AC T00305
AS T01206.
XX
ID T00305
XX
DT 15.10.1992 (created); ewi.
DT 14.06.2016 (updated); vis.
CO Copyright (C), QIAGEN.
XX
FA GATA-1
XX
SY EF1; EFgammaa; Eryf-1; ERYF1; erythroid transcription factor; GATA binding protein 1; GATA-1; GATA-binding protein 1; GATA1; GF-1; NF-E1; NF-E1 (1); RP23-198C2.6.
XX
OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae; murinae
XX
GE G000512 Gata1.
XX
CL C0004; CC; 2.2.1.1.1.
XX
SF transcription of GATA-1 gene in testis and erythroid cells initiates at different promoters [30] [32];
SF C-terminal but not N-terminal zinc finger required for DNA binding [3];
SF C-terminal but not N-terminal zinc finger required for interaction with Sp1 T00752 [25];
SF N-terminal zinc finger is largely dispensable in nonhemapoietic reporter assays but it is strictly required for terminal erythroid maturation but only partially for megakaryocytic differentiation [34] [18];
SF replacement of the two zinc fingers (202-305) by the single zinc finger of areA T02533 (658-724) creates a protein which is functional in induction of globin genes in cultured embryonal stem cells [33];
SF sequences adjacent to the zinc finger (278-317, 243-304) mediate homo- and heterodimerization (with GATA-2 and -3) [19];
SF large aggregates (foci) have been visualized at certain nuclear bodies [21];
SF gene maps to the proximal region of the X chromosome [27];
SF proerythroblasts contain a transcriptional activator (TAG) of the GATA-1 gene whose activity decreases during terminal differentiation [22];
SF regulates its own expression [15] [4];
SF while almost all sequences outside the DNA binding domain are required for full transcriptional activation the first 66 amino acids (Act1) are most essential [3];
SF translation of GATA-1 mRNA can start from two different AUG codons giving rise to the full length 50 kDa protein (GATA-1) or a shorter 40 kDa product (GATA-1s) lacking the amino acids in front of the methionine at position 84 in GATA-1, GATA-1s can heterodimerize with GATA-1 and it has the same DNA-binding activity as GATA-1, but due to absence of Act1 its ability to activate transcription is reduced [17];
SF in uninduced MEL cells phosphorylated at six N-terminal serine residues (26, 49, 72, 142, 178, 187) after induction with DMSO a 7th serine residue (position 310) gets phosphorylated, but without an effect on its activity [34] [23];
SF the activation domains are mostly dispensable for megakaryocytic or erythroid differentiation [33] [34];
XX
CP erythroid and megakaryocytic cells, [30] [9] [18]; testis, spleen, MA-10 cells [36]; PT18 [46].
EX adrenal gland (right + left),,,adult; none; Northern blot; total RNA; [32].
EX bone marrow,mast cell,,adult; detectable; Northern blot; total RNA; [9].
EX caecum,,,Theiler Stage 22; none; Northern blot; total RNA; [32].
EX caecum,,,Theiler Stage 28; none; Northern blot; total RNA; [32].
EX caecum,,,adult; none; Northern blot; total RNA; [32].
EX cerebellum,,,adult; none; Northern blot; total RNA; [32].
EX cerebrum,,,Theiler Stage 22; none; Northern blot; total RNA; [32].
EX cerebrum,,,Theiler Stage 28; none; Northern blot; total RNA; [32].
EX cerebrum,,,adult; none; Northern blot; total RNA; [32].
EX cerebrum,,,adult; none; Northern blot; total RNA; [32].
EX heart,,,adult; none; Northern blot; total RNA; [32].
EX heart,,,undefined; none; Northern blot; total RNA; [8].
EX kidney (right and left),,,adult; none; Northern blot; total RNA; [8].
EX liver,,,Theiler Stage 20; high; RNA-in situ hybridization (not further specified); RNA (undefined); [18].
EX liver,,,adult; none; Northern blot; total RNA; [32].
EX lung,,,adult; none; Northern blot; total RNA; [32].
EX lung,,,undefined; none; Northern blot; total RNA; [8].
