AC T01923
XX
ID T01923
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DT 04.11.1996 (created); ewi.
DT 18.07.2014 (updated); jmh.
CO Copyright (C), QIAGEN.
XX
FA p50
XX
SY 243/NFA-kappaB; H2TF1; KBF1; NF-kappaB (p50); NF-kappaB1; p50.
XX
OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
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CL C0020; Rel.
XX
SF synthesized as a 105 kDa precursor T01924 from which the N-terminal half comprising the Rel domain is released as mature p50 by proteolytic clipping [5];
SF binds to DNA as homo- or heteromer T00588 with, e. g., RelA/p65 or c-Rel [22];
SF the DNA-binding Rel domain comprises two beta-barrel domains the loops of which constitute an extensive DNA contacting surface [17];
SF using a symmetric decameric binding site (GGGAATTCCC), a short alpha-helical segment performs minor groove contacts in a region where augmenting interaction with HMG I(Y) have been observed [17];
XX
CP ubiquitous.
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FF transcriptionally inert DNA-binding subunit of holo-NF-kappaB T00588;
FF trans-activating induced heterodimers with RelA/p65 T00595 [21];
FF hetero-dimerization with Bcl-3 [18];
FF at some elements, however, p50 may exert a constitutive activating function [19];
FF bound to this sequence, p50 adopts a chymotrypsin-resistant conformation [19];
FF constitutive activating complexes in spleen B-cells and thymocytes consist of p50/RelB heterodimers [21];
FF in pre-B cells, mainly p50/p65 heterodimers are induced, whereas in mature B cells, p50/c-Rel heterodimers predominate [22];
FF constitutive NF-kappaB activity in B-cells partly due to enhanced protease activity degrading IkappaB [15];
FF upstream signal transducers for p50 phosphorylation and, presumably, activation may be Raf, Src, H-Ras, Mos [20];
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IN T01928 Bcl-3; human, Homo sapiens.
IN T23418 HDAC1; mouse, Mus musculus.
IN T01940 NF-kappaB1-isoform5; mouse, Mus musculus.
IN T01941 NF-kappaB1-isoform6; mouse, Mus musculus.
IN T01942 NF-kappaB1-isoform7; mouse, Mus musculus.
IN T01923 p50; mouse, Mus musculus.
IN T00595 RelA-p65; mouse, Mus musculus.
IN T01932 RelB; mouse, Mus musculus.
IN T00897 v-Rel; REV-T, reticuloendotheliosis virus (strain T).
XX
MX M00194 V$NFKB_Q6.
MX M00774 V$NFKB_Q6_01.
MX M03557 V$P50_Q6.
XX
BS R02591.
BS R02668.
BS R01766.
BS R24928.
BS R02167.
BS R00699.
BS R20691.
BS R00923.
BS R14486.
BS R27927.
BS R14565.
BS R02189.
BS R01095.
BS R17095.
BS R24927.
BS R30627.
BS R62926.
BS R00877.
BS R14483.
BS R28386.
BS R29918.
BS R01057.
BS R01058.
BS R01060.
BS R02336.
BS R01071.
BS R37830.
BS R37831.
BS R31355.
BS R14616.
BS R38713.
BS R29344.
BS R33510.
BS R33513.
BS R22667.
BS R61241.
BS R14690.
BS R14691.
BS R01448.
BS R14783.
BS R25734.
BS R02217.
BS R01887.
XX
DR TRANSPATH: MO000019358.
XX
RN [1]; RE0000090.
RX PUBMED: 3129195.
RA Baeuerle P. A., Baltimore D.
RT Activation of DNA-Binding Activity in an Apparently Cytoplasmic Precursor of the NF-kB Transcription Factor
RL Cell 53:211-217 (1988).
RN [2]; RE0000169.
RX PUBMED: 2203533.
RA Gilmore T. D.
RT NF-kappaB, KBF1, dorsal, and related matters
RL Cell 62:841-843 (1990).
RN [3]; RE0000174.
RX PUBMED: 1988149.
RA Lewin B.
RT Oncogenic conversion by regulatory changes in transcription factors
RL Cell 64:303-312 (1991).
RN [4]; RE0000176.
RX PUBMED: 2001591.
RA Nolan G. P., Gosh S., Liou H.-C., Tempst P., Baltimore D.
RT DNA binding and IkappaB inhibition of the cloned p65 subunit of NF-kappaB, a rel-related polypeptide
RL Cell 64:961-969 (1991).
RN [5]; RE0000215.
RX PUBMED: 2203532.
RA Ghosh S., Gifford A. M., Riviere L. R., Tempst P., Nolan G. P., Baltimore D.
RT Cloning of the p50 DNA binding subunit of NF-kappaB: homology to rel and dorsal
RL Cell 62:1019-1029 (1990).
RN [6]; RE0000372.
RX PUBMED: 3141147.
RA Wirth T., Baltimore D.
RT Nuclear factor NF-kB can interact functionally with its cognate binding site to provide lymphoid-specific promoter function
RL EMBO J. 7:3109-3113 (1988).
RN [7]; RE0000384.
RX PUBMED: 3322806.
