TRANSFAC FACTOR TABLE, Release 2017.2 - public - 2017-06-30, (C) QIAGEN

AC T00930 XX ID T00930 XX DT 15.06.1993 (created); ewi. DT 23.09.2013 (updated); spk. CO Copyright (C), QIAGEN. XX FA LEF-1 XX SY LEF 1; LEF-1L; lymphocyte enhancer binding factor 1; lymphoid enhancer binding factor 1. XX OS mouse, Mus musculus OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae XX GE G013561 Lef1. XX CL C0015; HMG; XX SZ 397 AA; 44.1 kDa (cDNA) (calc.), 54KDa (SDS) XX SQ MPQLSGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESE SQ IIPASNGHEVVRQAPSSQEPYHDKAREHPDEGKHPDGGLYNKGPSYSSYSGYIMMPNMNS SQ DPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQ SQ GMSRHPPAPEIPTFYPLSPGGVGQITPPIGWQGQPVYPITGGFRQPYPSSLSGDTSMSRF SQ SHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKP SQ LNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQL SQ YPGWSARDNYGKKKKRKREKLQESTSGTGPRMTAAYI XX SC Swiss-Prot#P27782-1 XX FT 12 50 acidic region (14/36) [1]. FT 75 271 proline-rich region (37/197) [1]. FT 99 217 context-dependent activation domain CAD [9]. FT 296 366 SM00398; hmgende2. FT 297 365 PF00505; HMG (high mobility group) box. FT 297 365 PS50118; HMG_BOX_2. FT 372 380 B box (essential for nuclear transposition) [15]. FT 387 397 LEF-1N tail [22]. XX SF DNA binding affinity of the monomer: KD = 1 nM [3]; SF contacts DNA from one side [3]; SF induces a bend of about 117-130 degree [4] [7]; SF widens and binds the minor groove of DNA [4] [7]; SF the L-shape HMG domain also binds across the narrowed major groove [7]; SF expression can be activated by BMP-4, which suggests a regulatory role of LEF-1 in BMP-mediated inductive tissue interaction [13]; SF LEF-1 translocates beta-Catenin to the nucleus [19] [9]; SF truncated LEF-1 (missing the beta-Catenin binding domain) is present in the nucleus but cannot translocate beta-Catenin to the nucleus [9]; SF gene located on chromosome 3 [3]; SF interaction with beta-Catenin and Plakoglobin [19]; SF N terminally deleted LEF-1 is no longer able to interact with beta-Catenin but still binds to specific DNA fragments [19]; SF alanine substitution mutagenesis and EMSA shows that AA 25-27 and 32-34 are not necessary for beta-Catenin binding [21]; SF for the interaction of beta-catenin and LEF-1 the HMG domain of LEF-1 is not essential [9]; SF circular permutation analysis of DNA bending induced by LEF-1 or the beta-catenin/ LEF-1 complex showed that beta-catenin decreased the extent of LEF-1 induced DNA bending (from about 120� to about 80�) [9]; XX CP pre-B cells, T lymphocytes [1]; during embryogenesis: neural crest, mesencephalon, tooth germs, whisker follicles [11]; (embr.<10.5) limb buds, neural crest, pharyngeal arches, nasal process; (embr. < 15.5): lung, urogenital system, tooth buds, thymus and choroid plexus, tail prevertebrae, brain and inner ear [10] [10] [11] [1]. XX FF potent activator when bound in the right context, e. g. with an adjacently binding TCF-2 [5]; FF activator, possibly by augmenting an optimal chromatin architecture which facilitates other factors to assemble to a transcription complex [4] [24]; FF for expression in teeth, see [23]; FF for regulation of inductive tissue interactions in tooth and hair development see [13]; FF LEF-1 HMG domain binds to irregular DNA structures although with a roughly 2-fold lower affinity than a specific duplex binding site and induces negative supercoils (about half a helical turn per HMG box); FF regulation of the TCF alpha enhancer is dependent on the wild-type LEF-1 binding