AC T01542
XX
ID T01542
XX
DT 07.08.1995 (created); ewi.
DT 25.05.2016 (updated); nes.
CO Copyright (C), QIAGEN.
XX
FA E2F-1
XX
SY adenovirus E2 gene binding Factor 1; E2F transcription factor 1; E2F-1; E2F1; RBB3; RBBP3; RBP3.
XX
OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
XX
GE G001150 E2F1; HGNC: E2F1.
XX
CL C0023; fork head; 3.3.2.1.1.
XX
SF homo- and heterodimerizes, C (= E2F activity) show enhanced DNA- and Rb-binding;
SF heterodimerization with DP-1 depends on an intact zipper motif [8];
SF prior to crystal structure belongs to bHLH-zip factors [20];
SF also binds cyclinA/cdk2, but not the individual subunits [7];
SF the Rb-binding region also contacts TBP [6];
XX
CP SkBr3, MCF-7, MDA-MB-468, MDA-MB-231, NBE018, NBE234, NBE232B, NBE233 [34].
XX
FF E2F-1 contributes the transcriptional activation domain to E2F-1/DP-1 heterodimers (E2F) [3] [4];
FF overexpression may cause neoplastic transformation [11];
FF deregulated function in cooperation with ras causes oncogenic transformation [17];
FF autoregulates its own promoter in a cell cycle-dependent manner [9] [13] [15];
FF binding of Rb masks the transcription activation region and prevents it from interacting with TBP [6];
FF phosphorylation at Ser-332 and Ser-337 at the end of G1 phase, presumably by cdc2 or cdk2, inhibits Rb binding and enhances interaction with adenoviral E4 [10];
FF cyclin A/cdk2 binds E2F-1 and inhibits its DNA-binding by phosphorylation [7] [12] [16];
FF no phosphorylation by cyclin E/cdk2 [12];
FF expression of E2F-1 activity is lost upon senescence of cells [14];
FF E2F-1 regulates the expression of Apaf-1, the gene for apoptosis protease-activating factor 1;
FF provides a direct link between the deregulation of the pRB pathway and apoptosis [21];
FF expression is up-regulated in G1/S phase transition and during S phase in T-cells, where E2F-1 is predominantly present in a free form without bound regulatory protein (pRb) [18];
XX
IN T15601 AR; Mammalia.
IN T03240 DDB; human, Homo sapiens.
IN T01548 DP-1; human, Homo sapiens.
IN T01549 DP-1; mouse, Mus musculus.
IN T03000 DP-2; human, Homo sapiens.
IN T01542 E2F-1; human, Homo sapiens.
IN T01544 E2F-2; human, Homo sapiens.
IN T23419 MCT-isoform1; human, Homo sapiens.
IN T25652 MCT; human, Homo sapiens.
IN T06148 p/CAF; human, Homo sapiens.
IN T25811 p107-isoform1; human, Homo sapiens.
IN T00671 p53; human, Homo sapiens.
IN T00722 pRb; human, Homo sapiens.
IN T08513 pRb; human, Homo sapiens.
IN T25008 pRb; Mammalia.
IN T08711 RelA-p65; human, Homo sapiens.
IN T00794 TBP; human, Homo sapiens.
IN T02183 TFIIH-p62; human, Homo sapiens.
IN T27164 Tip60; human, Homo sapiens.
IN T05140 trrap; human, Homo sapiens.
XX
MX M01250 V$E2F1_01.
MX M01251 V$E2F1_02.
MX M04746 V$E2F1_06.
MX M04895 V$E2F1_07.
MX M07206 V$E2F1_09.
MX M00428 V$E2F1_Q3.
MX M00938 V$E2F1_Q3_01.
MX M00430 V$E2F1_Q4.
MX M07250 V$E2F1_Q4_01.
MX M08874 V$E2F1_Q4_02.
MX M00431 V$E2F1_Q6.
