AC T00815
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ID T00815
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DT 15.10.1992 (created); ewi.
DT 10.12.2004 (updated); cch.
CO Copyright (C), QIAGEN.
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FA TFIIA
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OS yeast, Saccharomyces cerevisiae
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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SF heterodimer of a 32 and a 13.5 kDa subunit, TOA1 T02227 and TOA2 T02228, respectively [7];
SF TFIIA binds to preformed TFIID-TATA complexes with higher affinity (D = 20 pM) than to TFIID, or than TFIID binds to the TATA element [7];
SF TFIIA possesses a chymotrypsin-resistant core comprising N- and C-terminal peptides of TOA1 and the entire TOA2 peptide chain [5];
SF co-crystals of this core, TBP and a TATA-box DNA: boot-like structure of the TFIIA moiety, the "leg" being formed by a 12-stranded beta-barrel, the "foot" by a four helix bundle [5];
SF the top of the boot interacts with the upstream part of TBP and with the DNA [5];
SF TFIIA thus provides the upstream interface of the basal transcription complex which may be contacted by certain activators [5];
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FF mediates the effect by certain but not all upstream activators by changing the DNA conformation such that they can contact the transcription initiation complex [4] [3];
FF enhances the affinity of TBP to bind to DNA [9];
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IN T00798 Spt15p; yeast, Saccharomyces cerevisiae.
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BS R01015.
BS R01016.
BS R00970.
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DR PDB: 1ytf.
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RN [1]; RE0000039.
RX PUBMED: 2917366.
RA Buratowski S., Hahn S., Guarente L., Sharp P. A.
RT Five Intermediate Complexes in Transcription Initiation by RNA Polymerase II
RL Cell 56:549-561 (1989).
RN [2]; RE0000218.
RX PUBMED: 1586948.
RA Schultz M. C., Reeder R. H., Hahn S.
RT Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters
RL Cell 69:697-702 (1992).
RN [3]; RE0000439.
RX PUBMED: 2684641.
RA Hahn S., Buratowski S., Sharp P. A., Guarente L.
RT Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA
RL EMBO J. 8:3379-3382 (1989).
RN [4]; RE0005516.
RX PUBMED: 7604282.
RA Stargell L. A., Struhl K.
RT The TBP-TFII interaction in the response to acidic activators in vivo
RL Science 269:75-78 (1995).
RN [5]; RE0005517.
RX PUBMED: 8610010.
RA Tan S., Hunziker Y., Sargent D. F., Richmond T. J.
RT Crystal structure of a yeast TFIIA/TBP/DNA complex
RL Nature 381:127-134 (1996).
RN [6]; RE0005668.
RX PUBMED: 1546313.
RA Ranish J. A., Lane W. S., Hahn S.
RT Isolation of two genes that encode subunits of the yeast transcription factor IIA
RL Science 255:1127-1129 (1992).
RN [7]; RE0005669.
RX PUBMED: 1918049.
RA Ranish J. A., Hahn S.
RT The yeast general transcription factor TFIIA is composed of two polypeptide subunits
RL J. Biol. Chem. 266:19320-19327 (1991).
RN [8]; RE0005670.
RX PUBMED: 8091863.
RA Rasmussen S. W.
RT Sequence of a 28.6 kb Region of Yeast Chromosome XI Includes the FAB1 and TOA2 Genes, an Open Reading Frame (ORF) Similar to a Translationally Controlled Tumor Protein, one ORF Containing Motifs also Found in Plant Storage Proteins.
RL Yeast 10:69-74 (1994).
RN [9]; RE0005671.
RX PUBMED: 8132551.
RA Imbalzano A. N., Zaret K. S., Kingston R. E.
RT Transcription factor (TF) IIB and TFIIA can independently increase the affinity of the TATA-binding protein for DNA
RL J. Biol. Chem. 269:8280-8286 (1994).
RN [10]; RE0005672.
RX PUBMED: 7862117.
RA Kang J. J., Auble D. T., Ranish J. A., Hahn S.
RT Analysis of the yeast transcription factor TFIIA: Distinct functional regions and a polymerase II-specific role in basal and activated transcription
RL Mol. Cell. Biol. 15:1234-1243 (1995).
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