AC T00761
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ID T00761
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DT 27.01.1993 (created); hse.
DT 16.06.2010 (updated); pro.
CO Copyright (C), QIAGEN.
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FA SRF
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SY Serum Responsive Factor; SRF.
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OS chick, Gallus gallus
OC eukaryota; animalia; metazoa; chordata; vertebrata; aves; neornithes; neognathae; galliformes; phasianidae
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GE G009824 SRF.
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CL C0014; MADS.
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SZ 375 AA; 39.0 kDa (cDNA) (calc.), 39.1 kDa (cDNA) [4]
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SQ PPPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGH
SQ VYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSES
SQ DSSGETKDVLKPTFTVTNLPGTTSTIQTAPTTSSSMQVSSGPSFPITNYLAPVSASISPS
SQ AVTSANGTVLKTTGASAVTSGGLMQIPTGFTLMSGGTMAQQVPVQAIQVHQAPQQTSPSS
SQ DSSTDLTQTSPSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLADGGLAVLNA
SQ FSQTPSAMQVSHSQVQDQGGVPQVFLTAPSGTVQIPVSAVQLHQMAVIGQQSSSGSSLTE
SQ LQXVNLDTSHNAKSD
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SC translated from EMBL #U50596
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FT 8 67 
SM00432; MADS.
FT 8 68 
PS50066; MADS_BOX_2.
FT 14 321 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 16 66 
PF00319; SRF-type transcription factor (DNA-binding a.
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SF phosphorylation;
SF both DNA-binding and C-terminal transactivation domains of SRF are necessary for synergistic transcriptional regulation by SRF and GATA-4 [6];
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CP stage 4 embryo: epiblast, blood islands [4]; stage 6 embryos: neural groove, neural plate, primitive streak, lateral plate mesoderm, underlying endoderm [4]; stage 8 embryo: neural folds, somites [4]; stage 11 embryo: neuroectoderm of brain and neural tube, developing heart, splanchnic mesoderm, somites, presomitic mesoderm [4]; embryo: day 18: brain, heart, gizzard, skeletal muscle [4]; additional in day 10 in aorta and in day 14 in 4th aortic arch and abdominal aorta [5]; upregulated in multinucleated (BudR for 60 hrs) and differentiated myotubes (84 hrs BudR) [4] [4] [5].
CN embryo: day 18 (mRNA and protein): liver [4]; in BudR replicating myoblasts [4] [4].
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FF binds to serum response element (SRE);
FF increases with myoblast differentiation;
FF required for alpha-actin transcription [4];
FF activates heart myocyte-specific transcription synergistically with GATA-4 [6];
FF in contrast to transcripts SRF protein appears predominantly during cardiac morphogenesis in developing myocardium and myotome [4];
FF SRF protein was abundant in the fusing paired primordia (HH stage 8) and myocardium (HH stage 10 to HH stage 15) of developing heart [4];
FF two mRNA species that correspond to 3.5 and 2.5 kb SRF mRNA were detected from RNA extracted from day 10 and 14 embryonic tissues, due to differential 3' polyadenylation [5];
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IN T00043 Arg80p; yeast, Saccharomyces cerevisiae.
IN T00250 Elk-1; human, Homo sapiens.
IN T01413 Net; mouse, Mus musculus.
IN T00737 SAP-1a; human, Homo sapiens.
IN T02128 SAP-1b; human, Homo sapiens.
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MX M00152 V$SRF_01.
MX M01257 V$SRF_02.
MX M00215 V$SRF_C.
MX M07618 V$SRF_Q3.
MX M00810 V$SRF_Q4.
MX M00922 V$SRF_Q5_01.
MX M01007 V$SRF_Q5_02.
MX M00186 V$SRF_Q6.
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BS R00031.
BS R14881.
BS R14969.
BS R14871.
BS R15858.
BS R04185.
BS R02246.
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DR TRANSPATH: MO000025157.
DR TRANSCOMPEL: C00428.
DR EMBL: U50596;
DR UniProtKB: Q90718;
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RN [1]; RE0001614.
RX PUBMED: 2005890.
RA Santoro I. M., Yi T.-M., Walsh K.
RT Identification of single-stranded-DNA-binding proteins that interact with muscle gene elements
RL Mol. Cell. Biol. 11:1944-1953 (1991).
RN [2]; RE0002948.
RX PUBMED: 1409704.
RA Lee T.-C., Shi Y., Schwartz R. J.
RT Displacement of BrdUrd-induced YY1 by serum response factor activates skeletal alpha-actin transcription in embryonic myoblasts
RL Proc. Natl. Acad. Sci. USA 89:9814-9818 (1992).
RN [3]; RE0002955.
RX PUBMED: 1328862.
RA Boulden A. M., Sealy L. J.
RT Maximal serum stimulation of the c-fos serum response element requires both the serum response factor and a novel binding factor, SRE-binding protein
RL Mol. Cell. Biol. 12:4769-4783 (1992).
RN [4]; RE0014921.
RX PUBMED: 8660892.
RA Croissant J.D., Kim J.H., Eichele G., Goering L., Lough J., Prywes R., Schwartz R.J.
RT Avian serum response factor expression restricted primarily to muscle cell lineages is required for alpha-actin gene transcription
RL Dev. Biol. 177:250-264 (1996).
RN [5]; RE0015323.
RX PUBMED: 8900044.
RA Chen C. Y., Croissant J., Majesky M., Topouzis S., McQuinn T., Frankovsky M. J., Schwartz R. J.
RT Activation of the cardiac alpha-actin promoter depends upon serum response factor, Tinman homologue, Nkx-2.5, and intact serum response elements
RL Dev. Genet. 19:119-130 (1996).
RN [6]; RE0015397.
RX PUBMED: 11038368.
RA Moore M. L., Wang G. L., Belaguli N. S., Schwartz R. J., McMillin J. B.
RT GATA-4 and Serum Response Factor Regulate Transcription of the Muscle-Specific Carnitine Palmitoyltransferase I {beta} in Rat Heart
RL J. Biol. Chem. 276:1026-1033 (2001).
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