AC T00722
XX
ID T00722
XX
DT 14.04.1993 (created); ewi.
DT 24.07.2014 (updated); pos.
CO Copyright (C), QIAGEN.
XX
FA pRb
XX
SY p105-RB; pp110; Rb; retinoblastoma tumor suppressor protein; retinoblastoma-associated protein.
XX
OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
XX
GE G001086 RB1; HGNC: RB1.
XX
SF phosphorylation of Thr821 and Thr826 is required to inhibit the binding of LXCXE motif containing proteins (e.g. Elf-1, D-type cyclins) to pRb [38];
SF various phosphorylated forms [4];
SF within hypophosphorylated Rb protein, the domain that interacts with SV40 large T-antigen (T-Ag) also interacts with C/EBPbeta thereby enhancing its DNA-binding and trans-activation [3];
SF the regions 393 - 573 and 645 - 772 are essential and sufficient for complex formation with adenovirus E1A or SV40 large T antigen [8];
XX
CP U2OS [15]; T47D, MCF-7 [12].
CN Saos-2 [15].
XX
FF partially phosphorylated pRb still interacts with E2F and represses the transcription factor [37];
FF tumor suppressor;
FF nonphosphorylated form is supposed to titrate away a negative regulator of Sp1 [4];
FF growth suppression (and E2F binding) requires the pocket domain and the carboxyl terminus (379 - 928) [5];
FF acts as a repressor for p107 gene expression via binding to E2F [6];
FF E2F-1, -2, -3 bind preferentially and E2F-4 (to a lesser degree) to pRb [7] [9];
FF is not detectably associated with E2F during T-cell G1 phase [7];
XX
IN T01795 AhR; human, Homo sapiens.
IN T08443 AML3; mouse, Mus musculus.
IN T00167 ATF-2-isoform1; human, Homo sapiens.
IN T05451 BRG1; human, Homo sapiens.
IN T16987 BRG1; Mammalia.
IN T00140 c-Myc-isoform1; human, Homo sapiens.
IN T00581 C/EBPbeta; human, Homo sapiens.
IN T04197 CLIM2; human, Homo sapiens.
IN T06570 Dnmt1; human, Homo sapiens.
IN T00967 E1A 12S protein; adenovirus.
IN T00209 E1A 13S protein; adenovirus type 2.
IN T15605 E2A; human, Homo sapiens.
IN T01542 E2F-1; human, Homo sapiens.
IN T09117 E2F-1; human, Homo sapiens.
IN T01544 E2F-2; human, Homo sapiens.
IN T14606 E2F-3; human, Homo sapiens.
IN T01546 E2F-4; human, Homo sapiens.
IN T00221 E2F:DP; human, Homo sapiens.
IN T01376 E7; HPV-16, human papilloma virus type 16.
IN T01113 Elf-1; human, Homo sapiens.
IN T04106 HDAC1; human, Homo sapiens.
IN T25625 hdac2; human, Homo sapiens.
IN T04110 HDAC3; human, Homo sapiens.
IN T09895 HES-1; human, Homo sapiens.
IN T01212 Id2; human, Homo sapiens.
IN T34277 mcm7; human, Homo sapiens.
IN T02286 MTB-Zf; human, Homo sapiens.
IN T00526 MyoD; mouse, Mus musculus.
IN T00520 Myogenin; human, Homo sapiens.
IN T01445 N-Myc; mouse, Mus musculus.
IN T09420 neurofibromin1; human, Homo sapiens.
IN T34034 p21Cip1; human, Homo sapiens.
IN T02336 PLZF; human, Homo sapiens.
IN T02068 PU.1-isoform1; human, Homo sapiens.
IN T00702 PU.1; mouse, Mus musculus.
IN T00703 PU.1; chick, Gallus gallus.
IN T27214 RBBP1; human, Homo sapiens.
IN T01489 RBP60; human, Homo sapiens.
IN T02250 RBTN2-isoform1; human, Homo sapiens.
IN T00759 Sp1; human, Homo sapiens.
IN T00788 T-Ag; SV40, simian virus 40.
IN T00781 TAFII250-isoform2; human, Homo sapiens.
IN T19290 Tal-1; human, Homo sapiens.
IN T11343 TBX2; mouse, Mus musculus.
IN T02183 TFIIH-p62; human, Homo sapiens.
XX
BS R39267.
XX
DR TRANSPATH: MO000017774.
DR UniProtKB: P06400;
XX
RN [1]; RE0002493.
RX PUBMED: 8434021.
RA Hagemeier C., Bannister A. J., Cook A., Kouzarides T.
RT The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB
RL Proc. Natl. Acad. Sci. USA 90:1580-1584 (1993).
RN [2]; RE0003304.
RX PUBMED: 8264596.
RA Shan B., Chang C. Y., Jones D., Lee W. H.
