AC T00737
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ID T00737
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DT 27.10.1992 (created); ewi.
DT 12.10.2011 (updated); lat.
CO Copyright (C), QIAGEN.
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FA SAP-1a
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SY Elk-4; SAP-1; SAP-1a; SRF accessory protein 1; SRF accessory protein 1a; SRF Associated Protein 1a.
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OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
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GE G007337 ELK4; HGNC: ELK4.
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HO TCF.
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CL C0016; ETS; 3.5.2.2.3.1.
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SZ 431 AA; 46.8 kDa (cDNA) (calc.), 58 kDa (SDS)
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SQ MDSAITLWQFLLQLLQKPQNKHMICWTSNDGQFKLLQAEEVARLWGIRKNKPNMNYDKLS
SQ RALRYYYVKNIIKKVNGQKFVYKFVSYPEILNMDPMTVGRIEGDCESLNFSEVSSSSKDV
SQ ENGGKDKPPQPGAKTSSRNDYIHSGLYSSFTLNSLNSSNVKLFKLIKTENPAEKLAEKKS
SQ PQEPTPSVIKFVTTPSKKPPVEPVAATISIGPSISPSSEETIQALETLVSPKLPSLEAPT
SQ SASNVMTAFATTPPISSIPPLQEPPRTPSPPLSSHPDIDTDIDSVASQPMELPENLSLEP
SQ KDQDSVLLEKDKVNNSSRSKKPKGLGLAPTLVITSSDPSPLGILSPSLPTASLTPAFFSQ
SQ TPIILTPSPLLSSIHFWSTLSPVAPLSPARLQGANTLFQFPSVLNSHGPFTLSGLDGPST
SQ PGPFSPDLQKT
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SC Swiss-Prot#P28324-1
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FT 4 87 
PF00178; Ets-domain.
FT 4 89 
SM00413; etsneu2.
FT 5 85 
PS50061; ETS_DOMAIN_3.
FT 7 75 
PS50140; HSF_ETS.
FT 381 381 
Serin-phosphorylation by MAP kinase(-like) activity [7].
XX
SF revised sequence [5];
SF a splice variant is SAP-1b;
SF three regions demonstrate similarity with Elk-1, one of them (box C) largely truncated in SAP-1b [5];
SF ternary complex formation with SRE and SRF is independent of orientation and distance to the SRF site [4] [5];
SF box C may reduce ternary complex formation [5];
SF the crystal structure of the Sap-1 ETS domain bound to DNA is resolved [10];
SF X-ray structure of the Sap1-SRF-SRE ternary complex is determined [11];
SF phosphorylation-dependent activation of SAP-1a involves Ser-381, Ser-387, Thr-420 and Ser-425 [7];
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CP K562, HeLa, Jurkat, IARC-EW17, Saos, BL2, Ramos, E418 [8].
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FF activator;
FF contacts a GGA core DNA sequence [10];
FF cooperates with SRF T00764 in activating transcription from c-fos SRE [5] [6];
FF phosphorylation by ERK2 results in increased SRF-dependent and SRF-independent DNA binding [6] [7];
FF mediates insulin-increased transcription of the prolactin gene in pituitary cells [9];
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IN T21852 HDAC1; Mammalia.
IN T00765 SRF-L; mouse, Mus musculus.
IN T00761 SRF; chick, Gallus gallus.
IN T00762 SRF; cat, Felis silvestris catus.
IN T00763 SRF; clawed frog, Xenopus laevis.
IN T00764 SRF; human, Homo sapiens.
IN T00766 SRF; rat, Rattus norvegicus.
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MX M00771 V$ETS_Q4.
MX M00971 V$ETS_Q6.
MX M01167 V$SAP1A_01.
MX M03844 V$SAP1A_Q6.
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BS R03598.
BS R03600.
BS R03603.
BS R03604.
BS R23439.
BS R23442.
BS R23443.
BS R23444.
BS R23445.
BS R23446.
BS R23447.
BS R23448.
BS R23449.
BS R23450.
BS R23451.
BS R23452.
BS R23453.
BS R23454.
BS R23455.