EX mouse, Mus musculus,lymphocyte,,adult; none; Northern blot; total RNA; [32].
EX muscles,myotube,,fetal; none; Northern blot; total RNA; [8].
EX small intestine,,,adult; none; Northern blot; total RNA; [32].
EX spleen,,,adult; detectable; Northern blot; total RNA; [32].
EX stomach,,,undefined; none; Northern blot; total RNA; [8].
EX submandibular gland,,,adult; none; Northern blot; total RNA; [32].
EX testis (right and left),,,Theiler Stage 22; high; Northern blot; total RNA; [32].
EX testis (right and left),,,Theiler Stage 28; low; Northern blot; total RNA; [32].
EX testis (right and left),,,adult; very low; Northern blot; total RNA; [32].
EX testis (right and left),,,adult; very low; Northern blot; total RNA; [32].
EX thymus,,,adult; none; Northern blot; total RNA; [32].
EX uterus,,,undefined; none; Northern blot; total RNA; [8].
XX
FF activator;
FF required for erythroid differentiation [10];
FF GATA-1 negative proerythroblasts undergo developmental arrest and death [31];
FF GATA-2 (mRNA) level is elevated in GATA-1 negative proerythroblasts but does not compensate for GATA-1 deficiency [31];
FF GATA-1 gene expression is stimulated by erythropoietin and required for transcription of other EPO-induced genes [26] [20];
FF synergistic effects of GATA-1 and Sp1 T00752 (or EKLF T01676) (via physical interaction) on gene expression [25];
FF bound and inhibited by estrogen receptor T00261 + beta-estradiol [24] [28];
FF induces the early myeloid cell line 416B to differentiate into megakaryocytes [29];
FF erythroid differentiation of murine embryonic stem cells lacking GATA-1 can be rescued by ectopic expression of chicken GATA-1 T00267, human GATA-3 T00311 or mouse GATA-4 T02532 [33];
FF associated with hematopoietic disorders at the fetal and neonatal stages in individuals with Down syndrome (studies on mice models) [39];
XX
IN T06598 AML-1; mouse, Mus musculus.
IN T14315 AML1; Mammalia.
IN T01318 CBP; mouse, Mus musculus.
IN T10541 E2A; mouse, Mus musculus.
IN T01676 EKLF; mouse, Mus musculus.
IN T00261 ER-alpha; human, Homo sapiens.
IN T01408 Fli-1; mouse, Mus musculus.
IN T10058 Fli-1; mouse, Mus musculus.
IN T02334 FOG; mouse, Mus musculus.
IN T00305 GATA-1; mouse, Mus musculus.
IN T01302 GATA-2; mouse, Mus musculus.
IN T00310 GATA-3; mouse, Mus musculus.
IN T06585 gfi1b; mouse, Mus musculus.
IN T08321 p53-isoform1; human, Homo sapiens.
IN T01806 p53; mouse, Mus musculus.
IN T02235 PEBP2alphaB1; mouse, Mus musculus.
IN T02251 RBTN2; mouse, Mus musculus.
IN T00752 Sp1; mouse, Mus musculus.
IN T08326 Sp1; Mammalia.
IN T18502 Staf; mouse, Mus musculus.
IN T01574 STAT3; mouse, Mus musculus.
IN T16151 Tal-1; mouse, Mus musculus.
XX
MX M00075 V$GATA1_01.
MX M00126 V$GATA1_02.
MX M00127 V$GATA1_03.
MX M00128 V$GATA1_04.
MX M00346 V$GATA1_05.
MX M00347 V$GATA1_06.
MX M07212 V$GATA1_11.
MX M02004 V$GATA1_Q6.
MX M00203 V$GATA_C.
MX M00789 V$GATA_Q6.
XX
BS R23760.
BS R23675.
BS R04764.
BS R04798.
BS R04799.
BS R04903.
BS R23352.
BS R23353.
BS R38239.
BS R38240.
BS R00522.
BS R00523.
BS R00524.
BS R00525.
BS R04289.
BS R04292.
BS R04294.
