AC T02158
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ID T02158
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DT 26.05.1997 (created); hiwi.
DT 25.11.2006 (updated); vma.
CO Copyright (C), QIAGEN.
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FA TFIIB
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SY alpha (rat); CB; CG5193; CI; CT16633; dTFIIB; FA; factor e; TFIIB; Transcription factor IIB.
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OS fruit fly, Drosophila melanogaster
OC eukaryota; animalia; metazoa; arthropoda; insecta; diptera; drosophiloidea; drosophilidae
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GE G014995 TfIIB.
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CL C0030; histone fold.
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SZ 315 AA; 34.4 kDa (cDNA) (calc.), 38 kDa (SDS) [4]
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SQ MASTSRLDNNKVCCYAHPESPLIEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNEKSG
SQ VDPSRVGGPENPLLSGGDLSTIIGPGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEI
SQ SSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEIC
SQ AVSKISKKEIGRCFKLTLKALETSVDLITTADFMCRFCANLDLPNMVQRAATHIAKKAVE
SQ MDIVPGRSPISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFP
SQ EDFKFTTPIDQLPQM
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SC Swiss-Prot#P29052
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FT 21 105 
required for basal transcription in addition to TBP-TATA-binding [7].
FT 105 315 
binding to TBP-TATA complexes [7].
FT 118 199 
SM00385; cyclin_7.
FT 120 190 
PF00382; Transcription factor TFIIB repeat.
FT 179 190 
homology to sigma2.1 region [4].
FT 184 199 
cluster of basic residues, crucial for complex formation with TBP and TATA box [7].
FT 189 208 
homology to sigma2.4 region [4].
FT 205 219 
homology to sigma2.2 region [4].
FT 212 293 
SM00385; cyclin_7.
FT 214 284 
PF00382; Transcription factor TFIIB repeat.
FT 225 239 homology to sigma2.2 region [4].
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SF mediates trans-activation by glutamine-rich activation domains, probably through a zinc-finger like structure near the N-terminus [3] [6];
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FF may be involved in fixing the transcription start site [5];
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IN T02125 TAFII40; fruit fly, Drosophila melanogaster.
IN T00797 TBP; fruit fly, Drosophila melanogaster.
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DR TRANSPATH: MO000045990.
DR EMBL: M88164; DMTFIIBA.
DR EMBL: M91081; DMTFIIB.
DR EMBL: U02879; DMFIIB.
DR EMBL: U35148; DM35148.
DR UniProtKB: P29052; TF2B_DROME.
DR FLYBASE: FBgn0004915; TfIIB.
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RN [1]; RE0005533.
RX PUBMED: 7875589.
RA Lira-De Vito L. M., Burke T. W., Kadonaga J. T.
RT Structure of the genes encoding transcription factor IIB and TATA box- binding protein from Drosophila melanogaster.
RL Gene 153:203-207 (1995).
RN [2]; RE0005607.
RX PUBMED: 8221891.
RA Goodrich J. A., Hoey T., Thut C. J., Admon A., Tjian R.
RT Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB
RL Cell 75:519-530 (1993).
RN [3]; RE0005677.
RX PUBMED: 7891725.
RA Colgan J., Ashali H., Manley J. L.
RT A direct interaction betwenn a glutamine-rich activato and the N terminus of TFIIB can mediate transcriptional activation in vivo
RL Mol. Cell. Biol. 15:2311-2320 (1995).
RN [4]; RE0005684.
RX PUBMED: 1557390.
RA Yamashita S., Wada K., Horikoshi M., Gong D.-W., Kokubo T., Hisatake K., Yokotani N., Malik S., Roeder R. G., Nakatani Y.
RT Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB
RL Proc. Natl. Acad. Sci. USA 89:2839-2843 (1992).
RN [5]; RE0005685.
RX PUBMED: 1644295.
RA Wampler S. L., Kadonaga J. T.
RT Functional analysis of Drosophila transcription factor IIB
RL Genes Dev. 6:1542-1552 (1992).
RN [6]; RE0005694.
RX PUBMED: 8464496.
RA Colgan J., Wampler S., Manley J. L.
RT Interaction between a transcriptional activator and transcription factor IIB in vivo
RL Nature 362:549-553 (1993).
RN [7]; RE0005696.
RX PUBMED: 8332911.
RA Yamashita S., Hisatake K., Kokubo T., Doi K., Roeder R. G., Horikoshi M., Nakatani Y.
RT Transcription factor TFIIB sites important for interaction with promoter-bound TFIID
RL Science 261:463-466 (1993).
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