AC T02924
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ID T02924
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DT 31.01.2000 (created); sur.
DT 14.07.2005 (updated); ili.
CO Copyright (C), QIAGEN.
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FA TCF-B
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SY Pan; Pangolin; TCF-B.
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OS fruit fly, Drosophila melanogaster
OC eukaryota; animalia; metazoa; arthropoda; insecta; diptera; drosophiloidea; drosophilidae
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GE G018680 pan.
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HO LEF-1.
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CL C0015; HMG.
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SZ 743 AA; 81.0 kDa (cDNA) (calc.).
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SQ MPHTHSRHGSSGDDLCSTDEVKIFKDEGDREDEKISSENLLVEEKSSLIDLTESEEKGHK
SQ ISRPDHSPVFNKLDTHAPSFNMGYLVSPYSYANGSPSGLPVTMANKIGLPPFFCHNADPL
SQ STPPPAHCGIPPYQLDPKMGLTRPALYPFAGGQYPYPMLSSDMSQVASWHTPSVYSASSF
SQ RTPYPSSLPINTTLASDFPFRFSPSLLPSVHATSHHVINAHSAIVGVSSKQECGVQDPTT
SQ NNRYPRNLEAKHTSNAQSNESKETTNDKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKES
SQ AAINQILGRRWHALGREEQAKYYELARRERQLHMQMYPDWSSRTNASRGKKRKRKQDTND
SQ GKKCRARFGLDQQSQWCKPCRRKKKCIRYMEALNGNGPAEDGSCFDEHGSQLSDDDEDDY
SQ DDDKLGGSCGSADETNKIEDEDSESLNQSMPSPGCLSGLSSLQSPSTTMSLASPLNMNAN
SQ SATNVIFPASSNALLIVGADQPTAQQRPTLVSTSGSSSGSTSSISTTPNTSSTVSPVTCM
SQ TGPCLGSSQERAMMLGNRFSHLGMGLSPPVVSTSTSKSEPFFKPHPTVCNNPIFALPSIG
SQ NCSLNISSMPNTSRNPIGANPRDINNPLSINQLTKRREYKNVELIEASESKTIVAHAATS
SQ IIQHVAVNGYHANHSLLNSNLGHLHHQLNNRTENPNRSEQTMLSVSNHSVNSSECHKESD
SQ SQAIVSSNPPNAGSSDNGVISVS
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SC taken from [2]
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FT 25 25 
Lys 25 is acetylated by dCBP [4].
FT 268 722 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 272 342 
SM00398; hmgende2.
FT 273 341 
PF00505; HMG (high mobility group) box.
FT 273 341 
PS50118; HMG_BOX_2.
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SF 2 splice variants A and B;
SF http://fly.ebi.ac.uk:7081/.bin/fbidq.html?FBgn0019664>;
SF dTCF binds to a canonical DNA motif and interacts with Armadillo (beta-catenin homolog) [2];
SF closely related to mammalian TCF-1;
SF Pangolin (TCF) is a single-copy gene which spans approx. 50 kb on chromosome 4;
SF it is composed of 13 exons (I, II, III, IV, V, VI, VII, VIII, IX, X, XIA, XIB, XII, XIII);
SF Pangolin seems to be more similar to mammalian TCF-1 than to mammalian LEF-1 [5];
SF mutation of TCF-binding sites in TCF target gene promoters reduces the level of gene expression;
SF as these mutations also result in ectopic gene expression outside the normal domains it is supposed that TCF can function as either an activator or repressor of gene expression [3];
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CP adults.
CN not expressed during embryogenesis [2].
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FF TCFs can either act as activators or repressors of Wingless (Wg) - responsive genes depending on the state of the Wg signaling pathway;
FF Armadillo (the Drosophila homologue of beta-catenin) is the coactivator and Groucho is the corepressor [3];
FF interacts with Drosophila CREB-binding protein (dCBP);
FF dCBP contains a HAT (histone acetyl transferase) domain which acetylates a conserved lysine (Lys 25) in the Armadillo-binding domain of TCF and thus lowers the affinity of Armadillo binding to TCF;
FF thus dCBP acts as an repressor of TCF;
FF the HMG domain of TCF is sufficient to bind to dCBP [4];
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IN T02977 armadillo; fruit fly, Drosophila melanogaster.
IN T03236 CBP; fruit fly, Drosophila melanogaster.
IN T03224 Grg-5; clawed frog, Xenopus laevis.
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DR TRANSPATH: MO000026887.
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RN [1]; RE0006122.
RX PUBMED: 9039917.
RA Brunner E., Peter O., Schweizer L., Basler K.
RT Pangolin encodes a Lef-1 homologue that acts downstream of Armadillo to transduce the Wingless signal in Drosophila
RL Nature 385:829-833 (1997).
RN [2]; RE0014038.
RX PUBMED: 9118222.
RA van der Wetering M., Cavallo R., Dooijes D., van Beest M., van Es J., Loureiro J., Ypma A., Hursh D., Jones T., Bejsovec A., Peifer M., Mortin M., Clevers H.
RT Armadillo coactivates transcription driven by the product of the drosophila segment polarity gene dTCF
RL Cell 88:789-799 (1997).
RN [3]; RE0014219.
RX PUBMED: 9783586.
RA Cavallo R. A., Cox R. T., Moline M. M., Roose J., Polevoy G. A., Clevers H., Peifer M., Bejsovec A.
RT Drosophila Tcf and Groucho interact to repress Wingless signalling activity
RL Nature 395:604-608 (1998).
RN [4]; RE0014226.
RX PUBMED: 9774110.
RA Waltzer L., Bienz M.
RT Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling
RL Nature 395:521-525 (1998).
RN [5]; RE0014335.
RX PUBMED: 9613571.
RA Dooijes D., van Beest M., van de Wetering M., Boulanger G., Jones T., Clevers H., Mortin M. A.
RT Genomic organization of the segment polarity gene pan in Drosophila melanogaster
RL Mol. Gen. Genet. 258:45-52 (1998).
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