BS R04296.
BS R23645.
BS R32381.
BS R03487.
BS R28653.
BS R02882.
BS R02883.
BS R02886.
BS R03438.
BS R03442.
BS R03443.
BS R26573.
BS R00569.
BS R30925.
BS R29869.
BS R29870.
BS R29872.
BS R29873.
BS R29874.
BS R29876.
BS R24051.
BS R01247.
BS R01254.
BS R42491.
BS R42492.
BS R32051.
BS R08167.
BS R60186.
BS R42225.
BS R38452.
BS R38456.
BS R27951.
BS R27952.
BS R28638.
BS R08192.
BS R08193.
BS R08194.
BS R22290.
BS R32378.
BS R02890.
BS R24237.
BS R03213.
BS R31332.
BS R08200.
BS R08201.
BS R08203.
BS R08204.
BS R40874.
BS R40878.
BS R33239.
BS R65610.
BS R65611.
BS R03884.
BS R24056.
BS R24057.
BS R73616.
BS R21046.
BS R69961.
BS R39862.
BS R39863.
BS R60130.
BS R21700.
BS R21701.
BS R20755.
BS R20756.
XX
DR TRANSPATH: MO000005556.
DR TRANSCOMPEL: C00477.
DR TRANSCOMPEL: C00612.
DR UniProtKB: P17679;
XX
RN [1]; RE0000167.
RX PUBMED: 2225071.
RA Orkin S. H.
RT Globin gene regulation and switching: Circa 1990
RL Cell 63:665-672 (1990).
RN [2]; RE0000603.
RX PUBMED: 2461328.
RA Wall L., deBoer E., Grosveld F.
RT The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein
RL Genes Dev. 2:1089-1100 (1989).
RN [3]; RE0000728.
RX PUBMED: 2276623.
RA Martin D. I. K., Orkin St. H.
RT Transcriptional activation and DNA binding by the erythroid factor GF-1/NF-E1/Eryf 1
RL Genes Dev. 4:1886-1898 (1990).
RN [4]; RE0000752.
RX PUBMED: 2044960.
RA Tsai S.-F., Strauss E., Orkin S. H.
RT Functional analysis and in vivo footprinting implicate the erythroid transcription factor GATA-1 as a positive regulator of its own promoter
RL Genes Dev. 5:919-931 (1991).
RN [5]; RE0001000.
RX PUBMED: 2037597.
RA Porcher C., Pitiot G., Plumb M., Lowe S., de Verneuil H., Grandchamp B.
RT Characterization of hypersensitive sites, protein-binding motifs, and regulatory elements in both promoters of the mouse porphobilinogen deaminase gene
RL J. Biol. Chem. 266:10562-10569 (1991).
RN [6]; RE0001256.
RX PUBMED: 2761541.
RA Barnhart K. M., Kim C. G., Sheffery M.
RT Purification and characterization of an erythroid cell-specific factor that binds the murine alpha- and beta-globin genes
RL Mol. Cell. Biol. 9:2606-2614 (1989).
RN [7]; RE0001472.
RX PUBMED: 1990287.
RA Evans T., Felsenfeld G.
RT trans-activation of a globin promoter in nonerythroid cells
RL Mol. Cell. Biol. 11:843-853 (1991).
RN [8]; RE0001788.
RX PUBMED: 2725678.
RA Tsai S.-F., Martin D. I. K., Zon L. I., D'Andrea A. D., Wong G. G., Orkin S. H.
RT Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells
RL Nature 339:446-451 (1989).
RN [9]; RE0001849.
RX PUBMED: 2320112.
RA Martin D. I. K., Zon L. I., Mutter G., Orkin S. H.
RT Expression of an erythroid transcription factor in megakaryocytic and mast cell lineages
RL Nature 344:444-447 (1990).
RN [10]; RE0001930.
RX PUBMED: 1987478.
RA Pevny L., Simon M. C., Robertson E., Klein W. H., Tsai S.-F., D'Agati V., Orkin S. H., Constantini F.
RT Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1
RL Nature 349:257-260 (1991).
