AC T00068
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ID T00068
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DT 14.04.1993 (created); ewi.
DT 29.11.2012 (updated); mkl.
CO Copyright (C), QIAGEN.
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FA Brf1p
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SY BRF; BRF1; BRF1p; TAF-70; TDS4; TFIIIB-p70; YGR246C.
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OS yeast, Saccharomyces cerevisiae
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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GE G004161 BRF1.
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HO TFIIIB90 (human) [5].
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SZ 596 AA; 66.9 kDa (calc.), 70 kDa (SDS)
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SQ MPVCKNCHGTEFERDLSNANNDLVCKACGVVSEDNPIVSEVTFGETSAGAAVVQGSFIGA
SQ GQSHAAFGGSSALESREATLNNARRKLRAVSYALHIPEYITDAAFQWYKLALANNFVQGR
SQ RSQNVIASCLYVACRKEKTHHMLIDFSSRLQVSVYSIGATFLKMVKKLHITELPLADPSL
SQ FIQHFAEKLDLADKKIKVVKDAVKLAQRMSKDWMFEGRRPAGIAGACILLACRMNNLRRT
SQ HTEIVAVSHVAEETLQQRLNEFKNTKAAKLSVQKFRENDVEDGEARPPSFVKNRKKERKI
SQ KDSLDKEEMFQTSEEALNKNPILTQVLGEQELSSKEVLFYLKQFSERRARVVERIKATNG
SQ IDGENIYHEGSENETRKRKLSEVSIQNEHVEGEDKETEGTEEKVKKVKTKTSEEKKENES
SQ GHFQDAIDGYSLETDPYCPRNLHLLPTTDTYLSKVSDDPDNLEDVDDEELNAHLLNEEAS
SQ KLKERIWIGLNADFLLEQESKRLKQEADIATGNTSVKKKRTRRRNNTRSDEPTKTVDAAA
SQ AIGLMSDLQDKSGLHAALKAAEESGDFTTADSVKNMLQKASFSKKINYDAIDGLFR
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SC Swiss-Prot#P29056
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FT 85 166 
SM00385; cyclin_7.
FT 87 157 
PF00382; Transcription factor TFIIB repeat.
FT 180 264 
SM00385; cyclin_7.
FT 182 255 
PF00382; Transcription factor TFIIB repeat.
FT 461 595 
PF07741; Brf1-like TBP-binding domain.
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SF N-terminus homologous to TFIIB;
SF part of the TFIIIB complex that comprises TBP T00798, Brf1p, and TFC5 T00055 [4];
SF C-terminus contains a masked DNA-binding domain which binds DNA very tightly without sequence specificity [4];
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FF pol III transcription factor;
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IN T00798 Spt15p; yeast, Saccharomyces cerevisiae.
IN T00794 TBP; human, Homo sapiens.
IN T00795 TBP; fission yeast, Schizosaccharomyces pombe.
IN T00796 TBP; mouse, Mus musculus.
IN T00797 TBP; fruit fly, Drosophila melanogaster.
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BS R23019.
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DR EMBL: L00630; SCTFIIIB.
DR EMBL: M91073; SCTFIIBRF.
DR UniProtKB: P29056; TF3B_YEAST.
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RN [1]; RE0000223.
RX PUBMED: 1458536.
RA Kassavetis G. A., Joazeiro C. A. P., Pisano M., Geiduschek E. P., Colbert T., Hahn S., Blanco J. A.
RT The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB
RL Cell 71:1055-1064 (1992).
RN [2]; RE0000745.
RX PUBMED: 1398071.
RA Colbert T., Hahn S.
RT A yeast TFIIB-related factor involved in RNA polymerase III transcription
RL Genes Dev. 6:1940-1949 (1992).
RN [3]; RE0001059.
RX PUBMED: 8340360.
RA Poon D., Weil P. A.
RT Immunopurification of yeast TATA-binding protein and associated factors
RL J. Biol. Chem. 268:15325-15328 (1993).
RN [4]; RE0015990.
RX PUBMED: 9218475.
RA Huet J., Conesa C., Carles C., Sentenac A.
RT A cryptic DNA binding domain at the COOH terminus of TFIIIB70 affects formation, stability, and function of preinitiation complexes.
RL J. Biol. Chem. 272:18341-18349 (1997).
RN [5]; RE0015991.
RX PUBMED: 11121026.
RA Teichmann M., Wang Z., Roeder R. G.
RT A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements.
RL Proc. Natl. Acad. Sci. USA 97:14200-14205 (2000).
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//