RA Yano O., Kanellopoulos J., Kieran M., Le Bail O., Israel A., Kourilsky P.
RT Purification of KBF1, a common factor binding to both H-2 and beta2-microglobulin enhancers
RL EMBO J. 6:3317-3324 (1987).
RN [8]; RE0000453.
RX PUBMED: 2583102.
RA Lenardo M., Rustgi A. K., Schievella A. R., Bernards R.
RT Suppression of MHC class I gene expression by N-myc through enhancer inactivation
RL EMBO J. 8:3351-3355 (1989).
RN [9]; RE0000658.
RX PUBMED: 2116989.
RA Hagman J., Rudin Ch. M., Haasch D., Chaplin D., Storb U.
RT A novel enhancer in the immunoglobulin lambda locus is duplicated and functionally independent of NFkB
RL Genes Dev. 4:987-992 (1990).
RN [10]; RE0001474.
RX PUBMED: 2196446.
RA Henseling U., Schmidt W., Schoeler H. R., Gruss P., Hatzopoulos A. K.
RT A transcription factor interacting with the class I gene enhancer is inactive in tumorigenic cell lines which suppress major histocompatibility complex class I genes
RL Mol. Cell. Biol. 10:4100-4109 (1990).
RN [11]; RE0001632.
RX PUBMED: 2548081.
RA Shirakawa F., Mizel S. B.
RT In vitro activation and nuclear translocation of NF-kappaB catalyzed by cyclic AMP-dependent protein kinase and protein kinase C
RL Mol. Cell. Biol. 9:2424-2430 (1991).
RN [12]; RE0002064.
RX PUBMED: 2668878.
RA Israel A., Yano O., Logeat F., Kieran M., Kourilsky P.
RT Two purified factors bind to the same sequence in the enhancer of mouse MHC class I genes: one of them is a positive regulator induced upon differentiation of teratocarcinoma cells
RL Nucleic Acids Res. 17:5245 (1989).
RN [13]; RE0002491.
RX PUBMED: 2247438.
RA Rustgi A. K., van't Veer L., Bernards R.
RT Two genes encode factors with NF-kappaB- and H2TF1-like DNA-binding properties
RL Proc. Natl. Acad. Sci. USA 87:8707-8710 (1990).
RN [14]; RE0002609.
RX PUBMED: 3143155.
RA Briskin M., Kuwabara M. D., Sigman D. S., Wall R.
RT Induction of kappa transcription by interferon-gamma without activation of NF-kappaB
RL Science 242:1036-1037 (1988).
RN [15]; RE0004491.
RX PUBMED: 8164680.
RA Miyamoto S., Chiao P. J., Verma I. M.
RT Enhanced IkappaBalpha degradation is responsible for constitutive NF-kappaB activity in mature murine B-cell lines
RL Mol. Cell. Biol. 14:3276-3282 (1994).
RN [16]; RE0004492.
RX PUBMED: 8196632.
RA Zhang Q., Didonato J. A., Karin M., McKeithan T. W.
RT BCL3 encodes a nuclear protein which can alter the subcellular location of NF-kappaB proteins
RL Mol. Cell. Biol. 14:3915-3926 (1994).
RN [17]; RE0004504.
RX PUBMED: 7530332.
RA Ghosh G., van Duyne G., Ghosh S., Sigler P. B.
RT Structure of NF-kappaB p50 homodimer bound to a kappaB site
RL Nature 373:303-310 (1995).
RN [18]; RE0004510.
RX PUBMED: 8330739.
RA Fujita T., Nolan G. P., Liou H.-C., Scott M. L., Baltimore D.
RT The candidate proto-oncogene bcl-3 encodes a transcriptional coactivator that activates through NF-kappaB p50 homodimers
RL Genes Dev. 7:1354-1363 (1993).
RN [19]; RE0004518.
RX PUBMED: 1577272.
RA Fujita T., Nolan G. P., Ghosh S., Baltimore D.
RT Independent modes of transcriptional activation by the p50 and p65 subunits of NF-kappaB
RL Genes Dev. 6:775-787 (1992).
RN [20]; RE0004520.
RX PUBMED: 8255780.
RA Bruder J. T., Heidecker G., Tan T.-H., Derse D., Rapp U. R.
RT Oncogene activation of HIV-LTR-driven expression via the NF-kappaB binding sites
RL Nucleic Acids Res. 21:5229-5234 (1993).
RN [21]; RE0004522.
RX PUBMED: 7692309.
RA Lernbecher T., Mueller U., Wirth T.
RT Distinct NF-kappaB/Rel transcription factors are responsible for tissue-specific and inducible gene activation
RL Nature 365:767-770 (1993).
RN [22]; RE0004531.
RX PUBMED: 8197184.
RA Miyamoto S., Schmitt M. J., Verma I. M.
RT Qualitative changes in the subunit composition of kappa-binding complexes during murine B-cell differentiation
RL Proc. Natl. Acad. Sci. USA 91:5056-5060 (1994).
RN [23]; RE0052059.
RX PUBMED: 11931769.
RA Zhong H., May M. J., Jimi E., Ghosh S.
RT The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1.
RL Mol. Cell 9:625-636 (2002).
XX
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