AC T00781
XX
ID T00781
XX
DT 14.04.1993 (created); ewi.
DT 30.12.2013 (updated); yre.
CO Copyright (C), QIAGEN.
XX
FA TAFII250-isoform2
XX
SY CCG1; p250; TAF-250; TAFII250; TBP-associated factor 250.
XX
OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
XX
GE G009204 TAF1; HGNC: TAF1.
XX
CL C0015; HMG; 4.1.3.0.5.2.
XX
SZ 1893 AA; 214.7 kDa (cDNA) (calc.), 250 kDa (SDS) [13] [15] [9]
XX
SQ MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDD
SQ ECKKHLAGLGALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDE
SQ SRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGVSE
SQ NGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEATQAESEDGKLTLPLAGIMQHDA
SQ TKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVE
SQ CSVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVA
SQ EWRYGPARLWYDMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLAD
SQ ENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLD
SQ DDKPWYSIFPIDNEDLVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEE
SQ ATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQG
SQ LRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPL
SQ LKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATK
SQ IKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPE
SQ TDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSK
SQ DRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAM
SQ VSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFI
SQ AAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLS
SQ LKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQ
SQ ERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSR
SQ EREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEY
SQ VRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQE
SQ KEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQ
SQ EEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVG
SQ TTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYK
SQ IITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDE
SQ KLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPD
SQ YYKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNV
SQ CYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMS
SQ RDASVFQDESNMSVLDIPSATPEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMS
SQ EGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESM
SQ MSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRS
SQ GPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE
XX
SC PIR #A40262
XX
FT 1 177 
NF-kappaB homology region [15].
FT 146 372 
missing in TAF(II)250delta [14].
FT 178 198 
missing in CCG1 [14].
FT 514 1022 
PF00478; IMP dehydrogenase / GMP reductase domain.
FT 692 707 
sigma4.1 homology region [15].
FT 987 1167 
SWI4 homology region [15].
FT 1021 1031 
sigma2.1 homology region [15].
FT 1141 1231 
binding of TFIIF-alpha [20].
FT 1232 1260 
sigma4.2 homology region [15].
FT 1399 1507 
SM00297; bromo_6.
FT 1406 1493 PF00439; Bromodomain.
FT 1418 1488 PS50014; BROMODOMAIN_2.
FT 1521 1630 SM00297; bromo_6.
FT 1528 1616 PF00439; Bromodomain.
FT 1541 1611 PS50014; BROMODOMAIN_2.
XX
SF TAFII250-isoform2, TAFII100 and TAFII28 are human coreTAF(II)s common to both TFIIDalpha and TFIIDbeta complexes [22];
SF probable splice variants are CCG1 T02206 and TAFII250-isoform2Delta T02207 [14];
SF part of the human TFIID (D-TFIID) complex [3];
SF interacting with the conserved C-terminal part of TBP where the second direct repeat is of particular importance [9] [14];
SF sequence similarity with NF-kappaB1 p105, position 733-900: 22% identity, 39% similarity [15];
SF homology with cell-cycle regulating yeast SWI4: 21% identity, 36% similarity [15];
SF part of the two direct repeats are two bromo domains;
XX
FF tight association with TBP, role in assembly of the TFIID complex;
FF its interaction with TBP correlates with ability of TFIID to confer activation by upstream factors [7];
FF essential for cell cycle progression through G1 phase [19];
XX
IN T00671 p53; human, Homo sapiens.
IN T00722 pRb; human, Homo sapiens.
IN T00784 TAFII100; human, Homo sapiens.
IN T02328 TAFII135; human, Homo sapiens.
IN T02118 TAFII30; human, Homo sapiens.
IN T00782 TAFII55; human, Homo sapiens.
IN T09238 TAFII55; human, Homo sapiens.
IN T00783 TAFII70-alpha; human, Homo sapiens.
IN T02208 TAFII70-gamma; human, Homo sapiens.
IN T00794 TBP; human, Homo sapiens.
IN T00797 TBP; fruit fly, Drosophila melanogaster.
IN T02168 TFIIF-alpha; human, Homo sapiens.
IN T01038 TFIIF; human, Homo sapiens.
XX
DR TRANSPATH: MO000025167.
DR EMBL: D90359;
DR EMBL: X07024;
DR UniProtKB: P21675-2;
XX
RN [1]; RE0000746.
RX PUBMED: 1398073.
RA Zhou Q., Lieberman P. M., Boyer T. G., Berk A. J.
RT Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter
RL Genes Dev. 6:1964-1974 (1992).
RN [2]; RE0002494.
RX PUBMED: 1465404.
RA Takada R., Nakatani Y., Hoffmann A., Kokubo T., Hasegawa S., Roeder R. G., Horikoshi M.
RT Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIIDtau)
RL Proc. Natl. Acad. Sci. USA 89:11809-11813 (1992).
RN [3]; RE0002495.
RX PUBMED: 1387711.
RA Timmers H. T. M., Meyers R. E., Sharp P. A.
RT Composition of transcription factor B-TFIID
RL Proc. Natl. Acad. Sci. USA 89:8140-8144 (1992).