RN [11]; RE0002020.
RX PUBMED: 2911469.
RA Mignotte V., Wall L., deBoer E., Grosveld F., Romeo P.-H.
RT Two tissue-specific factors bind to the erythroid promoter of the human porphobilinogen deaminase gene
RL Nucleic Acids Res. 17:37-54 (1989).
RN [12]; RE0002078.
RX PUBMED: 1698280.
RA Fucharoen S., Shimizu K., Fukumaki Y.
RT A novel C-T transition within the distal CCAAT motif of the Ggamma-globin gene in the Japanese HPFH: implication of factor binding in elevated fetal globin expression
RL Nucleic Acids Res. 18:5245 (1990).
RN [13]; RE0002473.
RX PUBMED: 2014222.
RA Hannon R., Evans T., Felsenfeld G., Gould H.
RT Structure and promoter activity of the gene for the erythroid transcription factor GATA-1
RL Proc. Natl. Acad. Sci. USA 88:3004-3008 (1991).
RN [14]; RE0002765.
RX PUBMED: 2355926.
RA Frampton J., Walker M., Plumb M., Harrison P. R.
RT Synergy between the NF-E1 erythroid-specific transcription factor and the CACCC factor in the erythroid-specific promoter of the human porphobilinogen deaminase gene
RL Mol. Cell. Biol. 10:3838-3842 (1990).
RN [15]; RE0002885.
RX PUBMED: 1656391.
RA Nicolis S., Bertini C., Ronchi A., Crotta S., Lanfranco L., Moroni E., Giglioni B., Ottolenghi S.
RT An erythroid specific enhancer upstream to the gene encoding the cell-type specific transcription factor GATA-1
RL Nucleic Acids Res. 19:5285-5291 (1991).
RN [16]; RE0002959.
RX PUBMED: 8321207.
RA Merika M., Orkin S. H.
RT DNA-binding specificity of GATA family transcription factors
RL Mol. Cell. Biol. 13:3999-4010 (1993).
RN [17]; RE0004779.
RX PUBMED: 8524811.
RA Calligaris R., Bottardi S., Cogoi S., Apezteguia I., Santoro C.
RT Alternative translation initiation site usage results in two functionally distincts forms of the GATA-1 tanscription factor
RL Proc. Natl. Acad. Sci. USA 92:11598-11602 (1995).
RN [18]; RE0006528.
RX PUBMED: 9230307.
RA Tsang A. P., Visvader J. E., Turner C. A., Fujiwara Y., Yu C., Weiss M. J., Crossley M., Orkin S. H.
RT FOG, a multitype zinc finger protein, acts as a cofactor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation
RL Cell 90:109-119 (1997).
RN [19]; RE0006589.
RX PUBMED: 7739529.
RA Crossley M., Merika M., Orkin S. H.
RT Self-association of the erythroid transcription factor GATA-1 mediated by its zinc finger domains
RL Mol. Cell. Biol. 15:2448-2456 (1995).
RN [20]; RE0007084.
RX PUBMED: 7607218.
RA Busfield S. J., Spadaccini A., Riches K. J., Tilbrook P. A., Klinken S. P.
RT The major erythroid DNA-binding protein GATA-1 is stimulated by erythropoietin but not by chemical inducers of erythroid differentiation
RL Eur. J. Biochem. 230:475-480 (1995).
RN [21]; RE0007093.
RX PUBMED: 8617207.
RA Elefanty A. G., Antoniou M., Custodio N., Carmo-Fonseca M., Grosveld F. G.
RT GATA transcription factors associate with a novel class of nuclear bodies in erythroblasts and megakaryocytes
RL EMBO J. 15:319-333 (1996).
RN [22]; RE0008992.
RX PUBMED: 8164666.
RA Baron M. H., Farrington S. M.
RT Positive regulators of the lineage-specific transcription factor GATA-1 in differentiating erythroid cells
RL Mol. Cell. Biol. 14:3108-3114 (1994).
RN [23]; RE0009302.
RX PUBMED: 8206977.
RA Crossley M., Orkin S. H.