RT The transcription factor E2F-1 mediates the autoregulation of RB gene expression
RL Mol. Cell. Biol. 14:299-309 (1994).
RN [3]; RE0006633.
RX PUBMED: 8552662.
RA Chen P.-L., Riley D. J., Chen-Kiang S., Lee W.-H.
RT Retinoblastoma protein directly interacts with and activates the transcription factor NF-IL6
RL Proc. Natl. Acad. Sci. USA 93:465-469 (1996).
RN [4]; RE0006713.
RX PUBMED: 8007947.
RA Chen L. I., Nishinaka T., Kwan K., Kitabayashi I., Yokoyama K., Fu Y.-H. F., Gruenwald S., Chiu R.
RT The retinoblastoma gene product RB stimulates Sp1-mediated transcription by liberating Sp1 from a negative regulator
RL Mol. Cell. Biol. 14:4380-4389 (1994).
RN [5]; RE0009524.
RX PUBMED: 1534305.
RA Qin X.-q., Chittenden T., Livingston D. M., Kaelin jr W. G.
RT Identification of a growth suppression domain within the retinoblastoma gene product
RL Genes Dev. 6:953-964 (1992).
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RA Zhu L., Zhu L., Xie E., Chang L.-S.
RT Differential roles of two tandem E2F sites in repression of the human p107 promoter by retinoblastoma and p107 proteins
RL Mol. Cell. Biol. 15:3552-3562 (1995).
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RX PUBMED: 8657117.
RA Moberg K., Starz M. A., Lees J. A.
RT E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry
RL Mol. Cell. Biol. 16:1436-1449 (1996).
RN [8]; RE0013301.
RX PUBMED: 2138977.
RA Hu Q. J., Dyson N., Harlow E.
RT The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations.
RL EMBO J. 9:1147-1155 (1990).
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RA Kohn K. W.
RT Molecular interaction map of the mammalian cell cycle control and DNA repair systems
RL Mol. Biol. Cell 10:2703-2734 (1999).
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RX PUBMED: 12386167.
RA Chen Y., Riley D. J., Zheng L., Chen P.-L., Lee W.-H.
RT Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation.
RL J. Biol. Chem. 277:49408-49416 (2002).
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RX PUBMED: 9652748.
RA Fiddes R. J., Janes P. W., Sivertsen S. P., Sutherland R. L., Musgrove E. A., Daly R. J.
RT Inhibition of the MAP kinase cascade blocks heregulin-induced cell cycle progression in T-47D human breast cancer cells.
RL Oncogene 16:2803-13 (1998).
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RX PUBMED: 11896613.
RA Singh J., Murata K., Itahana Y., Desprez P. Y.
RT Constitutive expression of the Id-1 promoter in human metastatic breast cancer cells is linked with the loss of NF-1/Rb/HDAC-1 transcription repressor complex.
RL Oncogene 21:1812-22 (2002).
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RX PUBMED: 12397079.
RA Pardo P. S., Leung J. K., Lucchesi J. C., Pereira-Smith O. M.
RT MRG15, a novel chromodomain protein, is present in two distinct multiprotein complexes involved in transcriptional activation
RL J. Biol. Chem. 277:50860-6 (2002).
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RX PUBMED: 9256431.
RA Chang K. H., Chen Y., Chen T. T., Chou W. H., Chen P. L., Ma Y. Y., Yang-Feng T. L., Leng X., Tsai M. J., O'Malley B. W., Lee W. H.
RT A thyroid hormone receptor coactivator negatively regulated by the retinoblastoma protein
RL Proc. Natl. Acad. Sci. USA 94:9040-5 (1997).
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RX PUBMED: 16753023.
RA Lee J. S., Thomas D. M., Gutierrez G., Carty S. A., Yanagawa S., Hinds P. W.
RT HES1 cooperates with pRb to activate RUNX2-dependent transcription.
RL J. Bone Miner. Res. 21:921-933 (2006).
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RX PUBMED: 16407216.
RA Tu Z., Prajapati S., Park K. J., Kelly N. J., Yamamoto Y., Gaynor R. B.
RT IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression.
RL J. Biol. Chem. 281:6699-6706 (2006).
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RX PUBMED: 11073990.
RA MacLellan W. R., Xiao G., Abdellatif M., Schneider M. D.
RT A novel Rb- and p300-binding protein inhibits transactivation by MyoD.
RL Mol. Cell. Biol. 20:8903-8915 (2000).
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RA Munakata T., Nakamura M., Liang Y., Li K., Lemon S. M.
RT Down-regulation of the retinoblastoma tumor suppressor by the hepatitis C virus NS5B RNA-dependent RNA polymerase.
RL Proc. Natl. Acad. Sci. USA 102:18159-18164 (2005).
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RA Khwaja F., Tabassum A., Allen J., Djakiew D.
RT The p75(NTR) tumor suppressor induces cell cycle arrest facilitating caspase mediated apoptosis in prostate tumor cells.