MX M00024 V$E2F_01.
MX M00050 V$E2F_02.
MX M00516 V$E2F_03.
MX M00803 V$E2F_Q2.
MX M00918 V$E2F_Q3_01.
MX M00919 V$E2F_Q4_01.
MX M00939 V$E2F_Q4_02.
MX M08875 V$E2F_Q4_03.
MX M00920 V$E2F_Q6_01.
XX
BS R22101.
BS R61766.
BS R04555.
BS R73983.
BS R33988.
BS R62781.
BS R30591.
BS R70785.
BS R70786.
BS R57951.
BS R57955.
BS R57956.
BS R57957.
BS R57959.
BS R21009.
BS R59630.
BS R30885.
BS R60817.
BS R41214.
BS R41215.
BS R60137.
BS R04349.
BS R25316.
BS R57567.
BS R09600.
BS R09601.
BS R66646.
BS R25508.
BS R31205.
BS R35293.
BS R58991.
BS R71060.
BS R66296.
BS R04352.
BS R04353.
BS R25950.
BS R71996.
BS R24728.
BS R69920.
BS R66274.
BS R55832.
BS R66187.
BS R62086.
BS R04381.
BS R04382.
BS R61209.
BS R61210.
BS R61211.
BS R61212.
BS R61718.
BS R66654.
BS R55929.
BS R55934.
BS R63798.
BS R63513.
BS R25607.
BS R09798.
BS R63494.
BS R33986.
BS R33987.
BS R37461.
BS R38032.
BS R61191.
BS R61195.
BS R61198.
BS R61201.
BS R38719.
BS R70421.
BS R70636.
BS R71982.
BS R71983.
BS R71984.
BS R71986.
BS R71987.
BS R71988.
BS R71989.
BS R71990.
BS R71991.
BS R71992.
BS R71993.
BS R71977.
BS R71979.
BS R71980.
BS R66487.
BS R59914.
BS R71356.
BS R59576.
BS R71357.
BS R67974.
BS R39260.
BS R68759.
BS R68760.
BS R68761.
BS R68762.
BS R68763.
BS R61215.
BS R61216.
BS R61217.
BS R61218.
BS R61219.
BS R04386.
BS R66414.
BS R66426.
BS R65063.
BS R65065.
BS R65067.
BS R58893.
BS R67203.
BS R70418.
BS R66376.
BS R66380.
BS R22203.
BS R25714.
BS R25717.
BS R64950.
BS R61545.
BS R66455.
BS R66456.
BS R66457.
BS R24471.
BS R71215.
BS R71271.
BS R62594.
BS R62596.
BS R21646.
BS R65321.
BS R09599.
BS R25484.
BS R64240.
BS R64241.
BS R64244.
XX
DR TRANSPATH: MO000004274.
DR TRANSCOMPEL: C00226.
DR TRANSCOMPEL: C00580.
DR UniProtKB: Q01094;
XX
RN [1]; RE0000163.
RX PUBMED: 1828392.
RA Chellappan S. P., Hiebert S., Mudryj M., Horowitz J. M., Nevins J. R.
RT The E2F transcription factor is a cellular target for the RB protein
RL Cell 65:1053-1061 (1991).
RN [2]; RE0001236.
RX PUBMED: 2523514.
RA Yee A. S., Raychaudhuri P., Jakoi L., Nevins J. R.
RT The Adenovirus-Inducible Factor E2F Stimulates Transcription after Specific DNA Binding
RL Mol. Cell. Biol. 9:578-585 (1989).
RN [3]; RE0003281.
RX PUBMED: 1638635.
RA Kaelin W. G., Krek W., Sellers W. R., DeCaprio J. A., Ajchenbaum F., fuchs C. S., Chittenden T., Blanar M. A., Livingston D. M., Flemington E. K.
RT Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties
RL Cell 70:351-364 (1992).
RN [4]; RE0003282.
RX PUBMED: 1638634.