RT Phosphorylation of the erythroid transcription factor GATA-1
RL J. Biol. Chem. 269:16589-16596 (1994).
RN [24]; RE0010521.
RX PUBMED: 7760810.
RA Blobel G. A., Sieff C. A., Orkin S. H.
RT Ligand-dependent repression of the erythroid transcription factor GATA-1 by the estrogen receptor
RL Mol. Cell. Biol. 15:3147-3153 (1995).
RN [25]; RE0010577.
RX PUBMED: 7739528.
RA Merika M., Orkin S. H.
RT Functional synergy and physical interactions of the erythroid transcription factor GATA-1 with the Krueppel family proteins Sp1 and EKLF
RL Mol. Cell. Biol. 15:2437-2447 (1995).
RN [26]; RE0011673.
RX PUBMED: 7896801.
RA Taxman D. J., Wojchowski D. M.
RT Erythropoietin-induced transcription at the murine betamaj-globin promoter
RL J. Biol. Chem. 270:6619-6627 (1995).
RN [27]; RE0013395.
RX PUBMED: 2004781.
RA Chapman V. M., Stephenson D. A., Mullins L. J., Keitz B. T., Disteche C., Orkin S. H.
RT Linkage of the erythroid transcription factor gene (Gf-1) to the proximal region of the X chromosome of mice.
RL Genomics 9:309-313 (1991).
RN [28]; RE0013427.
RX PUBMED: 8657144.
RA Blobel G. A., Orkin S. H.
RT Estrogen-induced apoptosis by inhibition of the erythroid transcription factor GATA-1.
RL Mol. Cell. Biol. 16:1687-1694 (1996).
RN [29]; RE0013429.
RX PUBMED: 1385117.
RA Visvader J. E., Elefanty A. G., Strasser A., Adams J. M.
RT GATA-1 but not SCL induces megakaryocytic differentiation in an early myeloid line.
RL EMBO J. 11:4557-4564 (1992).
RN [30]; RE0013430.
RX PUBMED: 7925299.
RA Cairns L. A., Crotta S., Minuzzo M., Moroni E., Granucci F., Nicolis S., Schiro R., Pozzi L., Giglioni B., Ricciardi-Castagnoli P., Ottolenghi S.
RT Immortalization of multipotent growth-factor dependent hemopoietic progenitors from mice transgenic for GATA-1 driven SV40 tsA58 gene.
RL EMBO J. 13:4577-4586 (1994).
RN [31]; RE0013432.
RX PUBMED: 7926723.
RA Weiss M. J., Keller G., Orkin S. H.
RT Novel insights into erythroid development revealed through in vitro differentiation of GATA-1- embryonic stem cells.
RL Genes Dev. 8:1184-1197 (1994).
RN [32]; RE0013438.
RX PUBMED: 8464479.
RA Ito E., Toki T., Ishihara H., Ohtani H., Gu L., Yokoyama M., Engel J. D., Yamamoto M.
RT Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis.
RL Nature 362:466-468 (1993).
RN [33]; RE0013446.
RX PUBMED: 7823931.
RA Blobel G. A., Simon M. C., Orkin S. H.
RT Rescue of GATA-1-deficient embryonic stem cells by heterologous GATA-binding proteins.
RL Mol. Cell. Biol. 15:626-633 (1995).
RN [34]; RE0013447.
RX PUBMED: 7823932.
RA Visvader J. E., Crossley M., Hill J., Orkin S. H., Adams J. M.
RT The C-terminal zinc finger of GATA-1 or GATA-2 is sufficient to induce megakaryocytic differentiation of an early myeloid cell line.
RL Mol. Cell. Biol. 15:634-641 (1995).
RN [35]; RE0025804.
RX PUBMED: 14668799.
RA Watamoto K., Towatari M., Ozawa Y., Miyata Y., Okamoto M., Abe A., Naoe T., Saito H.
RT Altered interaction of HDAC5 with GATA-1 during MEL cell differentiation.
RL Oncogene 22:9176-84 (2003).
RN [36]; RE0027116.
RX PUBMED: 9514155.