site which induces a bending angle of 130� into the DNA; FF a mutated LEF-1 binding site leads to a lower bending angle and to lower activity of the TCF alpha enhancer [14]; FF upon Wnt-1 signaling combined with beta-Catenin binding to LEF-1 the transcriptional activity rises; FF taken together LEF-1 can exert two different roles: in association with beta-Catenin it can mediate transcriptional activation in a context-independent manner, whereas it regulates - together with ALY - transcription in a context specific way [21]; FF interacts with CBP [20]; XX IN T02977 armadillo; fruit fly, Drosophila melanogaster. IN T02872 beta-catenin; human, Homo sapiens. IN T02984 beta-catenin; mouse, Mus musculus. IN T03236 CBP; fruit fly, Drosophila melanogaster. IN T25615 NICD1; human, Homo sapiens. IN T04096 Smad3-isoform1; human, Homo sapiens. IN T04292 Smad4; human, Homo sapiens. XX MX M00978 V$LEF1TCF1_Q4. MX M02878 V$LEF1_03. MX M02774 V$LEF1_04. MX M00805 V$LEF1_Q2. MX M01022 V$LEF1_Q2_01. MX M07046 V$LEF1_Q3. MX M07605 V$LEF1_Q4. MX M02019 V$LEF1_Q5. MX M03794 V$LEF1_Q5_01. MX M08902 V$TCF7RELATED_Q4. XX BS R08636. BS R56947. BS R56949. BS R56950. BS R56951. BS R03576. BS R30769. BS R04450. BS R08631. BS R08823. BS R08824. BS R56953. BS R56954. BS R56956. BS R56957. BS R03470. BS R08809. BS R38307. BS R38308. BS R16083. BS R08816. BS R02248. BS R08649. BS R08633. BS R08634. BS R13042. XX DR TRANSPATH: MO000018859. DR TRANSCOMPEL: C00194. DR EMBL: X58636; MMLEF1. DR UniProtKB: P27782-1; XX RN [1]; RE0000695. RX PUBMED: 1827423. RA Travis A., Amsterdam A., Belanger C., Grosschedl R. RT LEF-1, a gene encoding a lymphoid-specific with protein, an HMG domain, regulates T-cell receptor alpha enhancer function RL Genes Dev. 5:880-894 (1991). RN [2]; RE0002106. RX PUBMED: 1861981. RA Aronheim A., Ohlsson H., Park C. W., Edlund T., Walker M. D. RT Distribution and characterization of helix-loop-helix enhancer-binding proteins from pancreatic beta cells and lymphocytes RL Nucleic Acids Res. 19:3893-3899 (1991). RN [3]; RE0004363. RX PUBMED: 1752444. RA Giese K., Amsterdam A., Grosschedl R. RT DNA-binding properties of the HMG domain of the lymphoid-specific transcriptional regulator LEF-1 RL Genes Dev. 5:2567-2578 (1991). RN [4]; RE0004364. RX PUBMED: 1555239. RA Giese K., Cox J., Grosschedl R. RT The HMG domain of lymphoid enhancer factor 1 bends DNA and facilitates assembly of functional nucleoprotein structures RL Cell 69:185-195 (1992). RN [5]; RE0004365. RX PUBMED: 8253387. RA Carlsson P., Waterman M. L., Jones K. A. RT The hLEF/TCF-1alpha HMG protein contains a context-dependent transcriptional activation domain that induces the TCRalpha enhancer in T cells RL Genes Dev. 7:2418-2430 (1993). RN [6]; RE0004367. RX PUBMED: 8346022. RA Read C. M., Cary P. D., Crane-Robinson C., Driscoll P. C., Norman D. G. RT Solution structure of a DNA-binding domain from HMG1 RL Nucleic Acids Res. 21:3427-3436 (1993). RN [7]; RE0004369. RX PUBMED: 7651541. RA Love J. J., Li X., Case D. A., Giese K., Grosschedl R., Wright P. E. RT Structural basis for DNA bending by the architectural transcription factor LEF-1 RL Nature 376:791-795 (1995). RN [8]; RE0004382. RX PUBMED: 8467791. RA Weir H. M., Kraulis P. J., Hill C. S., Raine A. R. C., Laue E. D., Thomas J. O. RT Structure of the HMG box motif in the B-domain of HMG1 RL EMBO J. 12:1311-1319 (1993). RN [9]; RE0006108. RX PUBMED: 8757136. RA Behrens J., von Kries J. P., Kuehl M., Bruhn L., Wedlich D., Grosschedl R., Birchmeier W. RT Functional interaction of beta-catenin with the transcription factor LEF -1 RL Nature 382:638-642 (1996). RN [10]; RE0006815. RX PUBMED: 8223271. RA Oosterwegel M., van de Wetering M., Timmerman J., Kruisbeek A., Destree O., Meijlink F., Clevers H. RT Differential expression of the HMG box factors TCF-1 and LEF-1 during murine embryogenesis RL Development 118:439-448 (1993). RN [11]; RE0009773. RX PUBMED: 7958926. RA van Genderen C., Okamura R. M., Farinas I., Quo R.