RA Helin K., Lees J. A., Vidal M., Dyson N., Fattaey A.
RT A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F
RL Cell 70:337-350 (1992).
RN [5]; RE0003284.
RX PUBMED: 1448092.
RA Shan B., Zhu X., Chen P.-L., Durfee T., Yang Y., Sharp D., Lee W.-H.
RT Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: identification of a gene with properties of the transcription factor E2F
RL Mol. Cell. Biol. 12:5620-5631 (1992).
RN [6]; RE0003294.
RX PUBMED: 8255752.
RA Hagemeier C., Cook A., Kouzarides T.
RT The retinoblastoma protein binds E2F residues required for activation in vivo and TBP binding in vitro
RL Nucleic Acids Res. 21:4998-5004 (1993).
RN [7]; RE0003299.
RX PUBMED: 7969176.
RA Xu M., Sheppard K. A., Peng C.-Y., Yee A. S., Piwnica-Worms H.
RT Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation
RL Mol. Cell. Biol. 14:8420-8431 (1994).
RN [8]; RE0003300.
RX PUBMED: 8248803.
RA Krek W., Livingston D. M., Shirodkar S.
RT Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members
RL Science 262:1557-1560 (1993).
RN [9]; RE0003306.
RX PUBMED: 7935380.
RA Neuman E., Flemington E. K., Sellers W. R., Kaelin jr W. G.
RT Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter
RL Mol. Cell. Biol. 14:6607-6615 (1994).
RN [10]; RE0003308.
RX PUBMED: 8087847.
RA Fagan R., Flint K. J., Jones N.
RT Phosphorylation of E2F-1 modulates its interaction with the retinoblastoma gene product and the adenoviral E4 19 kDa protein
RL Cell 78:799-811 (1994).
RN [11]; RE0003309.
RX PUBMED: 8045262.
RA Singh P., Wong S. H., Hong W.
RT Overexpression of E2F-1 in rat embryo fibroblasts leads to neoplastic transformation
RL EMBO J. 13:3329-3338 (1994).
RN [12]; RE0003310.
RX PUBMED: 7958856.
RA Dynlacht B. D., Flores O., Lees J. A., Harlow E.
RT Differential regulation of E2F trans-activation by cyclin/cdk2 complexes
RL Genes Dev. 8:1772-1786 (1994).
RN [13]; RE0003311.
RX PUBMED: 7958837.
RA Hsiao K. M., McMahon S. L., Farnham P. J.
RT Multiple DNA elements are required for the growth regulation of the mouse E2F1 promoter
RL Genes Dev. 8:1526-1537 (1994).
RN [14]; RE0003312.
RX PUBMED: 8206919.
RA Dimri G. P., Hara E., Campisi J.
RT Regulation of two E2F-related genes in presenescent and senescent human fibroblasts
RL J. Biol. Chem. 269:16180-16186 (1994).
RN [15]; RE0003313.
RX PUBMED: 7958836.
RA Johnson D. G., Ohtani K., Nevins J. R.
RT Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression
RL Genes Dev. 8:1514-1525 (1994).
RN [16]; RE0003314.
RX PUBMED: 8033208.
RA Krek W., Ewen M. E., Shirodkar S., Arany Z., Kaelin jr W. G., Livingston D. M.
RT Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinase
RL Cell 78:161-172 (1994).
RN [17]; RE0003545.
RX PUBMED: 7809128.
RA Johnson D. G., Cress W. D., Jakoi L., Nevins J. R.
RT Oncogenic capacity of the E2F1 gene
RL Proc. Natl. Acad. Sci. USA 91:12823-12827 (1994).
RN [18]; RE0012605.
RX PUBMED: 8657117.
RA Moberg K., Starz M. A., Lees J. A.
RT E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry
RL Mol. Cell. Biol. 16:1436-1449 (1996).
RN [19]; RE0014167.
RX PUBMED: 9418871.
