AC T00819
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ID T00819
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DT 15.10.1992 (created); ewi.
DT 29.11.2012 (updated); mkl.
CO Copyright (C), QIAGEN.
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FA Sua7p
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SY factor e; SUA7; SUA7p; TFIIB; YPR086W.
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OS yeast, Saccharomyces cerevisiae
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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GE G004182 SUA7.
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SZ 345 AA; 38.2 kDa (cDNA) (calc.), 41 kDa (SDS)
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SQ MMTRESIDKRAGRRGPNLNIVLTCPECKVYPPKIVERFSEGDVVCALCGLVLSDKLVDTR
SQ SEWRTFSNDDHNGDDPSRVGEASNPLLDGNNLSTRIGKGETTDMRFTKELNKAQGKNVMD
SQ KKDNEVQAAFAKITMLCDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGC
SQ RRAEVARTFKEIQSLIHVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIP
SQ RFCSHLGLPMQVTTSAEYTAKKCKEIKEIAGKSPITIAVVSIYLNILLFQIPITAAKVGQ
SQ TLQVTEGTIKSGYKILYEHRDKLVDPQLIANGVVSLDNLPGVEKK
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SC Swiss-Prot#P29055
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FT 69 335 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 131 212 
SM00385; cyclin_7.
FT 133 203 
PF00382; Transcription factor TFIIB repeat.
FT 237 318 
SM00385; cyclin_7.
FT 239 309 
PF00382; Transcription factor TFIIB repeat.
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SF 58% and 65% similarity of the two imperfect repeats with those of human TFIIB [3];
SF pI(calc.) = 8.64 [3];
SF mutations in positions 62 and 78 can be suppressed by mutations in SUB1 (encoding a PC4-homologous co-activator), in SSU71 (encoding yeast RAP74/TFIIF-alpha), or in SSU73 (encoding the 14.3-kDa subunit of pol II) [4] [6] [9];
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FF functions in selecting the proper transcription start site [3];
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IN T02137 PC4; human, Homo sapiens.
IN T00798 Spt15p; yeast, Saccharomyces cerevisiae.
IN T00794 TBP; human, Homo sapiens.
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BS R01016.
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DR EMBL: M81380; SCSUA7A.
DR UniProtKB: P29055; TF2B_YEAST.
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RN [1]; RE0000039.
RX PUBMED: 2917366.
RA Buratowski S., Hahn S., Guarente L., Sharp P. A.
RT Five Intermediate Complexes in Transcription Initiation by RNA Polymerase II
RL Cell 56:549-561 (1989).
RN [2]; RE0005534.
RX PUBMED: 2187859.
RA Amaya Y., Nakano A., Ito K., Mori M.
RT Isolation of a yeast gene, SRH1, that encodes a homologue of the 54K subunit of mammalian signal recognition particle
RL J. Biochem. 107:457-463 (1990).
RN [3]; RE0005692.
RX PUBMED: 1547497.
RA Pinto I., Ware D. E., Hampsey M.
RT The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo
RL Cell 68:977-988 (1992).
RN [4]; RE0005704.
RX PUBMED: 8617240.
RA Knaus R., Pollock R., Guarente L.
RT Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4
RL EMBO J. 15:1933-1940 (1996).
RN [5]; RE0005705.
RX PUBMED: 8657130.
RA Sun Z.-W., Hampsey M.
RT Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo
RL Mol. Cell. Biol. 16:1557-1566 (1996).
RN [6]; RE0005706.
RX PUBMED: 7724527.
RA Sun Z.-W., Hampsey M.
RT Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae
RL Proc. Natl. Acad. Sci. USA 92:3127-3131 (1995).
RN [7]; RE0005707.
RX PUBMED: 1454810.
RA Tschochner H., Sayre M. H., Flanagan P. M., Feaver W. J., Kornberg R. D.
RT Yeast RNA polymerase II initiation factor e: Isolation and identification as the functional counterpart of human transcription factor IIB
RL Proc. Natl. Acad. Sci. USA 89:11292-11296 (1992).
RN [8]; RE0005708.
RX PUBMED: 7982976.
RA Pinto I., Wu W.-H., Na J. G., Hampsey M.
RT Characterization of sua7 mutations defines a domain of TFIIB involved in transcription start site selection in yeast
RL J. Biol. Chem. 269:30569-30573 (1994).
RN [9]; RE0005709.
RX PUBMED: 8692696.
RA Sun Z.-W., Tessmer A., Hampsey M.
RT Functional interaction between TFIIB and the Rpb9 (Ssu73) subunit of RNA polymerase II in Saccharomyces cerevisiae
RL Nucleic Acids Res. 24:2560-2566 (1996).
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