AC T04370
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ID T04370
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DT 06.03.2001 (created); rio.
DT 22.10.2012 (updated); spk.
CO Copyright (C), QIAGEN.
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FA Hand2
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SY dHand; heart- and neural crest derivatives-expressed 2; Hed; Th2; Thing2.
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OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
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GE G005386 Hand2.
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CL C0010; bHLH; 1.2.3.2.4.
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SF bHLH region is 87% identical to that of Hand1 T01569 [2];
SF Hand2 -/- mice develop no second branchial arch, show severe cardiac failures (lack right ventricle, poorly trabeculated left ventricle), have a disorganized vasculature (vascular mesenchyme fails to differentiate into vascular smooth muscle cells) and die after day 10.5 [4] [8];
SF Hand1 T01569 and Hand2 show a complementary asymmetrical expression in the developing left and right heart chamber respectively [4];
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CP walls of dorsal aorta and aortic arch arteries, yolk sac vessels, (E10.0:) mesenchyme underlying the branchial arch epithelium, aorta, aortic arch, posterior limb bud mesoderm, (E10.5:) 1. branchial arches, truncus arteriosus, aortic sac, vascular mesenchyme between 3./4. aortic arch arteries, limb buds, (E13.5:) neural crest-derived sympathetic trunk, adrenal medulla, (E13.0-16.0:) interdigit area, (adult:) aorta, (cell lines:) vascular cell line A-10 [5] [8] [9] [2].
CN F9 embryonal carcinoma cells [3].
EX adrenal medulla,,,Theiler Stage 21; medium; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX anterior cardinal vein,,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX anterior cardinal vein,,,Theiler Stage 17; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX aortic arch,,,Theiler Stage 16; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX aortic sac,,,Theiler Stage 13; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX aortic sac,,,Theiler Stage 17; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX atrial endocardium,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX atrial endocardium,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX atrial myocardium,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX atrial myocardium,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX atrium of heart (right and left),,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX atrium of heart (right and left),,,Theiler Stage 17; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX brain,,,adult; none; Northern blot; mRNA (poly-A); [3].
EX brain,,,adult; none; Northern blot; total RNA; [3].
EX bulbus cordis,,,Theiler Stage 13; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX common cardinal vein,,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX common cardinal vein,,,Theiler Stage 17; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX compact myocardial outer layer of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX decidua,,,Theiler Stage 11; high; Northern blot; total RNA; [3].
EX decidua,,,Theiler Stage 11; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX endocardial cushion tissue,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial tissue of left part of ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial tissue of left part of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial tissue of outflow tract,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial tissue of outflow tract,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial tissue of right part of ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial tissue of right part of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardium of atrio-ventricular canal,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardium of atrio-ventricular canal,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardium of inflow tract of heart,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardium of primitive heart tube,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardium of ventriculoinfundibular fold,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX extraembryonic component,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX extraembryonic component,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX first aortic arch,,,Theiler Stage 15; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX first pharyngeal arch,,,Theiler Stage 17; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX fourth aortic arch,,,Theiler Stage 17; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX future brain,,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX heart,,,Theiler Stage 13; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX heart,,,Theiler Stage 21; very low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX heart,,,adult; high; Northern blot; mRNA (poly-A); [3].
EX intestine,,,adult; none; Northern blot; total RNA; [3].
EX kidney (right and left),,,adult; none; Northern blot; mRNA (poly-A); [3].
EX kidney (right and left),,,adult; none; Northern blot; total RNA; [3].
EX lateral plate mesoderm,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX liver,,,adult; medium; Northern blot; mRNA (poly-A); [3].
EX liver,,,adult; none; Northern blot; total RNA; [3].
EX lung,,,adult; none; Northern blot; mRNA (poly-A); [3].
EX lung,,,adult; none; Northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler Stage 11; none; Northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler Stage 11; none; Northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX mouse, Mus musculus,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX mouse, Mus musculus,,,Theiler Stage 13; detectable; Northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler Stage 15; detectable; Northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler Stage 17; detectable; Northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler Stage 24; none; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX mouse, Mus musculus,,,Theiler Stage 25; none; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX mouse, Mus musculus,,,Theiler Stage 26; none; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX mouse, Mus musculus,undifferentiated embryonic stem cell,,undefined; none; Northern blot; RNA (undefined); [3].
EX muscles,,,adult; none; Northern blot; mRNA (poly-A); [3].
EX myocardium of atrio-ventricular canal,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of atrio-ventricular canal,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of inflow tract,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of left part of ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of left part of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of outflow tract,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of outflow tract,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of primitive heart tube,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of right part of ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX myocardium of right part of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX outflow tract of heart,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX outflow tract of heart,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX outflow tract of heart,,,Theiler Stage 15; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX ovary,,,adult; none; Northern blot; total RNA; [3].
EX pharyngeal arches,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX placenta,,,Theiler Stage 11; none; Northern blot; total RNA; [3].
EX placenta,,,Theiler Stage 13; none; Northern blot; total RNA; [3].
