AC T01569
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ID T01569
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DT 17.10.1995 (created); ewi.
DT 14.10.2009 (updated); jig.
CO Copyright (C), QIAGEN.
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FA EHAND
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SY eHand; heart- and neural crest derivatives-expressed 1; Hxt; Th1; Thing1.
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OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
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GE G005385 Hand1.
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CL C0010; bHLH; 1.2.3.2.3.
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SF bHLH region is 87% identical to that of Hand2 T04370 [3];
SF no homodimerization, DNA-binding depends on heterodimerization [1];
SF the EHAND-bound half-sites are highly degenerate [1];
SF EHAND and Hand2 T04370 show a complementary asymmetrical expression in the developing left and right heart chamber respectively [5];
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CP (E10.5:) portions of the heart, autonomic ganglia, gut, pharyngeal arches; (adult:) intestine, lung, placenta [1].
CN brain, heart, kidney, lung, skeletal muscle, spleen [1]; F9 embryonal carcinoma cells [4] [4] [1].
EX adrenal medulla,,,Theiler Stage 21; medium; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX allantois,,,Theiler Stage 15; none; Northern blot; RNA (undefined); [4].
EX allantois,,,Theiler Stage 15; none; Northern blot; RNA (undefined); [4].
EX allantois,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX allantois,extraembryonic mesoderm cell,,Theiler Stage 11; low; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX amniotic ectoderm,extraembryonic mesoderm cell,,Theiler Stage 11; low; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX atrial endocardium,,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrial endocardium,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrial myocardium,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrial myocardium,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrio-ventricular canal,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrio-ventricular canal,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrium of heart (right and left),,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX atrium of heart (right and left),,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX blastocyst,,,Theiler Stage 4; detectable; RT-PCR; RNA (undefined); [4].
EX brain,,,adult; high; Northern blot; mRNA (poly-A); [4].
EX brain,,,adult; none; Northern blot; total RNA; [4].
EX caudal region of heart tube,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX chorion,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX chorion,,,Theiler Stage 15; none; Northern blot; RNA (undefined); [4].
EX chorion,,,Theiler Stage 15; none; Northern blot; RNA (undefined); [4].
EX chorion,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX chorionic ectoderm,extraembryonic mesoderm cell,,Theiler Stage 11; low; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX compact myocardial outer layer of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX decidua,,,Theiler Stage 11; high; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX decidua,,,Theiler Stage 11; none; Northern blot; total RNA; [4].
EX decidua,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX dividing egg,,,Theiler Stage 2; detectable; RT-PCR; RNA (undefined); [4].
EX ectoplacental cone,trophoblast cell,,Theiler Stage 11; high; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX endocardial cushion tissue,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardial tissue of left part of ventricle,,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardial tissue of left part of ventricle,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardial tissue of outflow tract,,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardial tissue of outflow tract,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardial tissue of right part of ventricle,,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardial tissue of right part of ventricle,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardium of atrio-ventricular canal,,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardium of atrio-ventricular canal,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardium of inflow tract of heart,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardium of primitive heart tube,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX endocardium of ventriculoinfundibular fold,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX extraembryonic component,,,Theiler Stage 11; high; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX heart,,,Theiler Stage 21; very low; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX heart,,,adult; none; Northern blot; mRNA (poly-A); [4].
EX intestine,,,adult; none; Northern blot; total RNA; [4].
EX kidney (right and left),,,adult; none; Northern blot; mRNA (poly-A); [4].
EX kidney (right and left),,,adult; none; Northern blot; total RNA; [4].
EX lateral plate mesoderm,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX lateral plate mesoderm,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX left ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX left ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX liver,,,adult; none; Northern blot; mRNA (poly-A); [4].
EX liver,,,adult; none; Northern blot; total RNA; [4].
EX lung,,,adult; none; Northern blot; mRNA (poly-A); [4].
EX lung,,,adult; none; Northern blot; total RNA; [4].
EX morula,,,Theiler Stage 3; detectable; RT-PCR; RNA (undefined); [4].
EX mouse, Mus musculus,,,Theiler Stage 11; high; Northern blot; total RNA; [4].
EX mouse, Mus musculus,,,Theiler Stage 11; none; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX mouse, Mus musculus,,,Theiler Stage 11; very high; Northern blot; total RNA; [4].
EX mouse, Mus musculus,,,Theiler Stage 13; very low; Northern blot; total RNA; [4].
EX mouse, Mus musculus,,,Theiler Stage 14; none; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX mouse, Mus musculus,,,Theiler Stage 15; very low; Northern blot; total RNA; [4].
EX mouse, Mus musculus,,,Theiler Stage 17; very low; Northern blot; total RNA; [4].
EX mouse, Mus musculus,undifferentiated embryonic stem cell,,undefined; none; Northern blot; RNA (undefined); [4].
EX mural trophoectoderm,trophoblast cell,,Theiler Stage 15; high; Northern blot; RNA (undefined); [4].
EX muscles,,,adult; high; Northern blot; mRNA (poly-A); [4].
EX myocardium of atrio-ventricular canal,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of atrio-ventricular canal,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of inflow tract,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of left part of ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of left part of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of outflow tract,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of outflow tract,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of primitive heart tube,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of right part of ventricle,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX myocardium of right part of ventricle,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX one-cell egg,,,Theiler Stage 1; detectable; RT-PCR; RNA (undefined); [4].
