AC T00817
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ID T00817
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DT 15.10.1992 (created); ewi.
DT 14.12.2012 (updated); mkl.
CO Copyright (C), QIAGEN.
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FA TFIIA
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SY DBF3; TFIIA; TFIIAalpha/beta; TFIIAalpha/beta precursor.
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OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
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SF three subunits: alpha, beta, gamma [5];
SF alpha and beta are processed from a common alpha/beta precursor T02216;
SF holo-TFIIA is active either as dimeric alpha/beta + gamma or as trimeric alpha+beta+gamma complex [6] [7];
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CP (HeLa).
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FF stimulates basal transcription, but is not indispensable for pol II transcription [2] [5];
FF binds to and stabilizes TFIID/TBP-TATA box-complexes [2];
FF it decreases the influence of sequences flanking the TATA-box, casus a conformational change of TBP and neutralizes the inhibitory effect of the TBP N-terminus [4];
FF overcomes the repressing effect of DNA topoisomerase I DR2 and of certain TFIID components, thus acting as an antirepressor in addition to actively stimulating transcription [7] [8] [12];
FF interaction with TBP is competed by negative cofactor Dr1 / NC2 T00197 [1];
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IN T00671 p53; human, Homo sapiens.
IN T02137 PC4; human, Homo sapiens.
IN T00794 TBP; human, Homo sapiens.
IN T00820 TFIID; human, Homo sapiens.
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MX M00707 V$TFIIA_Q6.
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BS R00996.
BS R01002.
BS R01015.
BS R01016.
BS R01017.
BS R01018.
BS R01605.
BS R03170.
BS R00705.
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DR TRANSPATH: MO000025192.
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RN [1]; RE0000229.
RX PUBMED: 1934060.
RA Meisterernst M., Roeder R. G.
RT Family of proteins that interact with TFIID and regulate promoter activity
RL Cell 67:557-567 (1991).
RN [2]; RE0000875.
RX PUBMED: 3818643.
RA Reinberg D., Horikoshi M., Roeder R. G.
RT Factors involved in specific transcription by mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site
RL J. Biol. Chem. 262:3322-3330 (1987).
RN [3]; RE0002734.
RX PUBMED: 2334420.
RA Yu X.-B., Burke C., Zhang J., Marchitelli L. J., Davis E. A., Ackermann S.
RT Transcription factor IIA of wheat and human interacts similarly with the adenovirus-2 major late promoter
RL Biochem. Biophys. Res. Commun. 168:498-505 (1990).
RN [4]; RE0005578.
RX PUBMED: 1406690.
RA Lee D. K., Dejong J., Hashimoto S., Horikoshi M., Roeder R. G.
RT TFIIA induces conformational changes in TFIID via interactions with the basic repeat
RL Mol. Cell. Biol. 12:5189-5196 (1992).
RN [5]; RE0005655.
RX PUBMED: 1729613.
RA Cortes P., Flores O., Reinberg D.
RT Factors involved in specific transcription by mammalian RNA polymerase II: Purification and analysis of transcription factor IIA and identification of transcription factor IIJ
RL Mol. Cell. Biol. 12:413-421 (1992).
RN [6]; RE0005656.
RX PUBMED: 7958899.
RA Ozer J., Moore P. A., Bolden A. H., Lee A., Rosen C. A., Lieberman P. M.
RT Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription
RL Genes Dev. 8:2324-2335 (1994).
RN [7]; RE0005657.
RX PUBMED: 7958900.
RA Sun X., Ma D., Sheldon M., Yeung K., Reinberg D.
RT Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription
RL Genes Dev. 8:2336-2348 (1994).
RN [8]; RE0005658.
RX PUBMED: 8224850.
RA Ma D., Watanabe H., Mermelstein F., Admon A., Oguri K., Sun X., Wada T., Imai T., Shiroya T., Reinberg D., Handa H.
RT Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription
RL Genes Dev. 7:2246-2257 (1993).
RN [9]; RE0005659.
RX PUBMED: 8224848.
RA Dejong J., Roeder R. G.
RT A single cDNA hTFIIA/alpha encodes both the p35 and p19 subunits of human TFIIA
RL Genes Dev. 7:2220-2234 (1993).
RN [10]; RE0005660.
RX PUBMED: 2065666.
RA Usuda Y., Kubota A., Berk A. J., Handa H.
RT Affinity purification of transcription factor IIA from HeLa cell nuclear extracts
RL EMBO J. 10:2305-2310 (1991).
RN [11]; RE0005661.
RX PUBMED: 1465382.
RA Bungert J., Waldschmidt R., Kober I., Seifart K. H.
RT Transcription factor IIA is inactivated during terminal differentiation of avian erythroid cells
RL Proc. Natl. Acad. Sci. USA 89:11678-11682 (1992).
RN [12]; RE0005662.
RX PUBMED: 8396729.
RA Merino A., Madden K. R., Lane W. S., Champoux J. J., Reinberg D.
RT DNA topoisomerase I is involved in both repression and activation of transcription
RL Nature 365:227-232 (1993).
RN [13]; RE0005663.
RX PUBMED: 7724559.
RA Dejong J., Bernstein R., Roeder R. G.
RT Human general transcription factor TFIIA: Characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription
RL Proc. Natl. Acad. Sci. USA 92:3313-3317 (1995).
RN [14]; RE0005664.
RX PUBMED: 8692860.
RA Ma D., Olave I., Merino A., Reinberg D.
RT Separation of the transcriptional coactivator and antirepression functions of transcription factor IIA
RL Proc. Natl. Acad. Sci. USA 93:6583-6588 (1996).
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