TRANSFAC FACTOR TABLE, Release 2017.2 - public - 2017-06-30, (C) QIAGEN

AC T00253 XX ID T00253 XX DT 15.10.1992 (created); ewi. CO Copyright (C), QIAGEN. XX FA En XX SY En; Engrailed. XX OS fruit fly, Drosophila melanogaster OC eukaryota; animalia; metazoa; arthropoda; insecta; diptera; drosophiloidea; drosophilidae XX GE G000098 en. XX HO En-1, En-2 (vertebrates). XX CL C0006; homeo. XX SZ 552 AA; 59.4 kDa (cDNA) (calc.), 69 kDa (SDS) XX SQ MALEDRCSPQSAPSPITLQMQHLHHQQQQQQQQQQQMQHLHQLQQLQQLHQQQLAAGVFH SQ HPAMAFDAAAAAAAAAAAAAAHAHAAALQQRLSGSGSPASCSTPASSTPLTIKEEESDSV SQ IGDMSFHNQTHTTNEEEEAEEDDDIDVDVDDTSAGGRLPPPAHQQQSTAKPSLAFSISNI SQ LSDRFGDVQKPGKSIENQASIFRPFEANRSQTATPSAFTRVDLLEFSRQQQAAAAAATAA SQ MMLERANFLNCFNPAAYPRIHEEIVQSRLRRSAANAVIPPPMSSKMSDANPEKSALGSLC SQ KAVSQIGQPAAPTMTQPPLSSSASSLASPPPASNASTISSTSSVATSSSSSSSGCSSAAS SQ SLNSSPSSRLGASGSGVNASSPQPQPIPPPSAVSRDSGMESSDDTRSETGSTTTEGGKNE SQ MWPAWVYCTRYSDRPSSGPRYRRPKQPKDKTNDEKRPRTAFSSEQLARLKREFNENRYLT SQ ERRRQQLSSELGLNEAQIKIWFQNKRAKIKKSTGSKNPLALQLMAQGLYNHTTVPLTKEE SQ EELEMRMNGQIP XX SC Swiss-Prot#P02836 XX FT 452 512 PS50071; HOMEOBOX_2. FT 454 516 SM00389; HOX_1. FT 455 511 PF00046; Homeobox domain. XX SF specific DNA-binding through the homeo domain [1]; SF overlapping DNA-binding specificity with Ftz [11] [1] [5]; SF cooperative DNA-binding with Exd [10]; SF crystal structure (co-crystals with DNA): three alpha-helices, helix 3 operating as DNA recognition helix binding to the major groove [7]; SF conserved residues W501 and F502 are part of a hydrophobic core between the backs of the nearly perpendicularly arranged helices 3 and 1 [7]; SF 8 AA N-terminal to helix 1 bind to the minor groove [7]; SF transferable Ala-rich repressor domain of 55 AA [13] [8]; SF En may also possess activating domains [13]; XX FF active repressor; FF segment polarity gene [6] [8]; FF also competing with TFIID for TATA-binding, thereby repressing transcription [9]; FF for gene repression, it depends on the simultaneous presence of transcription activators [13]; FF expression is initially activated by pair-rule gene products (e. g., Ftz, Prd, Zen) which may act synergistically [16] [18] [19]; FF probably through an indirect mechanism, En activates its own transcription [3]; FF subsequently, the gene wingless (wg) encodes a signalling molecule in neighboring cells which is required for maintenance of en expression giving rise to a positive autoregulation of En [17]; FF later, autoregulation becomes wg-independent [17]; XX IN T01482 Exd; fruit fly, Drosophila melanogaster. IN T02451 groucho; fruit fly, Drosophila melanogaster. XX MX M02338 I$EN_01. MX M07791 I$EN_02. MX M00696 I$EN_Q6. XX BS R00998. BS R01670. BS R02483. BS R09578. BS R17977. BS R17978. BS R17979. BS R17980. BS R17981. BS R00411. BS R00412. BS R00414. BS R01781. BS R01782. BS R01783. BS R00757. BS R17928. BS R17929. BS R17930. BS R17931. BS R17932. BS R17933. BS R17934. BS R17922. BS R17923. BS R17924. BS R17925. BS R17926. BS R17927. BS R01528. BS R01529. BS R17349. BS R17357. BS R17581. BS R17582. BS R17585. BS R01784. BS R01785. BS R01786. BS R02507. XX DR TRANSPATH: MO000024806. DR EMBL: K03055; DR EMBL: K03056; DR EMBL: K03057; DR EMBL: K03058; DR EMBL: M10017; DR EMBL: M29285; DR UniProtKB: P02836; DR FLYBASE: FBgn0000577; en. DR PDB: 1enh. DR PDB: 1hdd. XX RN [1]; RE0000063. RX PUBMED: 3046753. RA Desplan C., Theis J., O'Farrell P. H. RT The sequence specificity of homeodomain-DNA interaction RL Cell 54:1081-1090 (1988). RN [2]; RE0000131. RX PUBMED: 2897243. RA Biggin M. D., Tjian R. RT Transcription factors that activate the Ultrabithorax promoter in developmentally staged extracts RL Cell 53:699-711 (1988). RN [3]; RE0001403. RX PUBMED: 2573829. RA Kassis J. A., Desplan C., Wright D. K., O'Farrell P. H. RT Evolutionary Conservation of Homeodomain-Binding Sites and Other Sequences Upstream and within the Major Transcription Unit of the Drosophila Segmentation Gene engrailed RL Mol. Cell. Biol. 9:4304-4311 (1989). RN [4]; RE0001936. RX PUBMED: 