BS R23456.
BS R23457.
BS R23458.
BS R23459.
BS R23460.
BS R02386.
BS R00040.
BS R00460.
BS R00466.
BS R31525.
BS R36824.
BS R00961.
BS R03582.
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DR TRANSPATH: MO000019546.
DR TRANSCOMPEL: C00022.
DR EMBL: M85165; HSSERREFB.
DR UniProtKB: P28324-1;
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RN [1]; RE0000068.
RX PUBMED: 2492906.
RA Shaw P. E., Schroeter H., Nordheim A.
RT The Ability of a Ternary Complex to Form Over the Serum Response Element Correlates with Serum Inducibility of the Human c-fos Promoter
RL Cell 56:563-572 (1989).
RN [2]; RE0000195.
RX PUBMED: 1339307.
RA Dalton S., Treisman R.
RT Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element
RL Cell 68:597-612 (1992).
RN [3]; RE0000343.
RX PUBMED: 2108863.
RA Schroeter H., Mueller C. G. F., Meese K., Nordheim A.
RT Synergism in ternary complex formation between the dimeric glycoprotein p67SRF, polypeptide p62TCF and the c-fos serum response element
RL EMBO J. 9:1123-1130 (1990).
RN [4]; RE0000531.
RX PUBMED: 1425594.
RA Treisman R., Marais R., Wynne J.
RT Spatial flexibility in ternary complexes between SRF and its accessory proteins
RL EMBO J. 11:4631-4640 (1992).
RN [5]; RE0004454.
RX PUBMED: 8293474.
RA Dalton S., Treisman R.
RT Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element
RL Cell 76:411-411 (1994).
RN [6]; RE0004468.
RX PUBMED: 8668149.
RA Shore P., Whitmarsh A. J., Bhaskaran R., Davis R. J., Waltho J. P., Sharrocks A. D.
RT Determinants of DNA-binding specificity of ets-domain transcription factors
RL Mol. Cell. Biol. 16:3338-3349 (1996).
RN [7]; RE0005332.
RX PUBMED: 7540136.
RA Price M. A., Rogers A. E., Treisman R.
RT Comparative analysis of the ternary complex factors Elk-1, SAP-1a and SAP-2 (ERP/NET)
RL EMBO J. 14:2589-2601 (1995).
RN [8]; RE0015517.
RX PUBMED: 8764983.
RA Magnaghi-Jaulin L., Masutani H., Lipinski M., Harel-Bellan A.
RT Analysis of SRF, SAP-1 and ELK-1 transcripts and proteins in human cell lines
RL FEBS Lett. 391:247-251 (1996).
RN [9]; RE0023726.
RX PUBMED: 11340077.
RA Jacob K. K., Stanley F. M.
RT Elk-1, C/EBPalpha, and Pit-1 confer an insulin-responsive phenotype on prolactin promoter expression in Chinese hamster ovary cells and define the factors required for insulin-increased transcription.
RL J. Biol. Chem. 276:24931-24936 (2001).
RN [10]; RE0024586.
RX PUBMED: 9734357.
RA Mo Y., Vaessen B., Johnston K., Marmorstein R.
RT Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
RL Mol. Cell 2:201-212 (1998).
RN [11]; RE0024594.
RX PUBMED: 11406578.
RA Hassler M., Richmond T. J.
RT The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex.
RL EMBO J. 20:3018-3028 (2001).
RN [12]; RE0048074.
RX PUBMED: 11283259.
RA Yang S. H., Vickers E., Brehm A., Kouzarides T., Sharrocks A. D.
RT Temporal recruitment of the mSin3A-histone deacetylase corepressor complex to the ETS domain transcription factor Elk-1.
RL Mol. Cell. Biol. 21:2802-2814 (2001).
RN [13]; RE0066975.
RX PUBMED: 20145255.
RA Fernandez-Alvarez A., Soledad Alvarez M., Cucarella C., Casado M.
RT Characterization of the human insulin-induced gene 2 (INSIG2) promoter: the role of Ets-binding motifs.
RL J. Biol. Chem. 285:11765-11774 (2010).
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