AC T01062
XX
ID T01062
XX
DT 24.02.1994 (created); ewi.
DT 02.07.2014 (updated); sla.
CO Copyright (C), QIAGEN.
XX
FA AML3
XX
SY AML3; CBF-alpha 1; CBFA1; PEBP2alpha1; PEBP2alphaA; PEBP2alphaA1; Runt-related transcription factor 2; RUNX2.
XX
OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
XX
GE G005054 Runx2.
XX
CL C0029; runt.
XX
SZ 513 AA; 55.7 kDa (cDNA) (calc.).
XX
SQ MRIPVDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQQQQ
SQ QQEAAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPS
SQ HWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFV
SQ GRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSD
SQ LGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLS
SQ QMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSSLSKKSQAGASELG
SQ PFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPG
SQ SSQSQSGPFQTSSTPYLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLLNPNLP
SQ NQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY
XX
SC Swiss-Prot#Q08775-3
XX
FT 2 232 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 91 225 
PF00853; Runt domain.
FT 93 221 
PS51062; RUNT.
FT 97 97 
I 97 T leads to stronger DNA-binding [5].
FT 115 115 
C is protected by beta subunit against oxidational attack [4].
FT 124 124 
C 124 S leads to stronger DNA-binding [5].
FT 124 124 
C is protected by beta subunit against oxidational attack [4].
FT 133 133 
K 133 N leads to decreased expression [5].
FT 139 139 
E 139 V leads to decreased expression [5].
FT 149 149 
M 149 V abolish binding to beta subunit [5].
FT 150 150 A 150 T abolish DNA-binding and binding to beta subunit [5].
FT 151 151 G 151 R abolish binding to beta subunit [5].
FT 152 152 N 152 D abolish binding to beta subunit [5].
FT 183 183 S 183 G abolish DNA- binding and binding to beta subunit [5].
FT 388 414 putative nuclear matrix targeting signal [6].
XX
SF DNA-binding and heterodimerization;
SF both runt domain and C-terminal region are influencing the nuclear accumulation [3];
XX
FF AML3 drives mesenchymal stem cells to differentiate into osteoblasts [8];
FF DNA-binding;
FF heterodimer: increased affinity;
FF expression is tissue type dependent [2];
FF believed to be involved in T-cell-specific gene expression [2];
XX
IN T00111 c-Ets-1; mouse, Mus musculus.
IN T01065 PEBP2beta-isoform2; mouse, Mus musculus.
IN T22859 TAZ; mouse, Mus musculus.
XX
MX M07276 V$AML3_Q3.
MX M08866 V$AML_Q4.
MX M00769 V$AML_Q6.
MX M00731 V$OSF2_Q6.
MX M00984 V$PEBP_Q6.
XX
BS R04450.
BS R01431.
BS R01222.
BS R01228.
XX
DR TRANSPATH: MO000025371.
DR EMBL: D14636;
DR UniProtKB: Q08775-3; RUNX2_MOUSE.
XX
RN [1]; RE0002540.
RX PUBMED: 8341710.
RA Ogawa E., Maruyama M., Kagoshima H., Inuzuka M., Lu J., Satake M., Shigesada K., Ito Y.
RT PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene
RL Proc. Natl. Acad. Sci. USA 90:6859-6863 (1993).
RN [2]; RE0006071.
RX PUBMED: 7862157.
RA Satake M., Nomura S., Yamaguchi-Iwai Y., Takahama Y., Hashimoto Y., Niki M., Kitamura Y., Ito Y.
RT Expression of the Runt domain-encoding PEBP2alpha genes in T cells during thymic development
RL Mol. Cell. Biol. 15:1662-1670 (1995).
RN [3]; RE0006088.
RX PUBMED: 7862156.
RA Lu J., Maruyama M., Satake M., Bae S., Ogawa E., Kagoshima H., Shigesada K., Ito Y.
RT Subcellular localization of the alpha and beta subunits of the acute myeloid leukemia-linked transcription factor PEBP2/CBF
RL Mol. Cell. Biol. 15:1651-1661 (1995).
RN [4]; RE0006207.
RX PUBMED: 9169404.
RA Akamatsu Y., Ohno T., Hirota K., Kagoshima H., Yodoi J., Shigesada K.
RT Redox regulation of the DNA binding activity in transcription factor PEBP2
RL J. Biol. Chem. 272:14497-14500 (1997).
RN [5]; RE0006212.
RX PUBMED: 9034321.
RA Akamatsu Y., Tsukumo S., Kagoshima H., Tsurushita N., Shigesada K.
RT A simple screening for mutant DNA binding proteins: application to murine transcription factor PEBP2alpha subunit, a founding member of the Runt domain protein family
RL Gene 185:111-117 (1997).
RN [6]; RE0006488.
RX PUBMED: 9192636.
RA Zeng C., van Wijnen A. J., Stein J. L., Meyers S., Sun W., Shopland L., Lawrence J. B., Penman S., Lian J.B., Stein G. S., Hiebert S. W.
RT Identification of a nuclear matrix targeting signal in leukemia and bone-related AML/CBF-alpha transcription factors
RL Proc. Natl. Acad. Sci. USA 94:6746-6751 (1997).
RN [7]; RE0016625.
RX PUBMED: 7774816.
RA Giese K., Kingsley C., Kirshner J. R., Grosschedl R.
RT Assembly and function of a TCR alpha enhancer complex id dependent on LEF-1-induced DNA bending and multiple protein-protein interactions.
RL Genes Dev. 9:995-1008 (1995).
RN [8]; RE0035398.
RX PUBMED: 16099986.
RA Hong J. H., Hwang E. S., McManus M. T., Amsterdam A., Tian Y., Kalmukova R., Mueller E., Benjamin T., Spiegelman B. M., Sharp P. A., Hopkins N., Yaffe M. B.
RT TAZ, a transcriptional modulator of mesenchymal stem cell differentiation.
RL Science 309:1074-1078 (2005).
XX
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