-G., Parslow T. G., Bruhn L., Grosschedl R. RT Development of several organs that require inductive epithelial-mesenchymal interactions is impaired in LEF-1-deficient mice RL Genes Dev. 8:2691- 2703 (1994). RN [12]; RE0010385. RX PUBMED: 7537238. RA Zhou P., Byrne C., Jacobs J., Fuchs E. RT Lymphoid enhancer factor 1 directs hair follicle patterning and epithelial cell fate RL Genes Dev. 9:700-713 (1995). RN [13]; RE0012617. RX PUBMED: 8647435. RA Kratochwil K., Dull M., Farinas I., Galceran J., Grosschedl R. RT Lef1 expression is activated by BMP-4 and regulates inductive tissue interactions in tooth and hair development RL Genes Dev. 10:1382-1394 (1996). RN [14]; RE0014048. RX PUBMED: 9371763. RA Giese K., Pagel J., Grosschedl R. RT Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1 RL Proc. Natl. Acad. Sci. USA 94:12845-12850 (1997). RN [15]; RE0014054. RX PUBMED: 8631802. RA Prieve M. G., Guttridge K. L., Munguia J. E., Waterman M. L. RT The nuclear localization signal of lymphoid enhancer factor-1 is recognized by two differentially expressed Srp1-nuclear localization sequence receptor proteins RL J. Biol. Chem. 271:7654-7658 (1996). RN [16]; RE0014090. RX PUBMED: 9441678. RA McKendry R., Hsu S.-C., Harland R. M., Grosschedl R. RT LEF-1/TCF proteins mediate Wnt-inducible transcription from Xenopus nodal-related 3 promoter RL Dev. Biol. 192:420-431 (1997). RN [17]; RE0014091. RX PUBMED: 9118221. RA Riese J., Yu X., Munnerlyn A., Eresh S., Hsu S.-C., Grosschedl R., Bienz M. RT LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic RL Cell 88:777-787 (1997). RN [18]; RE0014099. RX PUBMED: 10409747. RA Gradl D., Kuhl M., Wedlich D. RT The Wnt/Wg signal transducer beta-catenin controls fibronectin expression RL Mol. Cell. Biol. 19:5576-5587 (1999). RN [19]; RE0014185. RX PUBMED: 8892228. RA Huber O., Korn R., McLaughlin J., Ohsugi M., Herrmann B. G., Kemler R. RT Nuclear localization of beta-catenin by interaction with transcription factor LEF-1 RL Mech. Dev. 59:3-10 (1996). RN [20]; RE0014226. RX PUBMED: 9774110. RA Waltzer L., Bienz M. RT Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling RL Nature 395:521-525 (1998). RN [21]; RE0014336. RX PUBMED: 9671490. RA Hsu S.-C., Galceran J., Grosschedl R. RT Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin RL Mol. Cell. Biol. 18:4807-4818 (1998). RN [22]; RE0014765. RX PUBMED: 10756202. RA Hovanes K., Li T. W. H., Waterman M. L. RT The human LEF-1 gene contains a promoter preferentially active in lymphocytes and encodes multiple isoforms derived from alternative splicing RL Nucleic Acids Res. 28:1994-2003 (2000). RN [23]; RE0014810. RA Pekkanen M., Nieminen P., Sahlberg C., Aberg T., Thesleff I. RT Gene expression in tooth (WWW database); URL: RL Internet : (1996). RN [24]; RE0016625. RX PUBMED: 7774816. RA Giese K., Kingsley C., Kirshner J. R., Grosschedl R. RT Assembly and function of a TCR alpha enhancer complex id dependent on LEF-1-induced DNA bending and multiple protein-protein interactions. RL Genes Dev. 9:995-1008 (1995). XX //