1350328. RA Patel N. H., Ball E. E., Goodman C. S. RT Changing role of even-skipped during the evolution of insect pattern formation RL Nature 357:339-342 (1992). RN [5]; RE0002306. RX PUBMED: 2879282. RA Fainsod A., Bogarad L. D., Ruusala T., Lubin M., Crothers D. M., Ruddle F. H. RT The homeo domain of a murine protein binds 5' to its own homeo box RL Proc. Natl. Acad. Sci. USA 83:9532-9536 (1986). RN [6]; RE0002736. RX PUBMED: 1970761. RA Ohkuma Y., Horikoshi M., Roeder R. G., Desplan C. RT Binding site-dependent direct activation and repression of in vitro transcription by Drosophila homeodomain proteins RL Cell 61:475-484 (1990). RN [7]; RE0002779. RX PUBMED: 1977522. RA Kissinger C. R., Liu B., Martin-Blanco E., Kornberg T. B., Pabo C. O. RT Crystal structure of an engrailed homeodomain-DNA complex at 2.8 A resolution: a framework for understanding homeodomain-DNA interactions RL Cell 63:579-590 (1990). RN [8]; RE0002794. RX PUBMED: 1673924. RA Jaynes J. B., O'Farrell P. H. RT Active repression of transcription by the Engrailed homeodomain protein RL EMBO J. 10:1427-1433 (1991). RN [9]; RE0002826. RX PUBMED: 1969159. RA Ohkuma Y., Horikoshi M., Roeder R. G., Desplan C. RT Engrailed, a homeodomain protein, can repress in vitro transcription by competition with the TATA box-binding protein transcription factor IID RL Proc. Natl. Acad. Sci. USA 87:2289-2293 (1990). RN [10]; RE0004954. RX PUBMED: 7915200. RA van Dijk M. A., Murre C. RT extradenticle raises the DNA binding specificity of homeotic selector gene products RL Cell 78:617-624 (1994). RN [11]; RE0005033. RX PUBMED: 4079979. RA Desplan C., Theis J., Farrell P. H. RT The Drosophila development gene engrailed encodes a sequence specific DNA-binding activity RL Nature 318:630-835 (1985). RN [12]; RE0005034. RX PUBMED: 3917855. RA Poole S. J., Kauvar L. M., Drees B., Kornberg T. RT The engrailed locus of Drosophila: Structural analysis of an embryonic transcript. RL Cell 40:37-43 (1985). RN [13]; RE0005035. RX PUBMED: 8334991. RA Han K., Manley J. L. RT Functional domains of the Drosophila Engrailed protein RL EMBO J. 12:2723-2733 (1993). RN [14]; RE0005036. RX PUBMED: 3935318. RA DiNardo S., Kuner J., Theis J., Farrell P. H. RT Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein RL Cell 43:59-69 (1985). RN [15]; RE0005037. RX PUBMED: 4069216. RA Weir M. P., Kornberg T. RT Patterns of engrailed and fushi tarazu transcripts reveal novel intermediate stages in Drosophila segmentation RL Nature 318:433-439 (1985). RN [16]; RE0005038. RX PUBMED: 2563673. RA Han K., Levine M. S., Manley J. L. RT Synergistic activation and repression of transcription by Drosophila homeobox proteins RL Cell 56:573-583 (1989). RN [17]; RE0005039. RX PUBMED: 1861720. RA Heemskerk J., DiNardo S., Kostriken R., Farrell P. H. RT Multiple modes of engrailed regulation in the progression towards cell fate determination RL Nature 352:404-410 (1991). RN [18]; RE0005040. RX PUBMED: 3123316. RA DiNardo S., Farrell P. H. RT Establishment and refinement of segmental pattern in the Drosophila embryo: spatial control of engrailed expression by pair rule genes RL Genes Dev. 1:1212-1225 (1987). RN [19]; RE0005041. RX PUBMED: 3955654. RA Howard K., Ingham P. RT Regulatory interactions between the segmentation genes fushi tarazu, hairy and engrailed in the Drosophila blastoderm RL Cell 44:949-957 (1986). RN [20]; RE0005042. RX PUBMED: 1335365. RA Siegfried E., Chou T. -B., Perrimon N. RT wingless siganlling acts through zeste-white 3, the Drosophila homolog of glycogen synthase kinase-3, to regulate engrailed and establish cell fate RL Cell 71:1167-1179 (1992). RN [21]; RE0005043. RX PUBMED: 2481829. RA Fjose A., McGinnis W., Gehring W. J. RT Isolation of a homoeo box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts RL Nature 313:284-289 (1985). RN [22]; RE0015328. RX PUBMED: 7902124. RA Kalionis B., O'Farrell P. H. RT A universal target sequence is bound in vitro by diverse homeodomains RL Mech. Dev. 43:57-70 (1993). XX //