EX placenta,,,Theiler Stage 15; none; Northern blot; total RNA; [3].
EX placenta,,,Theiler Stage 17; none; Northern blot; total RNA; [3].
EX posterior cardinal vein,,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX posterior cardinal vein,,,Theiler Stage 17; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX precardiogenic mesoderm,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive heart tube,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive heart tube,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive ventricle (future left ventricle),,,Theiler Stage 13; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX right ventricle,,,Theiler Stage 16; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX second aortic arch,,,Theiler Stage 15; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX somatopleure,,,Theiler Stage 13; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX splanchnopleure,,,Theiler Stage 13; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX spleen,,,adult; none; Northern blot; mRNA (poly-A); [3].
EX sympathetic trunk,,,Theiler Stage 21; medium; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX testis (right and left),,,adult; low; Northern blot; mRNA (poly-A); [3].
EX third aortic arch,,,Theiler Stage 17; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX truncus arteriosus,,,Theiler Stage 15; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX truncus arteriosus,,,Theiler Stage 17; high; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX uterus,,,adult; none; Northern blot; total RNA; [3].
EX venous sinus of primitive heart,,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX venous sinus of primitive heart,,,Theiler Stage 17; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX ventricle of heart (right and left),,,Theiler Stage 15; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX ventricle of heart (right and left),,,Theiler Stage 17; low; RNA-in situ hybridization (not further specified); RNA (undefined); [2].
EX ventricular myocardium at the outer curvature,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
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FF role in right chamber-specific cell fate decisions and possibly involved in cardiac looping [4];
FF part of signaling cascade of branchial arch development: Edn1 (endothelin-1) secretion stimulates Hand2 expression which regulates Msx1 T02072 expression in growing, distal brachial arches [5];
FF involved in development of segments of the heart tube which give rise to specific chambers of the heart during cardiogenesis [6];
FF expression of Ufd1 (ubiquitin fusion degradation protein 1) and VEGF165 receptor (neuropilin-1) is dependent on Hand2 [7] [8];
FF required for vascular development, regulates angiogenesis possibly through a VEGF (vascular endothelial growth factor) signaling pathway [8];
FF role in anterior-posterior patterning of the limb bud [9];
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IN T09638 Hand2; mouse, Mus musculus.
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MX M01034 V$EBOX_Q6_01.
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DR TRANSPATH: MO000028227.
DR UniProtKB: Q61039;
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RN [1]; RE0006047.
RX PUBMED: 9192865.
RA Biben C., Harvey R. P.
RT Homeodomain factor Nkx2-5 controls left/right asymmetric expression of bHLH gene eHand during murine heart development
RL Genes Dev. 11:1357-1369 (1997).
RN [2]; RE0006279.
RX PUBMED: 8533092.
RA Srivastava D., Cserjesi P., Olson E. N.
RT A subclass of bHLH proteins required for cardiac morphogenesis
RL Science 270:1995-1999 (1995).
RN [3]; RE0015952.
RX PUBMED: 7671815.
RA Cross J. C., Flannery M. L., Blanar M. A., Steingrimsson E., Jenkins N. A., Copeland N. G., Rutter W. J., Werb Z.
RT Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development
RL Development 121:2513-2523 (1995).
RN [4]; RE0015953.
RX PUBMED: 9171826.
RA Srivastava D., Thomas T., Lin Q., Kirby M. L., Brown D., Olson E. N.
RT Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND
RL Nat. Genet. 16:154-160 (1997).
RN [5]; RE0015954.
RX PUBMED: 9671575.
RA Thomas T., Kurihara H., Yamagishi H., Kurihara Y., Yazaki Y., Olson E. N., Srivastava D.
RT A signaling cascade involving endothelin-1, dHAND and Msx1 regulates development of neural-crest-derived branchial arch mesenchyme
RL Development 125:3005-3014 (1998).
RN [6]; RE0015956.
RX PUBMED: 9576835.
RA Thomas T., Yamagishi H., Overbeek P. A., Olson E. N., Srivastava D.
RT The bHLH factors, dHAND and eHAND, specify pulmonary and systemic cardiac ventricles independent of left-right sidedness
RL Dev. Biol. 196:228-236 (1998).
RN [7]; RE0015961.
RX PUBMED: 10024240.
RA Yamagishi H., Garg V., Matsuoka R., Thomas T., Srivastava D.
RT A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects
RL Science 283:1158-1161 (1999).
RN [8]; RE0015975.
RX PUBMED: 10675351.
RA Yamagishi H., Olson E. N., Srivastava D.
RT The basic helix-loop-helix transcription factor, dHAND, is required for vascular development.
RL J. Clin. Invest. 105:261-270 (2000).
RN [9]; RE0015976.
RX PUBMED: 10769237.
RA Fernandez-Teran M., Piedra M. E., Kathiriya I. S., Srivastava D., Rodriguez-Rey J. C., Ros M. A.
RT Role of dHAND in the anterior-posterior polarization of the limb bud: implications for the Sonic hedgehog pathway
RL Development 127:2133-2142 (2000).
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