AC T04122
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ID T04122
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DT 15.12.2000 (created); rio.
DT 05.10.2015 (updated); sla.
CO Copyright (C), QIAGEN.
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FA PREP-1
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SY Pbx regulating protein 1; PBX/Knox1-related homeobox; Prep-1; Prep1.
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OS human, Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; primates
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GE G002132 PKNOX1; HGNC: PKNOX1.
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CL C0006; homeo; 3.1.4.5.1.
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SF conflict in protein sequence between EMBL entry #Y13613 and SWISSPROT entry #P55347: deletion of first pos., pos. 153(152) (K instead of E);
SF TALE (three amino acid loop extension) homeo domain superclass [1];
SF highest homeo domain identity to murine Meis-1T03388 T03389 [2];
SF 64 kDa subunit of the UEF-3 T02839 complex [4];
SF interacts with Pbx-1 T01481 T02087 and binds cooperatively forming a stronger binding complex [4] [5] [6];
SF N-terminal region (HR1 and HR2) shares homology with Meis-1 T03419 and mediates binding to Pbx-1 [4] [5] [6];
SF PREP-1-Pbx-1 complexes are present during mouse embryogenesis [6];
SF forms a ternary complex with Pbx-1a T01481 and HOXB1 T01719 on DNA mediated by Pbx-1a T01481 [6];
SF 52% amino acid identity between human PREP1 T04122 and PREP2 T05155 in total and about 85% amino acid identity in homeo domains [7];
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CP expressed in megakaryocytes cell lines [9].
EX amygdaloid body,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX brain,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX brain,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX brain,,,fetal; high; Northern blot; mRNA (poly-A); [2].
EX caudate nucleus,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX corpus callosum,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX heart,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX hippocampus,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX kidney (right and left),,,adult; medium; Northern blot; mRNA (poly-A); [2].
EX kidney (right and left),,,fetal; high; Northern blot; mRNA (poly-A); [2].
EX liver,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX liver,,,fetal; high; Northern blot; mRNA (poly-A); [2].
EX lung (right and left),,,adult; high; Northern blot; mRNA (poly-A); [2].
EX lung (right and left),,,fetal; high; Northern blot; mRNA (poly-A); [2].
EX muscles,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX pancreas,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX placenta,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX substantia nigra,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX subthalamic nucleus,,,adult; high; Northern blot; mRNA (poly-A); [2].
EX thalamus,,,adult; high; Northern blot; mRNA (poly-A); [2].
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FF may play a role in Down syndrome phenotype [3];
FF enhances HOXB1 T01719 -Pbx-1 T01481 depended transactivation [6];
FF PKNOX2/Pbx-1a heterodimer shows faster DNA-dissociation rate (binding site: R08497) than PREP-1/Pbx-1a heterodimer [7];
FF enhances transcriptional activation of HOXb1/PBX1a heterodimer on HOXB1 gene G002142 autoregulatory element (compare to ) in contrast to its homolog T05155 which reduces it slightly [7];
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IN T01481 Pbx1a; human, Homo sapiens.
IN T02087 Pbx1b; human, Homo sapiens.
IN T00545 POU2F1; human, Homo sapiens.
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MX M07304 V$PBX_Q5.
MX M01459 V$PREP1_01.
MX M07464 V$PREP1_Q3.
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BS R08497.
BS R69707.
BS R72995.
BS R69755.
BS R20301.
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DR TRANSPATH: MO000028025.
DR UniProtKB: P55347;
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RN [1]; RE0014544.
RX PUBMED: 9336443.
RA Burglin T. R.
RT Analysis of TALE superclass homeobox genes (MEIS, PBC, KNOX, Iroquois, TGIF) reveals a novel domain conserved between plants and animals
RL Nucleic Acids Res. 25:4173-4180 (1997).
RN [2]; RE0015485.
RX PUBMED: 9143494.
RA Chen H., Rossier C., Nakamura Y., Lynn A., Chakravarti A., Antonarakis S. E.
RT Cloning of a novel homeobox-containing gene, PKNOX1, and mapping to human chromosome 21q22.3
RL Genomics 41:193-200 (1997).
RN [3]; RE0015486.
RX PUBMED: 9479508.
RA Berthelsen J., Viggiano L., Schulz H., Ferretti E., Consalez G. G., Rocchi M., Blasi F.
RT PKNOX1, a gene encoding PREP1, a new regulator of Pbx activity, maps on human chromosome 21q22.3 and murine chromosome 17B/C
RL Genomics 47:323-324 (1998).
RN [4]; RE0015488.
RX PUBMED: 9482739.
RA Berthelsen J., Zappavigna V., Mavilio F., Blasi F.
RT Prep1, a novel functional partner of Pbx proteins
RL EMBO J. 17:1423-1433 (1998).
RN [5]; RE0015495.
RX PUBMED: 9405651.
RA Knoepfler P. S., Calvo K. R., Chen H., Antonarakis S. E., Kamps M. P.
RT Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1
RL Proc. Natl. Acad. Sci. USA 94:14553-14558 (1997).
RN [6]; RE0015500.
RX PUBMED: 9482740.
RA Berthelsen J., Zappavigna V., Ferretti E., Mavilio F., Blasi F.
RT The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity
RL EMBO J. 17:1434-1445 (1998).
RN [7]; RE0017883.
RX PUBMED: 11972344.
RA Fognani C., Kilstrup-Nielsen C., Berthelsen J., Ferretti E., Zappavigna V., Blasi F.
RT Characterization of PREP2, a paralog of PREP1, which defines a novel sub-family of the MEINOX TALE homeodomain transcription factors
RL Nucleic Acids Res. 30:2043-2051 (2002).
RN [8]; RE0047951.
RX PUBMED: 15138251.
RA Rave-Harel N., Givens M. L., Nelson S. B., Duong H. A., Coss D., Clark M. E., Hall S. B., Kamps M. P., Mellon P. L.
RT TALE homeodomain proteins regulate gonadotropin-releasing hormone gene expression independently and via interactions with Oct-1.
RL J. Biol. Chem. 279:30287-30297 (2004).
RN [9]; RE0048922.
RX PUBMED: 12732210.
RA Okada Y., Matsuura E., Nagai R., Sato T., Watanabe A., Morita I., Doi T.
RT PREP1, MEIS1 homolog protein, regulates PF4 gene expression.
RL Biochem. Biophys. Res. Commun. 305:155-159 (2003).
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