RA Hayes S., Shiyanov P., Chen X., Raychaudhuri P.
RT DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1
RL Mol. Cell. Biol. 18:240-249 (1998).
RN [20]; RE0015322.
RX PUBMED: 10090723.
RA Zheng N., Fraenkel E., Pabo C. O., Pavletich N. P.
RT Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DP
RL Genes Dev. 13:666-674 (1999).
RN [21]; RE0016344.
RX PUBMED: 11389439.
RA Moroni M. C., Hickman E. S., Denchi E. L., Caprara G., Colli E., Cecconi F., Muller H., Helin K.
RT Apaf-1 is a transcriptional target for E2F and p53.
RL Nat. Cell Biol. 3:552-558 (2001).
RN [22]; RE0020169.
RX PUBMED: 8964493.
RA NEUMAN E., SELLERS W.R.S., MCNEIL J.A., LAWRENCE J.B., KAELIN W.G. JR.
RT Structure and partial genomic sequence of the human E2F1 gene.
RL Gene 173:163-169 (1996).
RN [23]; RE0037431.
RX PUBMED: 10675335.
RA Martinez-Balbas M. A., Bauer U. M., Nielsen S. J., Brehm A., Kouzarides T.
RT Regulation of E2F1 activity by acetylation
RL EMBO J. 19:662-71 (2000).
RN [24]; RE0048291.
RX PUBMED: 16938873.
RA El Messaoudi S., Fabbrizio E., Rodriguez C., Chuchana P., Fauquier L., Cheng D., Theillet C., Vandel L., Bedford M. T., Sardet C.
RT Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene.
RL Proc. Natl. Acad. Sci. USA 103:13351-13356 (2006).
RN [25]; RE0048940.
RX PUBMED: 16135794.
RA Datta A., Sen J., Hagen J., Korgaonkar C. K., Caffrey M., Quelle D. E., Hughes D. E., Ackerson T. J., Costa R. H., Raychaudhuri P.
RT ARF directly binds DP1: interaction with DP1 coincides with the G1 arrest function of ARF.
RL Mol. Cell. Biol. 25:8024-8036 (2005).
RN [26]; RE0048950.
RX PUBMED: 17050006.
RA Garcia-Alvarez G., Ventura V., Ros O., Aligue R., Gil J., Tauler A.
RT Glycogen synthase kinase-3beta binds to E2F1 and regulates its transcriptional activity.
RL Biochim. Biophys. Acta 1773:375-382 (2006).
RN [27]; RE0049004.
RX PUBMED: 16407216.
RA Tu Z., Prajapati S., Park K. J., Kelly N. J., Yamamoto Y., Gaynor R. B.
RT IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression.
RL J. Biol. Chem. 281:6699-6706 (2006).
RN [28]; RE0049543.
RX PUBMED: 15123636.
RA Ianari A., Gallo R., Palma M., Alesse E., Gulino A.
RT Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage.
RL J. Biol. Chem. 279:30830-30835 (2004).
RN [29]; RE0049544.
RX PUBMED: 10965846.
RA Denis G. V., Vaziri C., Guo N., Faller D. V.
RT RING3 kinase transactivates promoters of cell cycle regulatory genes through E2F.
RL Cell Growth Differ. 11:417-424 (2000).
RN [30]; RE0050272.
RX PUBMED: 16460673.
RA Khwaja F., Tabassum A., Allen J., Djakiew D.
RT The p75(NTR) tumor suppressor induces cell cycle arrest facilitating caspase mediated apoptosis in prostate tumor cells.
RL Biochem. Biophys. Res. Commun. 341:1184-1192 (2006).
RN [31]; RE0052226.
RX PUBMED: 17006541.
RA Liu K., Paik J. C., Wang B., Lin F. T., Lin W. C.
RT Regulation of TopBP1 oligomerization by Akt/PKB for cell survival.
