]; minute amounts in brain [2]; very low in testis [2]; HL60 [1] [6]; Jurkat [4]; K562 [5] [6]; U937, CEM, Namalwa, KG1, fetal liver, adult bone marrow [6] [5] [6] [1] [2] [4].
CN breast cancer cell line SK-BR-3 [4]; HeLa [6] [6] [4].
XX
FF binds to minor groove with little contact to DNA bases [2];
FF does not bind to ssDNA [2];
FF contacts AT-rich sequences flanked by sequences in which 1 strand contains only C, A & T [2];
FF these AT-rich sequences must have unpairing properties [1];
FF a small quantity remains extractable after stabilization procedures [5];
FF caution: distribution changes according to the stabilizing treatment used during matrix preparation [5];
FF not affected by apoptosis in hematopoietic cell line, but proteolyzed in apoptotic thymocytes [7];
XX
IN T04734 LUN-isoform1; human, Homo sapiens.
IN T22884 SATB2; Mammalia.
IN T16034 Sox-2; mouse, Mus musculus.
XX
MX M01723 V$SATB1_Q3.
XX
BS R32418.
BS R23361.
BS R23365.
BS R23367.
BS R03635.
XX
DR TRANSPATH: MO000056081.
DR SMARTDB: SB000012.
DR EMBL: M97287; HSSATB1A.
DR UniProtKB: Q01826-1;
XX
RN [1]; RE0006986.
RX PUBMED: 8114718.
RA Nakagomi K., Kohwi Y., Dickinson L. A., Kohwi-Shigematsu T.
RT A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1
RL Mol. Biol. Cell 14:1852-1860 (1994).
RN [2]; RE0006988.
RX PUBMED: 1505028.
RA Dickinson L. A., Joh T., Kohwi Y., Kohwi-Shigematsu T.
RT A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition
RL Cell 70:631-645 (1992).
RN [3]; RE0007006.
RX PUBMED: 9111059.
RA Dickinson L. A., Dickinson C. D., Kohwi-Shigematsu T.
RT An atypical homeodomain in SATB1 promotes specific recognition of the key structural element in a matrix attachment region
RL J. Biol. Chem. 272:11463-11470 (1997).
RN [4]; RE0007048.
RX PUBMED: 9548713.
RA deBelle I., Cai S., Kohwi-Shigematsu T.
RT The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops
RL J. Cell Biol. 141:335-348 (1998).
RN [5]; RE0013347.
RX PUBMED: 9215557.
RA Neri L. M., Fackelmayer F. O., Zweyer M., Kohwi-Shigematsu T., Martelli A. M.
RT Subnuclear localization of S/MAR-binding proteins is differently affected by in vitro stabilization with heat or Cu2+
RL Chromosoma 106:81-93 (1997).
RN [6]; RE0013698.
RX PUBMED: 8049444.
RA Cunningham J. M., Purucker M. E., Jane S. M., Safer B., Vanin E. F., Ney P. A., Lowrey C. H., Nienhuis A. W.
RT The regulatory element 3' to the gammaA-globin gene binds to the nuclear matrix and interacts with special A-T-rich binding protein (SATB1), an SAR/MAR-associating region DNA binding protein
RL Blood 84:1298-1308 (1994).
RN [7]; RE0017467.
RX PUBMED: 10025665.
RA Martelli A. M., Bortul R., Fackelmayer F. O., Tazzari P. L., Bareggi R., Narducci P., Zweyer M.
RT Biochemical and morphological characterization of the nuclear matrix from apoptotic HL-60 cells
RL J. Cell. Biochem. 72:35-46 (1999).
RN [8]; RE0023899.
RX PUBMED: 11937547.
RA Kieffer L. J., Greally J. M., Landres I., Nag S., Nakajima Y., Kohwi-Shigematsu T., Kavathas P. B.
RT Identification of a candidate regulatory region in the human CD8 gene complex by colocalization of DNase I hypersensitive sites and matrix attachment regions which bind SATB1 and GATA-3
RL J. Immunol. 168:3915-3922 (2002).
RN [9]; RE0067926.
RX PUBMED: 18408014.
RA Tan J. A., Sun Y., Song J., Chen Y., Krontiris T. G., Durrin L. K.
RT SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage.
RL J. Biol. Chem. 283:18124-18134 (2008).
RN [10]; RE0070893.
RX PUBMED: 20351170.
RA Tan J. A., Song J., Chen Y., Durrin L. K.
RT Phosphorylation-dependent interaction of SATB1 and PIAS1 directs SUMO-regulated caspase cleavage of SATB1.
RL Mol. Cell. Biol. 30:2823-2836 (2010).
XX
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