RN [4]; RE0005507.
RX PUBMED: 8758937.
RA Dubrovskaya V., Lavigne A.-C., Davidson I., Acker J., Staub A., Tora L.
RT Distinct domains of hTAFII100 are requireed for functional interactions with transcription factor TFIIbeta (RAP30) and incorporation into the TFIID complex
RL EMBO J. 15:3702-3712 (1996).
RN [5]; RE0005525.
RX PUBMED: 7724524.
RA Shao Z., Ruppert S., Robbins P. D.
RT The retinoblastoma-susceptibility gene product binds directly to the human TATA-binding protein-associated factor TAFII250
RL Proc. Natl. Acad. Sci. USA 92:3115-3119 (1995).
RN [6]; RE0005526.
RX PUBMED: 7824954.
RA Chiang C.-M., Roeder R. G.
RT Cloning of an instrinsic human TFIID subunit that interacts with multiple transcriptional activators
RL Science 267:531-536 (1995).
RN [7]; RE0005555.
RX PUBMED: 7958931.
RA Tansey W. P., Ruppert S., Tjian R., Herr W.
RT Multiple regions of TBP participate in the response to transcriptional activators in vivo
RL Genes Dev. 8:2756-2769 (1994).
RN [8]; RE0005557.
RX PUBMED: 1657708.
RA Pugh B. F., Tjian R.
RT Transcription from a TATA-less promoter requires a multisubunit TFIID complex
RL Genes Dev. 5:1935-1945 (1991).
RN [9]; RE0005559.
RX PUBMED: 8436290.
RA Zhou Q., Boyer T. G., Berk A. J.
RT Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain
RL Genes Dev. 7:180-187 (1993).
RN [10]; RE0005560.
RX PUBMED: 8262073.
RA Weinzierl R. O. J., Ruppert S., Dynlacht B. D., Tanese N., Tjian R.
RT Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID
RL EMBO J. 12:5303-5309 (1993).
RN [11]; RE0005562.
RX PUBMED: 7923369.
RA Jacq X., Brou C., Lutz Y., Davidson I., Chambon P., Tora L.
RT Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor
RL Cell 79:107-117 (1994).
RN [12]; RE0005568.
RX PUBMED: 8464492.
RA Weinzierl R. O. J., Dynlacht B. D., Tjian R.
RT Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator
RL Nature 362:511-517 (1993).
RN [13]; RE0005583.
RX PUBMED: 8183347.
RA Kim T. K., Hashimoto S., Kelleher III R. J., Flanagan P. M., Kornberg R. D., Horikoshi M., Roeder R. G.
RT Effects of activation-defective TBP mutations on transcription initiation in yeast
RL Nature 369:252-255 (1994).
RN [14]; RE0005584.
RX PUBMED: 7680771.
RA Ruppert S., Wang E. H., Tjian R.
RT Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation
RL Nature 362:175-179 (1993).
RN [15]; RE0005585.
RX PUBMED: 8450888.
RA Hisatake K., Hasegawa S., Takada R., Nakatani Y., Horikoshi M., Roeder R. G.
RT The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1
RL Nature 362:179-181 (1993).
RN [16]; RE0005586.
RX PUBMED: 3169001.
RA Sekiguchi T., Miyata T., Nishimoto T.
RT Molecular cloning of the cDNA of human X chromosomal gene (CCG1) which complement the temperature sensitive G1 mutants, tsBN462 and ts13 of BHK cells
RL EMBO J. 7:1683-1687 (1988).
RN [17]; RE0005587.
RX PUBMED: 1350857.
RA Haynes S. R., Dollard C., Winston F., Beck S., Trowsdale J., Dawid I. B.
RT The bromodomain: A conserved sequence found in human, Drosophila and yeast proteins
RL Nucleic Acids Res. 20:2603-2603 (1992).
RN [18]; RE0005588.
RX PUBMED: 2038334.
RA Sekiguchi T., Nohiro Y., Nakamura Y., Hisamoto N., Nishimoto T.
RT The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein
RL Mol. Cell. Biol. 11:3317-3325 (1991).
RN [19]; RE0005589.
RX PUBMED: 8303298.
RA Wang E., Tjian R.
RT Promoter-selective transcriptional defect in cell cycle mutant ts13 rescued by hTAFII250
RL Science 263:811-814 (1994).
RN [20]; RE0005590.
RX PUBMED: 7590250.
RA Ruppert S., Tjian R.
RT Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation
RL Genes Dev. 9:2747-2755 (1995).
RN [21]; RE0047697.
RX PUBMED: 15988478.
RA Kim T. H., Barrera L. O., Zheng M., Qu C., Singer M. A., Richmond T. A., Wu Y., Green R. D., Ren B.
RT A high-resolution map of active promoters in the human genome.
RL Nature 436:876-880 (2005).
RN [22]; RE0005528.
RX PUBMED: 7729427.
RA Mengus G., May M., Jacq X., Staub A., Tora L., Chambon P., Davidson I.
RT Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID
RL EMBO J. 14:1520-1531 (1995).
XX
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