RL EMBO J. 25:4795-4807 (2006).
RN [32]; RE0052874.
RX PUBMED: 17912037.
RA del Arroyo A. G., El Messaoudi S., Clark P. A., James M., Stott F., Bracken A., Helin K., Peters G.
RT E2F-dependent induction of p14ARF during cell cycle re-entry in human T cells.
RL Cell Cycle 6:2697-2705 (2007).
RN [33]; RE0053042.
RX PUBMED: 10888886.
RA Robertson K. D., Ait-Si-Ali S., Yokochi T., Wade P. A., Jones P. L., Wolffe A. P.
RT DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters.
RL Nat. Genet. 25:338-342 (2000).
RN [34]; RE0053388.
RX PUBMED: 16158053.
RA Lu Z., Luo R. Z., Peng H., Huang M., Nishmoto A., Hunt K. K., Helin K., Liao W. S., Yu Y.
RT E2F-HDAC complexes negatively regulate the tumor suppressor gene ARHI in breast cancer.
RL Oncogene 25:230-239 (2006).
RN [35]; RE0054706.
RX PUBMED: 10428966.
RA Vandel L., Kouzarides T.
RT Residues phosphorylated by TFIIH are required for E2F-1 degradation during S-phase.
RL EMBO J. 18:4280-4291 (1999).
RN [36]; RE0062969.
RX PUBMED: 19318566.
RA Zou J. X., Guo L., Revenko A. S., Tepper C. G., Gemo A. T., Kung H. J., Chen H. W.
RT Androgen-induced coactivator ANCCA mediates specific androgen receptor signaling in prostate cancer.
RL Cancer Res. 69:3339-3346 (2009).
RN [37]; RE0062988.
RX PUBMED: 17998543.
RA Zou J. X., Revenko A. S., Li L. B., Gemo A. T., Chen H. W.
RT ANCCA, an estrogen-regulated AAA+ ATPase coactivator for ERalpha, is required for coregulator occupancy and chromatin modification.
RL Proc. Natl. Acad. Sci. USA 104:18067-18072 (2007).
RN [38]; RE0063166.
RX PUBMED: 18202719.
RA Zalmas L. P., Zhao X., Graham A. L., Fisher R., Reilly C., Coutts A. S., La Thangue N. B.
RT DNA-damage response control of E2F7 and E2F8.
RL EMBO Rep. 9:252-259 (2008).
RN [39]; RE0066230.
RX PUBMED: 20040599.
RA Paik J. C., Wang B., Liu K., Lue J. K., Lin W. C.
RT Regulation of E2F1-induced apoptosis by the nucleolar protein RRP1B.
RL J. Biol. Chem. 285:6348-6363 (2010).
RN [40]; RE0066800.
RX PUBMED: 20220142.
RA Por E., Byun H. J., Lee E. J., Lim J. H., Jung S. Y., Park I., Kim Y. M., Jeoung D. I., Lee H.
RT The cancer/testis antigen CAGE with oncogenic potential stimulates cell proliferation by up-regulating cyclins D1 and E in an AP-1- and E2F-dependent manner.
RL J. Biol. Chem. 285:14475-14485 (2010).
RN [41]; RE0070084.
RX PUBMED: 19917728.
RA Martinez L. A., Goluszko E., Chen H. Z., Leone G., Post S., Lozano G., Chen Z., Chauchereau A.
RT E2F3 is a mediator of DNA damage-induced apoptosis.
RL Mol. Cell. Biol. 30:524-536 (2010).
RN [42]; RE0071006.
RX PUBMED: 20962268.
RA Lin C. Y., Tan B. C., Liu H., Shih C. J., Chien K. Y., Lin C. L., Yung B. Y.
RT Dephosphorylation of nucleophosmin by PP1beta facilitates pRB binding and consequent E2F1-dependent DNA repair.
RL Mol. Biol. Cell 21:4409-4417 (2010).
XX
//