AC T04095
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ID T04095
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DT 11.12.2000 (created); rio.
DT 16.07.2013 (updated); yre.
CO Copyright (C), QIAGEN.
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FA Smad2-L
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SY HMAD-2; MADH2; MADR2; Smad-2; Smad2-Long isoform; Swiss-prot isoform1.
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OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
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GE G002225 SMAD2; HGNC: SMAD2.
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CL C0041; SMAD; 7.1.1.1.2.1.
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SZ 467 AA; 52.3 kDa (cDNA) (calc.).
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SQ MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDE
SQ LEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHR
SQ KGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLV
SQ PRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQS
SQ MDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVD
SQ GFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSP
SQ NCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVK
SQ GWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS
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SC translated from EMBL #U65019
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FT 1 109 
MH1 domain [2].
FT 10 176 
PS51075; MH1.
FT 36 174 
SM00523; dwAneu5.
FT 38 171 
PF03165; MH1 domain.
FT 50 54 
Nuclear Localization Signal, NLS [6].
FT 110 273 
Linker region [2].
FT 268 445 
PF03166; MH2 domain.
FT 272 443 
SM00524; DWB.
FT 274 463 
MH2 domain [2].
FT 274 467 
PS51076; MH2.
FT 464 467 SSXS motif [2].
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SF TGIF T04076 and p300 T01427 compete for interaction with activated Smad2-L [1];
SF interaction with Fast-2 T04100 [1];
SF has a region with 30 aa residues, which is not found in Smad3 or other Smads in mammals and is translated by exon 3 [5];
SF transcripts lacking exon 3 T04442 were found in certain tissues and cells, although the amount is about 1/10 of that containing exon 3 [4] [5];
SF NLS (Nuclear Localization Signal) determines the ligand-induced nuclear translocation [6];
SF MH2 domain contains a central beta sandwich, with a three helix bundle (H3, H4, H5) and a single strand (B1�) on one end, and a loop-helix region (L1, L2, L3, H1) on the other end (PA000015224);
SF specific interaction of five AAs (N381, W368, Y366, I341, F346) with SARA [7];
SF three patterns of transcription and splicing in exon 1exist [4];
SF sequence and activity of promoter region is reported in [4];
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EX blood,basophil granulocyte,Circulatory System & Hematopoietic System,adult; low; Northern blot; mRNA (poly-A); [9].
EX blood,eosinophil granulocyte,Circulatory System & Hematopoietic System,adult; low; Northern blot; mRNA (poly-A); [9].
EX blood,lymphocyte,Circulatory System & Hematopoietic System,adult; low; Northern blot; mRNA (poly-A); [9].
EX blood,monocyte,Circulatory System & Hematopoietic System,adult; low; Northern blot; mRNA (poly-A); [9].
EX blood,neutrophil granulocyte,Circulatory System & Hematopoietic System,adult; low; Northern blot; mRNA (poly-A); [9].
EX brain,,,adult; low; Northern blot; RNA (undefined); [4].
EX brain,,,adult; low; Northern blot; mRNA (poly-A); [9].
EX colon,,,adult; low; Northern blot; mRNA (poly-A); [9].
EX heart,,,adult; high; Northern blot; RNA (undefined); [4].
EX heart,,,adult; high; Northern blot; mRNA (poly-A); [9].
EX kidney (right and left),,,adult; low; Northern blot; mRNA (poly-A); [9].
EX kidney (right and left),,,adult; medium; Northern blot; RNA (undefined); [4].
EX liver,,,adult; low; Northern blot; RNA (undefined); [4].
EX liver,,,adult; low; Northern blot; mRNA (poly-A); [9].
EX lung (right and left),,,adult; low; Northern blot; mRNA (poly-A); [9].
EX lung (right and left),,,adult; medium; Northern blot; RNA (undefined); [4].
EX muscles,,,adult; high; Northern blot; RNA (undefined); [4].
EX muscles,,,adult; high; Northern blot; mRNA (poly-A); [9].
EX ovary (right and left),,,adult; medium; Northern blot; mRNA (poly-A); [9].
EX pancreas,,,adult; medium; Northern blot; RNA (undefined); [4].
EX pancreas,,,adult; medium; Northern blot; mRNA (poly-A); [9].
EX placenta,,,adult; high; Northern blot; RNA (undefined); [4].
EX placenta,,,adult; medium; Northern blot; mRNA (poly-A); [9].
EX prostate gland,,,adult; low; Northern blot; mRNA (poly-A); [9].
EX small intestine,,,adult; medium; Northern blot; mRNA (poly-A); [9].
EX spleen,,,adult; low; Northern blot; mRNA (poly-A); [9].
EX testis (right and left),,,adult; high; Northern blot; mRNA (poly-A); [9].
EX thymus,,,adult; medium; Northern blot; mRNA (poly-A); [9].