RA Feng Z. M., Wu A. Z., Chen C. L.
RT Testicular GATA-1 factor up-regulates the promoter activity of rat inhibin alpha-subunit gene in MA-10 Leydig tumor cells.
RL Mol. Endocrinol. 12:378-90 (1998).
RN [37]; RE0031242.
RX PUBMED: 11544260.
RA Ingley E., Chappell D., Poon S. Y., Sarna M. K., Beaumont J. G., Williams J. H., Stillitano J. P., Tsai S., Leedman P. J., Tilbrook P. A., Klinken S. P.
RT Thyroid hormone receptor-interacting protein 1 modulates cytokine and nuclear hormone signaling in erythroid cells.
RL J. Biol. Chem. 276:43428-34 (2001).
RN [38]; RE0034905.
RX PUBMED: 15173587.
RA Collavin L., Gostissa M., Avolio F., Secco P., Ronchi A., Santoro C., Del Sal G.
RT Modification of the erythroid transcription factor GATA-1 by SUMO-1.
RL Proc. Natl. Acad. Sci. USA 101:8870-5 (2004).
RN [39]; RE0035245.
RX PUBMED: 15895080.
RA Li Z., Godinho F. J., Klusmann J. H., Garriga-Canut M., Yu C., Orkin S. H.
RT Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1.
RL Nat. Genet. 37:613-619 (2005).
RN [40]; RE0045775.
RX PUBMED: 12556498.
RA Starck J., Cohet N., Gonnet C., Sarrazin S., Doubeikovskaia Z., Doubeikovski A., Verger A., Duterque-Coquillaud M., Morle F.
RT Functional cross-antagonism between transcription factors FLI-1 and EKLF
RL Mol. Cell. Biol. 23:1390-402 (2003).
RN [41]; RE0046278.
RX PUBMED: 12359731.
RA Holmes M. L., Bartle N., Eisbacher M., Chong B. H.
RT Cloning and analysis of the thrombopoietin-induced megakaryocyte-specific glycoprotein VI promoter and its regulation by GATA-1, Fli-1, and Sp1
RL J. Biol. Chem. 277:48333-41 (2002).
RN [42]; RE0046907.
RX PUBMED: 9334239.
RA Surinya K. H., Cox T. C., May B. K.
RT Transcriptional regulation of the human erythroid 5-aminolevulinate synthase gene. Identification of promoter elements and role of regulatory proteins
RL J. Biol. Chem. 272:26585-94 (1997).
RN [43]; RE0047114.
RX PUBMED: 9473675.
RA Kambe M., Miyamoto Y., Hayashi M.
RT Characterization of the integrin alpha(v) gene promoter in mice
RL Biochim. Biophys. Acta 1395:209-19 (1998).
RN [44]; RE0047322.
RX PUBMED: 15673499.
RA Ezoe S., Matsumura I., Gale K., Satoh Y., Ishikawa J., Mizuki M., Takahashi S., Minegishi N., Nakajima K., Yamamoto M., Enver T., Kanakura Y.
RT GATA transcription factors inhibit cytokine-dependent growth and survival of a hematopoietic cell line through the inhibition of STAT3 activity.
RL J. Biol. Chem. 280:13163-13170 (2005).
RN [45]; RE0049146.
RX PUBMED: 16287841.
RA Schuh A. H., Tipping A. J., Clark A. J., Hamlett I., Guyot B., Iborra F. J., Rodriguez P., Strouboulis J., Enver T., Vyas P., Porcher C.
RT ETO-2 associates with SCL in erythroid cells and megakaryocytes and provides repressor functions in erythropoiesis.
RL Mol. Cell. Biol. 25:10235-10250 (2005).
RN [46]; RE0052669.
RX PUBMED: 12496417.
RA Maeda K., Nishiyama C., Tokura T., Akizawa Y., Nishiyama M., Ogawa H., Okumura K., Ra C.
RT Regulation of cell type-specific mouse Fc epsilon RI beta-chain gene expression by GATA-1 via four GATA motifs in the promoter.
RL J. Immunol. 170:334-340 (2003).
XX
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