AC T02068
XX
ID T02068
XX
DT 28.01.1997 (created); hiwi.
DT 18.02.2014 (updated); msr.
CO Copyright (C), QIAGEN.
XX
FA PU.1-isoform1
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SY B1; NF-betaA; NF-JB; PU.1; PUrine-box binding factor 1; Spi-1.
XX
OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
XX
GE G005724 SPI1; HGNC: SPI1.
XX
CL C0016; ETS; 3.5.2.5.1.1.
XX
SZ 270 AA; 31.1 kDa (cDNA) (calc.).
XX
SQ MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHS
SQ EFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQ
SQ YPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFLLDLL
SQ RSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGE
SQ VKKVKKKLTYQFSGEVLGRGGLAERRHPPH
XX
SC Swiss-Prot#P17947-1
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FT 1 80 
trans-activation domain, interacting with Rb and TBP [2].
FT 128 265 
DNA binding domain [1].
FT 163 249 
PF00178; Ets-domain.
FT 163 251 
SM00413; etsneu2.
FT 164 247 
PS50061; ETS_DOMAIN_3.
FT 166 238 
PS50140; HSF_ETS.
XX
SF the most divergent member of the ETS family;
SF the glutamine-rich region functions as a trans-activating domain [5];
XX
CP B-cells, macrophages, erythroleukemia cells, spleen, thymus, mast cells, bone marrow, interstitial nonhepatocytes (liver), interstitial nontubular cells (testis).
CN brain, heart, kidney, lung, T cells, fibroblasts, mesothelial cells.
XX
FF activator;
FF after phosphorylation at Ser-148 by CK II, PU.1-isoform1 recruits NF-EM5 to bind to DNA resulting in pronounced transcriptional activation [3] [4];
FF PU.1-isoform1 interferes with the commitment of erythroblast to differentiate;
FF activated by proviral integration of SFFV (anemia- or polycythemia-inducing strains) in 95% of leukemic cell clones;
FF two or three PU.1-isoform1 binding sites cooperate for activation of some myeloid-specific promoters [7];
XX
IN T01412 IRF-4; mouse, Mus musculus.
IN T00722 pRb; human, Homo sapiens.
IN T00794 TBP; human, Homo sapiens.
IN T00795 TBP; fission yeast, Schizosaccharomyces pombe.
IN T00796 TBP; mouse, Mus musculus.
IN T00797 TBP; fruit fly, Drosophila melanogaster.
XX
MX M00971 V$ETS_Q6.
MX M01172 V$PU1_Q4.
MX M00658 V$PU1_Q6.
MX M01203 V$SPI1_01.
MX M02078 V$SPI1_03.
MX M02278 V$SPI1_04.
MX M02031 V$SPI1_Q5.
XX
BS R04050.
BS R04051.
BS R04052.
BS R04049.
BS R02386.
BS R04069.
BS R74002.
BS R20291.
BS R32810.
BS R33634.
BS R20519.
BS R35848.
BS R14490.
BS R04274.
BS R21103.
BS R13565.
BS R01711.
BS R04722.
BS R31083.
BS R35479.
BS R35223.
BS R32811.
BS R32812.
BS R32813.
BS R13568.
BS R13569.
BS R13570.
BS R33520.
BS R33521.
BS R31181.
BS R74007.
BS R31077.
BS R74000.
BS R03475.
BS R08122.
BS R02685.
BS R02982.
BS R01068.
BS R13573.
BS R01155.
BS R02238.
BS R03675.
XX
DR TRANSPATH: MO000011769.
DR TRANSCOMPEL: C00186.
DR TRANSCOMPEL: C00249.
DR TRANSCOMPEL: C00251.
DR TRANSCOMPEL: C00254.
DR TRANSCOMPEL: C00255.
DR TRANSCOMPEL: C00260.
DR TRANSCOMPEL: C00388.
DR TRANSCOMPEL: C00389.
DR EMBL: X52056;
DR UniProtKB: P17947-1;
XX
RN [1]; RE0000109.
RX PUBMED: 2180582.
RA Klemsz M. J., McKercher S. R., Celada A., van Beveren C., Maki R. A.
RT The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene
RL Cell 61:113-124 (1990).
RN [2]; RE0002493.
RX PUBMED: 8434021.
RA Hagemeier C., Bannister A. J., Cook A., Kouzarides T.
RT The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB
RL Proc. Natl. Acad. Sci. USA 90:1580-1584 (1993).
RN [3]; RE0002720.
RX PUBMED: 8456286.
RA Pongubala J. M. R., van Beveren C., Nagulapalli S., Klemsz M. J., McKercher S. R., Maki R. A., Atchison M. L.
RT Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation
RL Science 259:1622-1625 (1993).
RN [4]; RE0003064.
RX PUBMED: 1729611.
RA Pongubala J. M. R., Nagulapalli S., Klemsz M. J., McKercher S. R., Maki R. A., Atchison M. L.
RT PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity
RL Mol. Cell. Biol. 12:368-378 (1992).
RN [5]; RE0005350.
RX PUBMED: 8406004.
RA Shin M. K., Koshland M. E.
RT Ets-related protein PU.1 regulates expression of the immunoglibulin J-chain gene through a novel Ets-binding element
RL Genes Dev. 7:2006-2015 (1993).
RN [6]; RE0005352.
RX PUBMED: 7520173.
RA Voso M. T., Burn T. C., Wulf G., Lim B., Leone G., Tenen D. G.
RT Inhibition of hematopioesis by competitive binding of transcription factor PU.1
RL Proc. Natl. Acad. Sci. USA 91:7932-7936 (1994).
RN [7]; RE0023173.
RX PUBMED: 12036891.
RA Li S.- L., Valente A. J., Qiang M., Schlegel W., Gamez M., Clark R. A.
RT Multiple PU.1 sites cooperate in the regulation of p40(phox) transcription during granulocytic differentiation of myeloid cells.
RL Blood 99:4578-4587 (2002).
XX
//