XX
FF interaction with TGIF T04076 and HDACs (histone deacetylases) instead of the coactivator p300 T01427 forms a transcriptional repressor complex [1];
FF phosphorylation by TGF-beta signaling pathways [2];
FF associates with FoxH1 (=FAST-1) in mammalian cells in an agonist-dependent manner, and this interaction is mediated via the C domains of the two proteins [3];
FF this association does not require Smad4 T04292 [3];
FF forms a ternary complex with SMAD4 and FoxH1 (=FAST-1) in presence of TGF-beta [3];
FF additional exon, also called TID, in comparison to Smad-3, is responsible for a steric hindrance binding to DNA [10];
FF TGF-beta stimulates nuclear accumulation [9];
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IN T01318 CBP; mouse, Mus musculus.
IN T04100 FoxH1; mouse, Mus musculus.
IN T01427 p300; human, Homo sapiens.
IN T04597 SKIP; human, Homo sapiens.
IN T04096 Smad3-isoform1; human, Homo sapiens.
IN T04292 Smad4; human, Homo sapiens.
IN T04076 TGIF; human, Homo sapiens.
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MX M08897 V$SMAD_Q4.
MX M00792 V$SMAD_Q6.
MX M00974 V$SMAD_Q6_01.
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BS R73967.
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DR TRANSPATH: MO000019779.
DR TRANSCOMPEL: C00200.
DR PATHODB: MT010852.
DR PATHODB: MT010853.
DR PATHODB: MT010854.
DR PATHODB: MT010855.
DR PATHODB: MT010859.
DR PATHODB: MT010862.
DR PATHODB: MT010863.
DR PATHODB: MT010864.
DR EMBL: AF027964; AF027964.
DR EMBL: U65019; HSU65019.
DR UniProtKB: Q15796-1;
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RN [1]; RE0015456.
RX PUBMED: 10199400.
RA Wotton D., Lo R. S., Lee S., Massague J.
RT A Smad transcriptional corepressor
RL Cell 97:29-39 (1999).
RN [2]; RE0015705.
RX PUBMED: 8752209.
RA Eppert K., Scherer S. W., Ozcelik H., Pirone R., Hoodless P., Kim H., Tsui L. C., Bapat B., Gallinger S., Andrulis I. L., Thomsen G. H., Wrana J. L., Attisano L.
RT MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.
RL Cell 86:543-552 (1996).
RN [3]; RE0015707.
RX PUBMED: 9389648.
RA Liu F., Pouponnot C., Massague J.
RT Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes
RL Genes Dev. 11:3157-3167 (1997).
RN [4]; RE0015715.
RX PUBMED: 9503010.
RA Takenoshita S., Mogi A., Nagashima M., Yang K., Yagi K., Hanyu A., Nagamachi Y., Miyazono K., Hagiwara K.
RT Characterization of the MADH2/Smad2 gene, a human Mad homolo responsible for the transforming growth factor-beta and activin signal transduction pathway
RL Genomics 48:1-11 (1998).
RN [5]; RE0016055.
RX PUBMED: 9873005.
RA Yagi K., Goto D., Hamamoto T., Takenoshita S., Kato M., Miyazono K.
RT Alternatively spliced variant of Smad2 lacking exon 3. Comparison with wild-type Smad2 and Smad3.
RL J. Biol. Chem. 274:703-709 (1999).
RN [6]; RE0016203.
RX PUBMED: 10884415.
RA Xiao Z., Liu X., Henis Y. I., Lodish H. F.
RT A distinct nuclear localization signal in the N terminus of Smad 3 determines its ligand-induced nuclear translocation.
RL Proc. Natl. Acad. Sci. USA 97:7853-7858 (2000).
RN [7]; RE0016216.
RX PUBMED: 10615055.
RA Wu G., Chen Y. G., Ozdamar B., Gyuricza C. A., Chong P. A., Wrana J. L., Massague J., Shi Y.
RT Structural basis of Smad2 recognition by the Smad anchor for receptor activation.
RL Science 287:92-97 (2000).
RN [8]; RE0016380.
RX PUBMED: 11074002.
RA Pierreux C. E., Nicolas F. J., Hill C. S.
RT Transforming growth factor beta-independent shuttling of Smad4 between the cytoplasm and nucleus.
RL Mol. Cell. Biol. 20:9041-9054 (2000).
RN [9]; RE0016458.
RX PUBMED: 9006934.
RA Nakao A., Roijer E., Imamura T., Souchelnytskyi S., Stenman G., Heldin C. H., ten Dijke P.
RT Identification of Smad2, a human Mad-related protein in the transforming growth factor beta signaling pathway
RL J. Biol. Chem. 272:2896-2900 (1997).
RN [10]; RE0016515.
RX PUBMED: 10102636.
RA Dennler S., Huet S., Gauthier J. M.
RT A short amino-acid sequence in MH1 domain is responsible for functional differences between Smad2 and Smad3.
RL Oncogene 18:1643-1648 (1999).
RN [11]; RE0018826.
RX PUBMED: 10934479.
RA Xu L., Chen Y.-G., Massague J.
RT The nuclear import function of Smad2 is masked by SARA and unmasked by TGFbeta-dependent phosphorylation
RL Nat. Cell Biol. 2:559-562 (2000).
RN [12]; RE0047907.
RX PUBMED: 12193595.
RA Funaba M., Zimmerman C. M., Mathews L. S.
RT Modulation of Smad2-mediated signaling by extracellular signal-regulated kinase.
RL J. Biol. Chem. 277:41361-41368 (2002).
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