EX outflow tract of heart,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX ovary,,,adult; none; Northern blot; total RNA; [4].
EX parietal yolk sac,trophoblast giant cell,,Theiler Stage 11; high; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX pericardium of primitive heart tube,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX pericardium of primitive heart tube,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX pharyngeal arches,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX placenta,,,Theiler Stage 11; high; Northern blot; total RNA; [4].
EX placenta,,,Theiler Stage 13; very high; Northern blot; total RNA; [4].
EX placenta,,,Theiler Stage 15; very high; Northern blot; total RNA; [4].
EX placenta,,,Theiler Stage 17; very high; Northern blot; total RNA; [4].
EX polar trophectoderm,trophoblast cell,,Theiler Stage 15; high; Northern blot; RNA (undefined); [4].
EX precardiogenic mesoderm,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX primitive streak,,,Theiler Stage 10; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive streak,,,Theiler Stage 11; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive streak,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive streak,,,Theiler Stage 13; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive streak,,,Theiler Stage 14; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX primitive streak,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [4].
EX right ventricle,,,Theiler Stage 12; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX right ventricle,,,Theiler Stage 15; none; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX rostral region of heart tube,,,Theiler Stage 12; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
EX spleen,,,adult; none; Northern blot; mRNA (poly-A); [4].
EX sympathetic trunk,,,Theiler Stage 21; medium; RNA-in situ hybridization (not further specified); RNA (undefined); [3].
EX testis (right and left),,,adult; none; Northern blot; mRNA (poly-A); [4].
EX uterus,,,adult; none; Northern blot; total RNA; [4].
EX ventricular myocardium at the outer curvature,,,Theiler Stage 15; detectable; RNA-in situ hybridization (not further specified); RNA (undefined); [5].
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FF activator (as heterodimer with E2A-proteins), may also exert repressor function [1];
FF induces trophoblast formation in preimplantation embryos while Id-1 T00403 inhibits trophoblast differentiation [4];
FF role in left chamber-specific cell fate decisions and possibly involved in cardiac looping [5] [2];
FF Edn1 (endothelin-1) secretion stimulates EHAND expression in the branchial and aortic arches [6];
FF left-sided expression of EHAND but not Hand2 T04370 is under the control of Nkx2-5 T01675 [2];
FF involved in development of segments of the heart tube which give rise to specific chambers of the heart during cardiogenesis [7];
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IN T01034 Da; fruit fly, Drosophila melanogaster.
IN T00204 E12; human, Homo sapiens.
IN T00207 E47; human, Homo sapiens.
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MX M01034 V$EBOX_Q6_01.
MX M00222 V$HAND1E47_01.
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BS R04712.
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DR TRANSPATH: MO000025773.
DR UniProtKB: Q64279;
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RN [1]; RE0003452.
RX PUBMED: 7791788.
RA Hollenberg S. M., Sternglanz R., Cheng P. F., Weintraub H.
RT Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system
RL Mol. Cell. Biol. 15:3813-3822 (1995).
RN [2]; RE0006047.
RX PUBMED: 9192865.
RA Biben C., Harvey R. P.
RT Homeodomain factor Nkx2-5 controls left/right asymmetric expression of bHLH gene eHand during murine heart development
RL Genes Dev. 11:1357-1369 (1997).
RN [3]; RE0006279.
RX PUBMED: 8533092.
RA Srivastava D., Cserjesi P., Olson E. N.
RT A subclass of bHLH proteins required for cardiac morphogenesis
RL Science 270:1995-1999 (1995).
RN [4]; RE0015952.
RX PUBMED: 7671815.
RA Cross J. C., Flannery M. L., Blanar M. A., Steingrimsson E., Jenkins N. A., Copeland N. G., Rutter W. J., Werb Z.
RT Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development
RL Development 121:2513-2523 (1995).
RN [5]; RE0015953.
RX PUBMED: 9171826.
RA Srivastava D., Thomas T., Lin Q., Kirby M. L., Brown D., Olson E. N.
RT Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND
RL Nat. Genet. 16:154-160 (1997).
RN [6]; RE0015954.
RX PUBMED: 9671575.
RA Thomas T., Kurihara H., Yamagishi H., Kurihara Y., Yazaki Y., Olson E. N., Srivastava D.
RT A signaling cascade involving endothelin-1, dHAND and Msx1 regulates development of neural-crest-derived branchial arch mesenchyme
RL Development 125:3005-3014 (1998).
RN [7]; RE0015956.
RX PUBMED: 9576835.
RA Thomas T., Yamagishi H., Overbeek P. A., Olson E. N., Srivastava D.
RT The bHLH factors, dHAND and eHAND, specify pulmonary and systemic cardiac ventricles independent of left-right sidedness
RL Dev. Biol. 196:228-236 (1998).
RN [8]; RE0064387.
RX PUBMED: 15509787.
RA Hill A. A., Riley P. R.
RT Differential regulation of Hand1 homodimer and Hand1-E12 heterodimer activity by the cofactor FHL2.
RL Mol. Cell. Biol. 24:9835-9847 (2004).
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