AC T05970
XX
ID T05970
XX
DT 14.05.2004 (created); oke.
DT 02.04.2014 (updated); yre.
CO Copyright (C), QIAGEN.
XX
FA myocardin-isoform1
XX
SY BSAC2A; myocardin; MyoCD; Srfcp.
XX
OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
XX
GE G009497 Myocd.
XX
SZ 935 AA; 101.4 kDa (cDNA) (calc.).
XX
SQ MTLLGSEHSLLIRRKFRSVLQLRLQQRRTQEQLANQGLIPPLKGPTEFHDPRKQLDSAKT
SQ EDSLRRKGRNRSDRASLVTMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRQGPLE
SQ LVEKNILPMDSSVKEAIKGTEVSLSKAADAFAFEDDSSRDGLSPDQARSEDPQGSTGSTP
SQ DIKSTEAPLDTIQDLTPGSESDKNDAASQPGNQSDPGKQVLGPLSTPIPVHTAVKSKSLG
SQ DSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQ
SQ QQQQQQQQQQQQQQQQQQQQRFSYPGMHQTHLKEPNEQMARNPNPSSTPLSNTPLSPVKN
SQ SISGQTGVSSLKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALVDRLRPFQDCAGNP
SQ VPNFGDITTVTFPVTPNTLPSYQSSPTGFYHFGSTSSSPPISPASSDLSAAGSLPDTFTD
SQ ASPGFGLHASPVPACTDESLLSSLNGGSGPSEPDGLDSEKDKMLVEKQKVINQLTWKLRQ
SQ EQRQVEELRMQLQKQKSSCSDQKPLPFLATTIKQEDVSSCPFAPQQASGKGQGHSSDSPP
SQ PACETAQLLPHCVESSGQTHVLSSTFLSPQCSPQHSPLGGLKSPQHISLPPSPNNHYFLA
SQ SSSGAQRENHGVSSPSSSQGCAQMTGLQSSDKVGPTFSIPSPTFSKSSSAVSDITQPPSY
SQ EDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKIPKIPGSSCSPTAIPPKPSA
SQ SFEQASSGGQMAFGHYANDSDEHLEVLLNSHSPIGKVSDVTLLKIGSEEPPFDSIMDGFP
SQ GKAAEDLFSAHELLPGPLSPMHAQLSPPSVDSSGLQLSFTESPWETMEWLDLTPPSSTPG
SQ FSNLTSSGPSIFNIDFLDVTDLNLNSPMDLHLQQW
XX
SC translated from EMBL #AF384055
XX
FT 18 43 
PS51073; RPEL.
FT 18 43 
SM00707; rpel.
FT 60 915 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 62 87 
PS51073; RPEL.
FT 62 87 
SM00707; rpel.
FT 106 131 
PS51073; RPEL.
FT 106 131 
SM00707; rpel.
FT 243 260 
basic domain [2].
FT 287 320 
glutamine-rich region (27/34) [2].
FT 380 414 
SAP domain, interaction with SRF [2].
FT 380 414 PF02037; SAP domain.
FT 380 414 PS50800; SAP.
FT 380 414 SM00513; sap_9.
FT 524 552 Leucine Zipper-like [2].
XX
SF belongs to the SAP (SAF-A/B, Acinus, PIAS) domain family of nuclear proteins [2];
SF two splice variants are known;
XX
CP embryonic cardiac and smooth muscle cells; myocardium after birth [2]; in adult mice: aorta, bladder, stomach, intestine, colon [3] [2] [3].
XX
FF highly potent activator [2];
FF forms ternary complexes with SRF on CArG boxes, interacts with SRF rather than binds DNA [2] [3];
FF provides a mechanism by which SRF can regulate muscle-specific genes [2] [3];
FF interacts with SRF T00766 and can be displaced by Elk-1 T05966 [1];
FF myocardin siRNA markedly decreased activity of smooth muscle-specific promoters [3];
FF transactivation of the ANF promoter by myocardin [4];
XX
IN T11166 FKHL5; mouse, Mus musculus.
IN T24705 HFH-1; Mammalia.
IN T00764 SRF; human, Homo sapiens.
IN T16471 SRF; Mammalia.
IN T17039 TEF-1; Mammalia.
XX
BS R33433.
XX
DR TRANSPATH: MO000041989.
DR EMBL: AF384055;
DR UniProtKB: Q8VIM5;
XX
RN [1]; RE0024249.
RX PUBMED: 15014501.
RA Wang Z., Wang D. Z., Hockemeyer D., McAnally J., Nordheim A., Olson E. N.
RT Myocardin and ternary complex factors compete for SRF to control smooth muscle gene expression.
RL Nature 428:185-189 (2004).
RN [2]; RE0024258.
RX PUBMED: 11439182.
RA Wang D., Chang P. S., Wang Z., Sutherland L., Richardson J. A., Small E., Krieg P. A., Olson E. N.
RT Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor.
RL Cell 105:851-862 (2001).
RN [3]; RE0024260.
RX PUBMED: 12663482.
RA Yoshida T., Sinha S., Dandre F., Wamhoff B. R., Hoofnagle M. H., Kremer B. E., Wang D. Z., Olson E. N., Owens G. K.
RT Myocardin is a key regulator of CArG-dependent transcription of multiple smooth muscle marker genes.
RL Circ. Res. 92:856-864 (2003).
RN [4]; RE0025135.
RX PUBMED: 14645532.
RA Ueyama T., Kasahara H., Ishiwata T., Nie Q., Izumo S.
RT Myocardin expression is regulated by Nkx2.5, and its function is required for cardiomyogenesis.
RL Mol. Cell. Biol. 23:9222-9232 (2003).
XX
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