RL Biochem. Biophys. Res. Commun. 341:1184-1192 (2006).
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RX PUBMED: 10490602.
RA Lai A., Lee J. M., Yang W. M., DeCaprio J. A., Kaelin WG J. r., Seto E., Branton P. E.
RT RBP1 recruits both histone deacetylase-dependent and -independent repression activities to retinoblastoma family proteins.
RL Mol. Cell. Biol. 19:6632-6641 (1999).
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RX PUBMED: 17468517.
RA Saegusa M., Hashimura M., Kuwata T., Hamano M., Wani Y., Okayasu I.
RT A functional role of Cdx2 in beta-catenin signaling during transdifferentiation in endometrial carcinomas.
RL Carcinogenesis 28:1885-1892 (2007).
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RA Lobenhofer E. K., Huper G., Iglehart J. D., Marks J. R.
RT Inhibition of mitogen-activated protein kinase and phosphatidylinositol 3-kinase activity in MCF-7 cells prevents estrogen-induced mitogenesis.
RL Cell Growth Differ. 11:99-110 (2000).
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RA Balasenthil S., Vadlamudi R. K.
RT Functional interactions between the estrogen receptor coactivator PELP1/MNAR and retinoblastoma protein.
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RX PUBMED: 10888886.
RA Robertson K. D., Ait-Si-Ali S., Yokochi T., Wade P. A., Jones P. L., Wolffe A. P.
RT DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters.
RL Nat. Genet. 25:338-342 (2000).
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RA Muraoka-Cook R. S., Caskey L. S., Sandahl M. A., Hunter D. M., Husted C., Strunk K. E., Sartor C. I., Rearick WA J. r., McCall W., Sgagias M. K., Cowan K. H., Earp HS 3. r. d.
RT Heregulin-dependent delay in mitotic progression requires HER4 and BRCA1.
RL Mol. Cell. Biol. 26:6412-6424 (2006).
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RA Fernando R., Foster J. S., Bible A., Strom A., Pestell R. G., Rao M., Saxton A., Baek S. J., Yamaguchi K., Donnell R., Cekanova M., Wimalasena J.
RT Breast cancer cell proliferation is inhibited by BAD: regulation of cyclin D1.
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RA Araki K., Kawauchi K., Tanaka N.
RT IKK/NF-kappaB signaling pathway inhibits cell-cycle progression by a novel Rb-independent suppression system for E2F transcription factors.
RL Oncogene 27:5696-5705 (2008).
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RT IFN-gamma inhibits human airway smooth muscle cell proliferation by modulating the E2F-1/Rb pathway.
RL Am. J. Physiol. Lung Cell. Mol. Physiol. 284:L1063-71 (2003).
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RA Boiko A. D., Porteous S., Razorenova O. V., Krivokrysenko V. I., Williams B. R., Gudkov A. V.
RT A systematic search for downstream mediators of tumor suppressor function of p53 reveals a major role of BTG2 in suppression of Ras-induced transformation.
RL Genes Dev. 20:236-252 (2006).
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RA Sun J. M., Chen H. Y., Davie J. R.
RT Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin.
RL J. Biol. Chem. 282:33227-33236 (2007).
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RX PUBMED: 20220142.
RA Por E., Byun H. J., Lee E. J., Lim J. H., Jung S. Y., Park I., Kim Y. M., Jeoung D. I., Lee H.
RT The cancer/testis antigen CAGE with oncogenic potential stimulates cell proliferation by up-regulating cyclins D1 and E in an AP-1- and E2F-dependent manner.
RL J. Biol. Chem. 285:14475-14485 (2010).
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RT MicroRNA-34a inhibits uveal melanoma cell proliferation and migration through downregulation of c-Met.
RL Invest. Ophthalmol. Vis. Sci. 50:1559-1565 (2009).
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RT NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.
RL Nature 466:1125-1128 (2010).
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RA Medunjanin S., Weinert S., Schmeisser A., Mayer D., Braun-Dullaeus R. C.
RT Interaction of the double-strand break repair kinase DNA-PK and estrogen receptor-alpha.
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RA Vance K. W., Shaw H. M., Rodriguez M., Ott S., Goding C. R.
RT The retinoblastoma protein modulates Tbx2 functional specificity.
RL Mol. Biol. Cell 21:2770-2779 (2010).
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RA Lin C. Y., Tan B. C., Liu H., Shih C. J., Chien K. Y., Lin C. L., Yung B. Y.
RT Dephosphorylation of nucleophosmin by PP1beta facilitates pRB binding and consequent E2F1-dependent DNA repair.
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RA Lundberg A. S., Weinberg R. A.
RT Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes
RL Mol. Cell. Biol. 18:753-761 (1998).
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RA Knudsen E. S., Wang J. Y. J.
RT Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites
RL J. Biol. Chem. 271:8313-8320 (1996).
XX
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