AC T02119
XX
ID T02119
XX
DT 21.05.1997 (created); hiwi.
DT 07.08.2013 (updated); mkl.
CO Copyright (C), QIAGEN.
XX
FA TAFII250
XX
SY p230; p250; TAF(II)250; TAF-230.
XX
OS fruit fly, Drosophila melanogaster
OC eukaryota; animalia; metazoa; arthropoda; insecta; diptera; drosophiloidea; drosophilidae
XX
SZ 2068 AA; 232.5 kDa (cDNA) (calc.), 250 kDa (SDS), 230 kDa (SDS)
XX
SQ MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLS
SQ KLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASGGMSAFDALKAGVKREEREDGAV
SQ KAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLTKDDKELMPPPSAP
SQ MRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPD
SQ FRPQKVLRFSRLFGPGKPTSLPQIWRHVRQSRRKRNQSRDQKTTNTGGSDSPSDTEEPRK
SQ RGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQI
SQ WYDILEVPDSGEGFNYGFKTKAASTSSQQQLKDERRVKSPEDDVEDPSIADDAFLMVSQL
SQ HWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQG
SQ SGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVL
SQ TLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTP
SQ PPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMNV
SQ RAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLS
SQ GRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDYVYGEVAFAHTSPFLG
SQ ILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVP
SQ GPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADF
SQ KRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQ
SQ EDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKP
SQ TQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLS
SQ TEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSAS
SQ EESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGK
SQ DDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQ
SQ FATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSG
SQ GHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSN
SQ PSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVP
SQ RDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLF
SQ PVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYT
SQ LAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPF
SQ LKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTR
SQ YTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSR
SQ ASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPR
SQ KQRDPVEEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKN
SQ IKTEIDLEAHQMADESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNG
SQ PYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRV
SQ RIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQ
SQ HQVMPPMQSEQLQQQQTPQGDNDYAWTF
XX
SC Swiss-Prot#P51123
XX
FT 1 342 
interaction with TAF-110 [11].
FT 146 986 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 1247 1360 
HMG box homology [4].
FT 1471 1579 
SM00297; bromo_6.
FT 1478 1565 
PF00439; Bromodomain.
FT 1490 1560 
PS50014; BROMODOMAIN_2.
FT 1593 1701 
SM00297; bromo_6.
FT 1600 1687 
PF00439; Bromodomain.
FT 1612 1682 
PS50014; BROMODOMAIN_2.
FT 1793 1805 
PF02178; AT_hook.
XX
SF interacts with TBP through two regions thereby inhibiting DNA-binding by TBP in the absence of other TAFs;
SF for this, the binding site within the N-terminal 80 amino acid residues is responsible [3] [10];
SF most other TAFs interact with TAFII250;
SF doubtful significance of the HMG homology region [4];
XX
FF tight association with TBP, contributes to TFIID assembly;
FF confers promoter selectivity to TBP-TAF complexes (TFIID subcomplexes) together with TAF(II)150 in mediating the requirement for initiator and additional downstream elements [8];
FF function may partially be substituted by SV40 large T antigen [9];
XX
IN T01269 Taf2p; yeast, Saccharomyces cerevisiae.
IN T02121 TAFII110; fruit fly, Drosophila melanogaster.
IN T02120 TAFII150; fruit fly, Drosophila melanogaster.
IN T02131 TAFII30beta; fruit fly, Drosophila melanogaster.
IN T02124 TAFII60; fruit fly, Drosophila melanogaster.
IN T00797 TBP; fruit fly, Drosophila melanogaster.
XX
DR TRANSPATH: MO000045983.
DR EMBL: S61883; S61883.
DR UniProtKB: P51123; CCG1_DROME.
DR FLYBASE: FBgn0010355; Taf250.
XX
RN [1]; RE0005557.
RX PUBMED: 1657708.
RA Pugh B. F., Tjian R.
RT Transcription from a TATA-less promoter requires a multisubunit TFIID complex
RL Genes Dev. 5:1935-1945 (1991).
RN [2]; RE0005560.
RX PUBMED: 8262073.
RA Weinzierl R. O. J., Ruppert S., Dynlacht B. D., Tanese N., Tjian R.
RT Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID
RL EMBO J. 12:5303-5309 (1993).
RN [3]; RE0005567.
RX PUBMED: 8170939.
RA Kokubo T., Yamashita S., Horikoshi M., Roeder R. G., Nakatani Y.
RT Interaction between the N-terminal domain of the 230-kDa subunit and the TATA box-binding subunit of TFIID negatively regulates TATA-box binding
RL Proc. Natl. Acad. Sci. USA 91:3520-3524 (1994).
RN [4]; RE0005568.
RX PUBMED: 8464492.
RA Weinzierl R. O. J., Dynlacht B. D., Tjian R.
RT Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator
RL Nature 362:511-517 (1993).
RN [5]; RE0005569.
RX PUBMED: 8349634.
RA Kokubo T., Takada R., Yamashita S., Gong D.-W., Roeder R. G., Horikoshi M., Nakatani Y.
RT Identification of TFIID components required for transcriptional activation by upstream stimulatory factor
RL J. Biol. Chem. 268:17554-17558 (1993).
RN [6]; RE0005570.
RX PUBMED: 7545910.
RA Kokubo T., Gong D.-W., Wootton J. C., Horikoshi M., Roeder R. G., Nakatani Y.
RT Molecular cloning of Drosophila TFIID subunits
RL Nature 367:484-487 (1994).
RN [7]; RE0005571.
RX PUBMED: 8178153.
RA Verrijzer C. P., Yokomori K., Chen J.-L., Tjian R.
RT Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA
RL Science 264:933-941 (1994).
RN [8]; RE0005596.
RX PUBMED: 7600579.
RA Verrijzer C. P., Chen J.-L., Yokomori K., Tjian R.
RT Binding of TAFs to Core Elements Directs Promotor Selectivity by RNA Polymerase II
RL Cell 81:1115-1125 (1995).
RN [9]; RE0005597.
RX PUBMED: 8647434.
RA Damania B., Alwine J. C.
RT TAF-like function of SV40 large T antigen
RL Genes Dev. 10:1369-1381 (1996).
RN [10]; RE0005598.
RX PUBMED: 8504928.
RA Kokubo T., Gong D.-W., Yamashita S., Horikoshi M., Roeder R. G., Nakatani Y.
RT Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID
RL Genes Dev. 7:1033-1046 (1993).
RN [11]; RE0005599.
RX PUBMED: 8327460.
RA Kokubo T., Gong D.-W., Roeder R. G., Horikoshi M., Nakatani Y.
RT The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit
RL Proc. Natl. Acad. Sci. USA 90:5896-5900 (1993).
RN [12]; RE0005600.
RX PUBMED: 8276241.
RA Yokomori K., Chen J.-L., Zhou S., Tjian R.
RT Molecular cloning and characterization of dTAFII30alpha and dTAFII308beta: two small subunits of Drosophila TFIID
RL Genes Dev. 7:2587-2597 (1993).
XX
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